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SOUTHERN BLOTTING - Gadjah Mada University

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SOUTHERN BLOTTING - Gadjah Mada University

SOUTHERN BLOTTING The technique was developed by E.M. Southern in 1975. ... Would you watch the animation? A. Yes B. No SUMMARY OF PROCEDURE 1. Extract and purify DNA from cells 2. DNA is restricted with enzymes 3. Sort by electrophoresis 4. Denature DNA 5. Transfer to nitrocellulose paper 6.

http://faperta.ugm.ac.id/download/pak_tar/genetika-molekuler/presentasi/SOUTHERN%20BLOTTING.ppt

Date added: June 25, 2012 - Views: 165

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Genetics Techniques: RFLP & PCR - fuhsd

Southern Blotting A method to visualize specific segments of DNA– usually a ... with radioactive probe for specific segment Wash away unbound probe Detect probes using x-ray film autoradiograph RFLP Animations Animation #1 Animation #2 Polymerase Chain Reaction PCR allows scientists ...

http://web.fuhsd.org/pamela_chow/apbio/lectures/DNA%20Replication%20&%20Genetics/Genetics%20Techniques.ppt

Date added: April 15, 2012 - Views: 310

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Introduction to Northern/Southern Blotting

Introduction to Southern Hybridization Michael Melzer Plant & Environmental Protection Sciences University of Hawaii at Manoa Outline History/Background Info Goals of Southern hybridization Example Other applications History/Background ‘Southern’ hybridization named after Sir Edwin Southern ...

http://abe.leeward.hawaii.edu/WebSharing/Southern_Melzer.ppt

Date added: November 6, 2011 - Views: 218

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Southern Blotting - Lakeside School

Southern Blotting INVENTED BY BRITISH BIOCHEMIST EDWIN SOUTHERN * Purpose Used to determine whether a particular nucleotide sequence is present in different sources of DNA, and also to discover the size of the restriction fragment that contains the specific sequence Example Sickle Cell Can ...

http://teachers.lakesideschool.org/us/science/parry/documents/SouthernNorthernandWesternBlotting.ppt

Date added: November 28, 2011 - Views: 238

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VNTR, STR and RFLP - Austin Community College District

Size determined by gel electrophoresis and Southern blotting to produce pattern of ... Activities Review tutorial on Western Blot http://tinyurl.com/awaut3 Review VNTR animation http://tinyurl.com/dawjau Review DNA fingerprinting http://tinyurl.com/co2yml Review DNA forensics Problem set 1 ...

http://www.austincc.edu/mlt/mdtech/BlottingMethods.ppt

Date added: September 13, 2011 - Views: 167

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Slide 1

Restriction Enzyme Digestion & Southern Blotting of DNA

http://site.iugaza.edu.ps/mlaqqan/files/2013/11/Restriction-enzymes-Southern-blotting-mod.ppt

Date added: December 5, 2013 - Views: 2

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… Get Document - Gavilan College -> Faculty / Staff Web Pages

... DNA cloning yields multiple copies of a gene or other DNA ... allele Probe base-pairs with fragments Nitrocellulose blot 4 5 Radioactively labeled probe for -globin gene Southern blotting animation Northern blotting animation Fig. 20-13 TECHNIQUE RESULTS Gel electrophoresis ...

http://hhh.gavilan.edu/rmorales/documents/20_Lecture_Presentationshortversion.ppt

Date added: September 4, 2011 - Views: 109

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Genetic engineering and the Human Genome Project

After gel electrophoresis Filter paper blots DNA off Radioactive probes added Autoradiography pinpoints sequence Southern Blot animation The scientific ... DNA fingerprinting Let’s take a brief look at some of these… What is gel electrophoresis? What is Southern blotting? The ...

http://skywalker.cochise.edu/ashraft/web/BIO205%20ppt/Lectures%202010/9%20biotechnology.ppt

Date added: May 22, 2013 - Views: 23

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PowerPoint Presentation

Scene: Animation of labeled probe fragment pairing with target ... Animation of DNA/RNA probe preparations Animation of Complementary Base-Pairing Live shot of Researcher--Segue to Blotting Southern Blot Transfer Preparation Animation of Transfer Process Crosslink of DNA to Nitrocellulose ...

http://multimedia.mcb.harvard.edu/powerpoint/No-So.ppt

Date added: November 7, 2011 - Views: 31

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الشريحة 1 - الصفحات الشخصية

Steps in Southern Blotting Southern Blotting Animation Advantages and Disadvantages of Southern Blotting Advantages The exact location of a disorder does not need to be known Disadvantages They are rather time consuming Needs high quality/quantity DNA Radioactive Some tasks previously ...

http://site.iugaza.edu.ps/yabdelal/files/Exp._7_-_Restriction_enzymes__Southern_blotting___mod.ppt

Date added: August 13, 2014 - Views: 1

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Lecture #9 Date _____

DNA Technology Now it gets real….

http://www2.moundsviewschools.org/irondale/userfiles/helmj/16.dna_technology_and_genomics.ppt

Date added: August 24, 2014 - Views: 1

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Microbiology - Des Moines Area Community College, Iowa ...

Scientific Applications Understanding DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern Blotting Southern Blotting Forensic Microbiology PCR Primer for a specific ... Recombinant DNA Technology Table 9.1 Figure 9.2 Figure 9.3 ANIMATION PCR: ...

http://www.library.dmacc.cc.ia.us/instructors/ashelgeson/A_PowerPoint/a_Lecture_Outline/_START-HERE_ch09_lecture.ppt

Date added: October 26, 2011 - Views: 86

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Chapter 20

... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ... Base pairing occurs. 2 Animation: ...

http://www.course-notes.org/sites/www.course-notes.org/files/past/20_Lecture_Presentation_0.ppt

Date added: May 13, 2013 - Views: 19

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Chapter 20

Chapter 20 Biotechnology

http://www.nscsd.org/webpages/gbroton/files/ch%2020%20biotechnology.ppt

Date added: April 3, 2014 - Views: 1

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PowerPoint Presentation

This would take about 3 hours to perform in lab. PCR Animation DNA Fingerprinting Used to identify individuals by their repeat regions (usually STR) regions: ... (Southern blotting) A radioactive primer is designed that will be complementary to unique regions (STR, etc, regions).

http://webpages.sou.edu/~chapman/CH300S10/Forensic%20DNA%20Analysis%20(student).ppt

Date added: February 13, 2013 - Views: 37

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PowerPoint Presentation

Explain how serological tests and phage typing can be used to identify ... Examples of Applications: Southern blotting, DNA chips, and FISH Nucleic Acid Hybridization ... Fig 10.18a–b Add DNA or RNA probe attached to fluorescent dye for S. aureus Dichotomous Key ANIMATION ...

http://lpc1.clpccd.cc.ca.us/lpc/zingg/Micro/lecture%20notes/M_T_Ch10_Classification_SS10.ppt

Date added: December 5, 2011 - Views: 79

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Slide 1

Polymerase Chain Reaction Animation PCR http://www.dnalc.org/ddnalc/resources/pcr.html Gel electrophoresis a method that ... http://207.207.4.198/pub/flash/4/4.html `http://www.dnalc.org/ddnalc/resources/electrophoresis.html Southern blotting Southern blotting was named after Edward M ...

http://utechdmd2015.wikispaces.com/file/view/unit4_Molecular_Biology_techniques_revised_1_.ppt

Date added: March 22, 2012 - Views: 35

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PowerPoint Presentation

* * Figure 20.11 Research Method: Southern Blotting of DNA Fragments * * Figure 20.12 Research Method: ... The fragments are mixed, and DNA ligase is added to bond the fragment sticky ends © 2011 Pearson Education, Inc. Animation: ...

http://faculty.gfcmsu.edu/rpeffer/BIOB%20160/Lecture%20Notes/20_Lecture_Presentation.ppt

Date added: March 16, 2013 - Views: 21

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AP Bio Chapter 20 pt. 2 - Cardinal Newman

For the Discovery Video Transgenics, go to Animation and Video Files. ... is used to analyze nucleic acids and to distinguish between two alleles of a gene Describe and distinguish between the Southern blotting procedure, Northern blotting procedure, ...

http://www.cardinalnewman.org/s/206/images/editor_documents/ap_biology_chapter_20_pt._2.ppt

Date added: June 28, 2013 - Views: 4

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DNA Technology Lecture - Green River Community College

Figure 20.10 Restriction fragment analysis by Southern blotting Steps 2 & 3. How to Insert the Gene of Interest into the R-Plasmid See next 3 figures and animation Lyse bacteria with detergent to release the R-plasmid (e.g ...

http://instruction.greenriver.edu/kmarr/Biology%20211/Lecture%20Notes/Week%2011/1_Ch%2020%20DNA%20Technology_Campbell%206e_F06.ppt

Date added: October 3, 2014 - Views: 1

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Introduction to Biotechnology - Kennesaw State University ...

Hybridization DNA hybridization Southern blotting (pg75) Southern Blot ... Primer extension Fig. 3.8 The Polymerase Chain Reaction Go to Animation courtesy of the following websites: References http://faculty.plattsburgh.edu/donald.slish/PCRmov.html ...

http://science.kennesaw.edu/~pachar/Btech3100/Chapter%203%20CONTINUE%20Recombinant%20DNA%20Technology.ppt

Date added: July 1, 2012 - Views: 22

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PowerPoint Presentation

Describe how microorganisms can be identified by nucleic acid hybridization, Southern blotting ... taxon, and phylogeny. List the characteristics of the Bacteria, Archaea, and Eukarya domains. Differentiate among eukaryotic, prokaryotic, and ... and FISH Figs. 10.17 and 10.18 PLAY Animation ...

http://lpc1.clpccd.cc.ca.us/lpc/zingg/Micro/lecture%20notes/M_T_Ch10_Classification_s.ppt

Date added: June 12, 2012 - Views: 68

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Chapter 20 - SCT JJ's Sciences

Several techniques are used to analyze the DNA of genes: Gel Electrophoresis, restriction fragment analysis, Southern blotting, DNA sequencing. ... Figure 20.18 Reproductive cloning of a mammal by nuclear transplantation For the Discovery Video Cloning, go to Animation and Video Files.

http://jj-sct-bio.weebly.com/uploads/2/4/6/5/2465445/20_lecture_presentation.ppt

Date added: February 9, 2012 - Views: 44

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Chapter 20

Chapter 20 Biotechnology * Figure 20.9 Gel electrophoresis * Figure 20.9 Gel electrophoresis * Figure 20.9 Gel electrophoresis * * Figure 20.10 Using restriction fragment analysis to distinguish the normal and sickle-cell alleles of the β-globin gene * * Figure 20.11 Southern blotting of DNA ...

http://www.etsu.edu/coe/uschool/faculty/tadlockd/apbiology/chp_20_pwrpt.ppt

Date added: May 3, 2014 - Views: 12

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PowerPoint Presentation

Web animation of a cDNA microarray experiment Yeast genome on a chip Brief outline of steps for ... (e.g. by X-ray for radioactive probes) Sample Autoradiogragh (Gel) Types of Blots Southern ... 3’ 3’ – CTTAAG – 5’ (cut) The Southern blotting technique * Title ...

http://statwww.epfl.ch/davison/teaching/Microarrays/lec/week02.ppt

Date added: October 15, 2011 - Views: 98

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Biotechnology and Genetic Engineering-PBIO 450/550

PBIO 427/527: Molecular Genetics Lecture 3 - Review Southern, Northern & Western blotting DNA sequencing Polymerase chain reaction (PCR) DNA microarrays

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20427%20&%20527/PBIO427%20Lecture%203%202009.ppt

Date added: May 4, 2013 - Views: 7

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PowerPoint Presentation - Slide 1

Chapter 20: Biotechnology

http://www.freewebs.com/nterhune/chapter%2020.ppt

Date added: March 7, 2014 - Views: 1

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Microbiology - Hillsborough Community College

... Dichotomous Key Building a Cladogram Building a Cladogram Building a Cladogram What is tested in Western blotting and Southern blotting? ... Practice ANIMATION Dichotomous Keys: Sample with Flowchart ANIMATION Dichotomous Keys: ...

http://www.hccfl.edu/media/700873/_start-here_ch10_lecture.ppt

Date added: May 3, 2013 - Views: 47

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PowerPoint Presentation

Figure 11.2 Agarose gel electrophoresis of DNA. Figure 11.4 Southern blotting. Figure 11.5 Major steps in gene cloning. ... gene libraries made by cloning random genome fragments © 2012 Pearson Education, Inc. Animation: ...

http://biology.illinoisstate.edu/wanicho/bsc260/ppt/genetic%20engineering.ppt

Date added: October 17, 2014 - Views: 1

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Restriction Fragment Length Polymorphism

Restriction Fragment Length Polymorphism Definition The variation in the length of DNA fragments produced by a restriction endonuclease that cuts at a polymorphic locus.

http://xa.yimg.com/kq/groups/18730409/1826348150/name/Restriction

Date added: May 4, 2013 - Views: 19

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PowerPoint Presentation

Southern blotting: Transfer DNA from ... Southern Blot Animation http://www.sanger.ac.uk/Info/Intro/gfx/fred_bw.jpg Chain termination resulting in different DNA fragment lengths Separate different DNA lengths by gel electrophoresis, ...

http://fmmh.ycdsb.ca/teachers/fmmh_dsilva/pages/SBI%203U7/FOV1-00072661/4.4%20part%203.ppt

Date added: May 20, 2014 - Views: 1

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PowerPoint Presentation

Other Applications of DNA Fingerprinting Parentage test Endangered species or Chinese herbs identification Animation 1: Southern Blotting http://www.dnalc.org/resources/BiologyAnimationLibrary.htm Animation 2: ...

http://www.chuenyuen.edu.hk/~cyc-bio/docs/AL/Electrophoresis%20by%20CUHK.ppt

Date added: March 16, 2012 - Views: 19

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Chapter 12 DNA and RNA

... differences in these sequences can be used to distinguish individuals sequences are separated by gel electrophoresis Southern blotting transfers separated fragments to nylon membrane Hybridization ... Protein Synthesis Animation Biostudio Protein Synthesis Animation Polymerase Chain ...

http://mr-ippolito-science-class.org/files/F_Chapter9DNATheIndispensibleForensicTool200809.pps

Date added: November 29, 2013 - Views: 4

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video slide - Morgan Community College

... of DNA molecules Such analysis can rapidly provide comparative information about DNA sequences Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel ... Recombinant DNA molecule Sticky end Animation: Restriction Enzymes ...

http://www.morgancc.edu/faculty/Smith,L/Biology/Power%20Points/Biology%20Ch%2018/20_Lectures_PPT/20_Lectures_PPT%20.ppt

Date added: May 13, 2013 - Views: 3

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CHAPTER 8 Recombinant DNA Technology

Southern blotting c. Northern blotting Restriction Mapping Animation: Restriction Mapping 1. Cloned DNA can be cut with restriction enzymes and electrophoresed on agarose gels and visualized with ethidium bromide, in order to map its restriction sites (Figure 8.13). 2.

http://grassland.agron.ntu.edu.tw/genelab/Class_slide/Peter_Russell/2Ed/Ch8.ppt

Date added: June 5, 2012 - Views: 30

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Microbiology - Imperial Valley College

Chapter 9 Biotechnology and Recombinant DNA

http://faculty.imperial.edu/samuel.david/bio-220/item/download/56

Date added: November 27, 2013 - Views: 7

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Microbiology - Des Moines Area Community College

... 9-16 Applications of rDNA 9-17 Diagram the Southern blotting ... filter DNA transferred to filter Gel Figure 9.16 Southern blotting. Forensic Microbiology PCR Primer for a specific organism will cause ... Sequence DNA Diagnose genetic disease Detect pathogens ANIMATION ...

http://www.dmacc.edu/instructors/ashelgeson/_ch_09_lecture_presentation.ppt

Date added: May 2, 2013 - Views: 32

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PowerPoint Presentation

Chapter 19 Microbial Models: The Genetics of Viruses and Bacteria

http://faculty.muhs.edu/klestinski/18-19-20.ppt

Date added: February 5, 2014 - Views: 10

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PowerPoint Presentation

Southern blotting, DNA chips, and. FISH . Nucleic Acid Hybridization: DNA Chip. Fig 10.17. Fluorescent In Situ ... Add DNA or RNA probe attached to fluorescent dye. for S. aureus. Dichotomous Key. ANIMATION Dichotomous Keys: Practice. ANIMATION Dichotomous Keys: Sample with Flowchart. ANIMATION ...

http://www.londonderry.org/assets/twps/pattylawson/M_T_Ch10_Classification_SS10.pptx

Date added: October 15, 2014 - Views: 1

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Definition of a Gene - Kaikoura High School

Biotechnology (some definitions) Biotechnology is the development of products using a living organisms to meet a human need or demand. Note that this includes traditional processes such as wine and cheese production as well as more modern technologies.

http://students.kaikourahigh.school.nz/biology/13resources/biotechnology.ppt

Date added: September 18, 2013 - Views: 7

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Gel Electrophoresis of DNA - Universitas Gadjah Mada

... Or DNA can be removed from the gel by Southern Blotting References www.biotech.iastate.edu/publication/ppt-presentations Kreuzer, H., Massey, A., ... The gel is then visualized - UV light causes the bands of DNA to fluoresce Animation More……

http://faperta.ugm.ac.id/newbie/download/pak_tar/genetika-molekuler/presentasi/elpho.ppt

Date added: July 6, 2013 - Views: 14

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Welcome [oscar.iitb.ac.in]

Recombinant DNA Technology Recombinant DNA technology which came into existence in the 1970s, allows for genetic manipulation of organisms by incorporating DNA sequences from different sources into a single recombinant molecule.

http://oscar.iitb.ac.in/OSCARPP/Biology/UploadedStoryboards/SBO021_3P0437Recombinant%20DNA%20Technology.ppt

Date added: August 5, 2013 - Views: 5

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Chapter 20

... go to Animation and ... sequence Target sequence Primers New nucleo- tides 3 3 3 3 5 5 5 1 2 3 Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ...

http://fhsapbio.weebly.com/uploads/1/3/5/3/13535409/ap_bio_ch20_lecture_presentation.ppt

Date added: August 20, 2014 - Views: 1

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Biochemical Properties of DNA and The Technology involve them

Biochemical Properties of DNA and The Technology involve them. Gihan E-H Gawish, MSc, PhD. Ass. ... Southern transfer (blotting) and DNA microarray analyses involve hybridization. ... Animation. 1-Endo-2-Exo-Cleave . Middle of Chain.

http://faculty.ksu.edu.sa/2475/Lectures1/4-Biochemical%20Properties%20of%20DNA%20and%20The%20Technology%20involve%20them.pptx

Date added: February 5, 2012 - Views: 51

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AP Biology Ch. 20 - PBworks

... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting Gel electrophoresis uses a gel as a molecular sieve to separate nucleic acids by size A current is applied to the ... go to Animation and Video Files ...

http://apbiologyclass.pbworks.com/f/AP+Bio+Ch.+20.ppt

Date added: March 14, 2012 - Views: 39

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Rad51-deficient vertebrate cells accumulate chromosomal ...

Animation file made by Justin H. Lo may help you understand how replication is related with homologous recombination: ... (Southern blotting). (A) ...

http://openwetware.org/images/d/d4/Manuscript_Discussion.ppt

Date added: June 2, 2013 - Views: 5

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Biotechnology and Genetic Engineering-PBIO 450/550

See Chapter 9 animation http://bcs.whfreeman.com/lodish7e/#800911__811944__ Fig. 9.3 ... saliva, bone or any other tissue (often found at a crime scene) Can be done by southern blotting with an appropriate probe or by a PCR method using appropriate primers Can use single locus probes ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO4500%20Molecular%20Diagnostics.ppt

Date added: January 21, 2013 - Views: 41

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DNA Technology

... gels for DNA PAGE for proteins Single digest uses 1 enzyme Double digest produces 2 restriction patterns Interactive Animation, Simplified Blotting Goes from the gel to filter paper to make and autoradiograph Southern blot: uses RNA to probe DNA strands (SB2) ...

http://www.africangreyparrott.com/DNATechnology.ppt

Date added: March 4, 2012 - Views: 46

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Chapter 20

... producing fragments with “sticky ends” that bond with complementary sticky ends of other fragments Animation: ... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly ...

http://wildaboutbio.com/uploads/3/1/3/1/3131725/20_lecture_presentation.ppt

Date added: October 21, 2014 - Views: 1

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How Genes and Genomes Evolve

... electrophoretically separated DNA can be screened for fragments of interest using hybridization techniques Northern and Southern blotting are two of ... you can collect your normal and cancer cells and extract the gene expression products – mRNA Animation Denaturation (94°C ...

http://www.as.wvu.edu/~dray/219files/BIO219_Chapter_10_11-9-05_DAR.ppt

Date added: August 5, 2013 - Views: 18