SOUTHERN BLOTTING The technique was developed by E.M. Southern in 1975. ... Would you watch the animation? A. Yes B. No SUMMARY OF PROCEDURE 1. Extract and purify DNA from cells 2. DNA is restricted with enzymes 3. Sort by electrophoresis 4. Denature DNA 5. Transfer to nitrocellulose paper 6.
Date added: June 25, 2012 - Views: 165
Southern Blotting A method to visualize specific segments of DNA– usually a ... with radioactive probe for specific segment Wash away unbound probe Detect probes using x-ray film autoradiograph RFLP Animations Animation #1 Animation #2 Polymerase Chain Reaction PCR allows scientists ...
Date added: April 15, 2012 - Views: 310
Introduction to Southern Hybridization Michael Melzer Plant & Environmental Protection Sciences University of Hawaii at Manoa Outline History/Background Info Goals of Southern hybridization Example Other applications History/Background ‘Southern’ hybridization named after Sir Edwin Southern ...
Date added: November 6, 2011 - Views: 218
Southern Blotting INVENTED BY BRITISH BIOCHEMIST EDWIN SOUTHERN * Purpose Used to determine whether a particular nucleotide sequence is present in different sources of DNA, and also to discover the size of the restriction fragment that contains the specific sequence Example Sickle Cell Can ...
Date added: November 28, 2011 - Views: 238
Size determined by gel electrophoresis and Southern blotting to produce pattern of ... Activities Review tutorial on Western Blot http://tinyurl.com/awaut3 Review VNTR animation http://tinyurl.com/dawjau Review DNA fingerprinting http://tinyurl.com/co2yml Review DNA forensics Problem set 1 ...
Date added: September 13, 2011 - Views: 167
... DNA cloning yields multiple copies of a gene or other DNA ... allele Probe base-pairs with fragments Nitrocellulose blot 4 5 Radioactively labeled probe for -globin gene Southern blotting animation Northern blotting animation Fig. 20-13 TECHNIQUE RESULTS Gel electrophoresis ...
Date added: September 4, 2011 - Views: 109
After gel electrophoresis Filter paper blots DNA off Radioactive probes added Autoradiography pinpoints sequence Southern Blot animation The scientific ... DNA fingerprinting Let’s take a brief look at some of these… What is gel electrophoresis? What is Southern blotting? The ...
Date added: May 22, 2013 - Views: 23
Scene: Animation of labeled probe fragment pairing with target ... Animation of DNA/RNA probe preparations Animation of Complementary Base-Pairing Live shot of Researcher--Segue to Blotting Southern Blot Transfer Preparation Animation of Transfer Process Crosslink of DNA to Nitrocellulose ...
Date added: November 7, 2011 - Views: 31
Steps in Southern Blotting Southern Blotting Animation Advantages and Disadvantages of Southern Blotting Advantages The exact location of a disorder does not need to be known Disadvantages They are rather time consuming Needs high quality/quantity DNA Radioactive Some tasks previously ...
Date added: August 13, 2014 - Views: 1
Scientific Applications Understanding DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern Blotting Southern Blotting Forensic Microbiology PCR Primer for a specific ... Recombinant DNA Technology Table 9.1 Figure 9.2 Figure 9.3 ANIMATION PCR: ...
Date added: October 26, 2011 - Views: 86
... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ... Base pairing occurs. 2 Animation: ...
Date added: May 13, 2013 - Views: 19
This would take about 3 hours to perform in lab. PCR Animation DNA Fingerprinting Used to identify individuals by their repeat regions (usually STR) regions: ... (Southern blotting) A radioactive primer is designed that will be complementary to unique regions (STR, etc, regions).
Date added: February 13, 2013 - Views: 37
Explain how serological tests and phage typing can be used to identify ... Examples of Applications: Southern blotting, DNA chips, and FISH Nucleic Acid Hybridization ... Fig 10.18a–b Add DNA or RNA probe attached to fluorescent dye for S. aureus Dichotomous Key ANIMATION ...
Date added: December 5, 2011 - Views: 79
Polymerase Chain Reaction Animation PCR http://www.dnalc.org/ddnalc/resources/pcr.html Gel electrophoresis a method that ... http://126.96.36.199/pub/flash/4/4.html `http://www.dnalc.org/ddnalc/resources/electrophoresis.html Southern blotting Southern blotting was named after Edward M ...
Date added: March 22, 2012 - Views: 35
* * Figure 20.11 Research Method: Southern Blotting of DNA Fragments * * Figure 20.12 Research Method: ... The fragments are mixed, and DNA ligase is added to bond the fragment sticky ends © 2011 Pearson Education, Inc. Animation: ...
Date added: March 16, 2013 - Views: 21
For the Discovery Video Transgenics, go to Animation and Video Files. ... is used to analyze nucleic acids and to distinguish between two alleles of a gene Describe and distinguish between the Southern blotting procedure, Northern blotting procedure, ...
Date added: June 28, 2013 - Views: 4
Figure 20.10 Restriction fragment analysis by Southern blotting Steps 2 & 3. How to Insert the Gene of Interest into the R-Plasmid See next 3 figures and animation Lyse bacteria with detergent to release the R-plasmid (e.g ...
Date added: October 3, 2014 - Views: 1
Hybridization DNA hybridization Southern blotting (pg75) Southern Blot ... Primer extension Fig. 3.8 The Polymerase Chain Reaction Go to Animation courtesy of the following websites: References http://faculty.plattsburgh.edu/donald.slish/PCRmov.html ...
Date added: July 1, 2012 - Views: 22
Describe how microorganisms can be identified by nucleic acid hybridization, Southern blotting ... taxon, and phylogeny. List the characteristics of the Bacteria, Archaea, and Eukarya domains. Differentiate among eukaryotic, prokaryotic, and ... and FISH Figs. 10.17 and 10.18 PLAY Animation ...
Date added: June 12, 2012 - Views: 68
Several techniques are used to analyze the DNA of genes: Gel Electrophoresis, restriction fragment analysis, Southern blotting, DNA sequencing. ... Figure 20.18 Reproductive cloning of a mammal by nuclear transplantation For the Discovery Video Cloning, go to Animation and Video Files.
Date added: February 9, 2012 - Views: 44
Chapter 20 Biotechnology * Figure 20.9 Gel electrophoresis * Figure 20.9 Gel electrophoresis * Figure 20.9 Gel electrophoresis * * Figure 20.10 Using restriction fragment analysis to distinguish the normal and sickle-cell alleles of the β-globin gene * * Figure 20.11 Southern blotting of DNA ...
Date added: May 3, 2014 - Views: 12
Web animation of a cDNA microarray experiment Yeast genome on a chip Brief outline of steps for ... (e.g. by X-ray for radioactive probes) Sample Autoradiogragh (Gel) Types of Blots Southern ... 3’ 3’ – CTTAAG – 5’ (cut) The Southern blotting technique * Title ...
Date added: October 15, 2011 - Views: 98
PBIO 427/527: Molecular Genetics Lecture 3 - Review Southern, Northern & Western blotting DNA sequencing Polymerase chain reaction (PCR) DNA microarrays
Date added: May 4, 2013 - Views: 7
... Dichotomous Key Building a Cladogram Building a Cladogram Building a Cladogram What is tested in Western blotting and Southern blotting? ... Practice ANIMATION Dichotomous Keys: Sample with Flowchart ANIMATION Dichotomous Keys: ...
Date added: May 3, 2013 - Views: 47
Figure 11.2 Agarose gel electrophoresis of DNA. Figure 11.4 Southern blotting. Figure 11.5 Major steps in gene cloning. ... gene libraries made by cloning random genome fragments © 2012 Pearson Education, Inc. Animation: ...
Date added: October 17, 2014 - Views: 1
Restriction Fragment Length Polymorphism Definition The variation in the length of DNA fragments produced by a restriction endonuclease that cuts at a polymorphic locus.
Date added: May 4, 2013 - Views: 19
Southern blotting: Transfer DNA from ... Southern Blot Animation http://www.sanger.ac.uk/Info/Intro/gfx/fred_bw.jpg Chain termination resulting in different DNA fragment lengths Separate different DNA lengths by gel electrophoresis, ...
Date added: May 20, 2014 - Views: 1
Other Applications of DNA Fingerprinting Parentage test Endangered species or Chinese herbs identification Animation 1: Southern Blotting http://www.dnalc.org/resources/BiologyAnimationLibrary.htm Animation 2: ...
Date added: March 16, 2012 - Views: 19
... differences in these sequences can be used to distinguish individuals sequences are separated by gel electrophoresis Southern blotting transfers separated fragments to nylon membrane Hybridization ... Protein Synthesis Animation Biostudio Protein Synthesis Animation Polymerase Chain ...
Date added: November 29, 2013 - Views: 4
... of DNA molecules Such analysis can rapidly provide comparative information about DNA sequences Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel ... Recombinant DNA molecule Sticky end Animation: Restriction Enzymes ...
Date added: May 13, 2013 - Views: 3
Southern blotting c. Northern blotting Restriction Mapping Animation: Restriction Mapping 1. Cloned DNA can be cut with restriction enzymes and electrophoresed on agarose gels and visualized with ethidium bromide, in order to map its restriction sites (Figure 8.13). 2.
Date added: June 5, 2012 - Views: 30
... 9-16 Applications of rDNA 9-17 Diagram the Southern blotting ... filter DNA transferred to filter Gel Figure 9.16 Southern blotting. Forensic Microbiology PCR Primer for a specific organism will cause ... Sequence DNA Diagnose genetic disease Detect pathogens ANIMATION ...
Date added: May 2, 2013 - Views: 32
Southern blotting, DNA chips, and. FISH . Nucleic Acid Hybridization: DNA Chip. Fig 10.17. Fluorescent In Situ ... Add DNA or RNA probe attached to fluorescent dye. for S. aureus. Dichotomous Key. ANIMATION Dichotomous Keys: Practice. ANIMATION Dichotomous Keys: Sample with Flowchart. ANIMATION ...
Date added: October 15, 2014 - Views: 1
Biotechnology (some definitions) Biotechnology is the development of products using a living organisms to meet a human need or demand. Note that this includes traditional processes such as wine and cheese production as well as more modern technologies.
Date added: September 18, 2013 - Views: 7
... Or DNA can be removed from the gel by Southern Blotting References www.biotech.iastate.edu/publication/ppt-presentations Kreuzer, H., Massey, A., ... The gel is then visualized - UV light causes the bands of DNA to fluoresce Animation More……
Date added: July 6, 2013 - Views: 14
Recombinant DNA Technology Recombinant DNA technology which came into existence in the 1970s, allows for genetic manipulation of organisms by incorporating DNA sequences from different sources into a single recombinant molecule.
Date added: August 5, 2013 - Views: 5
... go to Animation and ... sequence Target sequence Primers New nucleo- tides 3 3 3 3 5 5 5 1 2 3 Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ...
Date added: August 20, 2014 - Views: 1
Biochemical Properties of DNA and The Technology involve them. Gihan E-H Gawish, MSc, PhD. Ass. ... Southern transfer (blotting) and DNA microarray analyses involve hybridization. ... Animation. 1-Endo-2-Exo-Cleave . Middle of Chain.
Date added: February 5, 2012 - Views: 51
... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting Gel electrophoresis uses a gel as a molecular sieve to separate nucleic acids by size A current is applied to the ... go to Animation and Video Files ...
Date added: March 14, 2012 - Views: 39
Animation file made by Justin H. Lo may help you understand how replication is related with homologous recombination: ... (Southern blotting). (A) ...
Date added: June 2, 2013 - Views: 5
See Chapter 9 animation http://bcs.whfreeman.com/lodish7e/#800911__811944__ Fig. 9.3 ... saliva, bone or any other tissue (often found at a crime scene) Can be done by southern blotting with an appropriate probe or by a PCR method using appropriate primers Can use single locus probes ...
Date added: January 21, 2013 - Views: 41
... gels for DNA PAGE for proteins Single digest uses 1 enzyme Double digest produces 2 restriction patterns Interactive Animation, Simplified Blotting Goes from the gel to filter paper to make and autoradiograph Southern blot: uses RNA to probe DNA strands (SB2) ...
Date added: March 4, 2012 - Views: 46
... producing fragments with “sticky ends” that bond with complementary sticky ends of other fragments Animation: ... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly ...
Date added: October 21, 2014 - Views: 1
... electrophoretically separated DNA can be screened for fragments of interest using hybridization techniques Northern and Southern blotting are two of ... you can collect your normal and cancer cells and extract the gene expression products – mRNA Animation Denaturation (94°C ...
Date added: August 5, 2013 - Views: 18