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Plant Tissue Culture What Is plant tissue culture? Definition Basis for Plant Tissue Culture Two Hormones Affect Plant Differentiation: Auxin: Stimulates Root ...
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Our Learning Objectives in relation to Tissue Culture. Reasons for propagating plants by tissue culture are stated. The culture process is described
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Cell And Tissue Culture Mammalian Cells Advanced Higher Biology Why is it useful? Gene manipulation Culturing mammalian cells for cancer studies Producing new plants ...
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Tissue Culture Unit one Cell and Molecular Biology Advanced Higher Biology Arrangements Differentiation of cells into tissues and organs. Cells undergo ...
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Plant Tissue Culture in vitro propagation Rapid (compared to outdoors) Asexual Used for 1. Micropropagation 2. Regeneration Vegetative (asexual) Propagation Occurs in ...
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Major development’s in cell culture technology Why is cell culture used for? Contd…. Tissue culture Primary culture Continous cell lines Types of cells Culture ...
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Plant Tissue Culture T.C. Refers to technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium ...
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Brief History of Tissue Culture. 1885. Wilhelm Roux. Embryonic chick cells maintained alive in a . saline solution. 1907. Ross Harrison. Frog Embryo nerve fibre
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Tissue culture provided another level of genetic variation: somaclonal variation. Ability to cut and ligate DNA: gene mapping and cloning techniques.
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Plant Tissue Culture What is it? Tissue culture is the term used for “the process of growing cells artificially in the laboratory” Tissue culture produces clones ...
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Plant Tissue Culture Definition: The term plant tissue culture is described in vitro and aseptic cultivation of any plant part on nutrient medium.
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Tissue Culture Methodology Historical Perspective Early 20th Century Technique Antibiotics and Laminar Flow Hoods Allow for Tissue Culturing to Grow Exponentially ...
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In vitro culture, Cell Culture, Tissue Culture, Organ Culture, Embryo Culture? In Vitro Culture The culture of organized living material cell under artificial ...
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Genetic variations in plants that have been produced by plant tissue culture and can be detected as genetic or phenotypic traits. Basic Features of Somaclonal ...
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Title: NASA/NIH CENTER FOR THREE-DIMENSIONAL TISSUE CULTURE Author: Joshua Zimmerberg Last modified by: druganj Created Date: 11/3/2003 5:38:44 AM
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Plant Tissue Cultures Conditions required for plant tissue cultures An original stock of cells. The use of aseptic techniques when preparing media and cultures.
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Plant Tissue Culture 102-1 Prof. H. C Wu History What’s the history? 1838 – Schwann and Schleiden put forward the theory which states that cells are totipotent ...
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Plant Tissue Culture-generation of whole plants from a single cell or tissue-Biotech II * * * Picture in back of your protocol packet shows detail re: how the ...
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Grow mammalian vs bacterial cells. For both need: For bacteria: Tryptone, yeast extract, salt +/- antibiotic, agar. For MES cells: DMEM, calf serum, glutamine, penn/strep
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Title: Lecture 2: Applications of Tissue Culture to Plant Improvement Author: Steve R. King Last modified by: ibm Created Date: 3/13/2003 5:16:30 AM
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Plant Tissue Culture Media What’s really important? Plant Tissue Culture Why does it work? Plant cells – Dedifferentiate Plant cell division- Somatic cells are ...
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Title: Lecture 2: Applications of Tissue Culture to Plant Improvement Author: Steve R. King Last modified by: Taryono Dr Created Date: 3/13/2003 5:16:30 AM
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Growing Cells in Culture Part 1: Terminology Some volumes that do not need to be exact—but follow our recommendations until you are comfortable Rinsing volume of ...
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Culturing (micropropagating) Plant Tissue - the steps. Stage 0 – Selection & preparation of the mother plant. sterilization of the plant tissue takes place
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GLASSWARE WASHING AND STORAGE AREA. Large sink (two sinks are preferable) Plant Tissue Culture
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What is Plant Tissue Culture? Anther and microspore culture Production of haploid plants Production of homozygous diploid lines through chromosome doubling, ...
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Title: Tissue Culture Techniques Lab Author: MAHMOUD Last modified by: Mahmoud Created Date: 9/6/2012 7:46:51 PM Document presentation format: عرض على ...
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Cell And Tissue Culture * * * * * * Why is it useful? Gene manipulation Culturing mammalian cells for cancer studies Producing new plants through tissue culture What ...
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Basic parts of bioreactor Bioreactors for cultivation of plant cells Slide 20 Slide 21 Slide 22 Plant Tissue Culture- Different Approaches for Production of ...
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Biology Of Cultured Cells Does Culturing Reflect Reality Culturing Deviates From In Vivo Environment 3-D matrix is disrupted (collagen, cell-cell contact ...
Date added: November 11, 2011 - Views: 33
Lesson 6 Propagating Plants by Tissue Culture Next Generation Science/Common Core Standards Addressed! HS‐LS1‐1. Construct an explanation based on evidence for ...
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Environment It is fairly obvious that the environment in which tissue culture is ... that has been used in conjunction with the culture. (5) If the contamination ...
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Tissue Culture Applications Micropropagation Germplasm preservation Somaclonal variation & mutation selection Embryo Culture Haploid & Dihaploid Production
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Lecture 8 Plant Tissue Culture & Applications What is it? Tissue culture is the term used for “the process of growing cells artificially in the laboratory” (OSMS ...
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Agrobacterium-mediated transfer Plant tissue culture is the cultivation of plant cells or tissues on specifically formulated nutrient enriched media.
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Introduction to Tissue culture Sompol Tapechum M.D., Ph.D. Department of Physiology Faculty of Medicine Siriraj Hospital Experimental Methods 2006 * Temperature The ...
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Basic Principles & Protocol in Plant Tissue Culture Chapter 9 Objective Be familiar with the protocol in plant tissue culture Get know the application of aseptic ...
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Agricultural Biotechnology in Sri Lanka Kumudu Fernando Seed Certification and plant protection Centre, Department of Agriculture Tissue Culture Micropropagation -1 ...
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title: effects of different gelling agents in tissue culture of citrus name: lucas omondi adm. number: a22/0076/2009 supervised by: dr. t. magomere
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Plant Tissue Culture collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions mostly used to produce clones Plant ...
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An Ecological Perspective (BIOL 346) Talk Seven: GMOs and the Environment And then?..... What is the last step?..... Tissue culture The basics!
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Modern Methods for Influenza Detection and ... months Laboratory issues Laboratory safety Tissue culture techniques Rapid test kits HA/HI sub-typing Immuno ...
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Plant propagation... Sexual methods - seed propagation Asexual methods rooting cuttings layering; air layering grafting specialized structures tissue culture ...
Date added: October 12, 2011 - Views: 204
Monoclonal Antibody Production Cathy Langford, ... The vitro tissue culture is the method used when the cells are places in culture outside the mouse's body in a flask.
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principles of crop production abt-320 (3 credit hours)) lecture 3 lecture-wise course breakup tissue culture major components of tissue culture media
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Factors to Consider in Plant Tissue Culture Propagation The species and variety of plant material The medium and medium ingredients The preparation of plant samples, ...
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Title: Nutritional Requirements of Tissue culture Author: Internet Center Last modified by: Internet Center Created Date: 1/6/2007 9:00:36 AM Document presentation format
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MICROPROPAGATION This is the bulk production of clonal plants for rapid propagation. It is an important application of tissue culture technology in plant breeding.
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Virology Lab 6 Definitive ... and breaking of chromosomes Rounding up and detachment of cultured cells Inclusion bodies Quantification of CPE Tissue Culture Infective ...
Date added: October 15, 2011 - Views: 66