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Cloning into Plasmids - Buffalo State College...

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Cloning into Plasmids - Buffalo State College...

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 95

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. ... Cosmids. B. Vectors for Other Organisms. 1. Yeast Artificial Chromosomes ...

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 167

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Slide 1

PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202010%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: October 7, 2011 - Views: 426

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Cloning - Sam Houston State University

Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 27

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 3

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Slide 1

Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 1

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No Slide Title

... “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, ...

http://www.bio.utk.edu/humangenetics/lecture/molec_tools06sep02.ppt

Date added: March 7, 2015 - Views: 1

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 4

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 27

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PowerPoint Presentation

Lambda Bacteriophage Vectors cos cos cos Selection: Only recombinant phage produce plaques. 1. Size ... Cosmids: Plasmids that contain l cos sites.

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: January 4, 2015 - Views: 1

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Introduction to molecular cloning - ABE

Plasmids – used in cloning ... Cosmidsplasmids ... small circular dsDNA that autonomously replicates apart from the chromosome of the host cell “molecular ...

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 86

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Inquiry into Life Twelfth Edition - Regis...

vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 32

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 76

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PowerPoint Presentation

Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 14

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PowerPoint Presentation

Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 21

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Viral Vectors - University of Saskatchewan

Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: February 19, 2012 - Views: 39

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Foundations in Microbiology

Chapter 10 Genetic Engineering: A Revolution in Molecular Biology

http://learning.hccs.edu/faculty/harold.njemanze/micro2420-1/micro-powerpoint/chapter-10cr

Date added: April 1, 2015 - Views: 1

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Gene cloning tutorial - University of Leicester

Cosmids can be used to clone inserts ... Both of these vectors are cloning vectors and not expression vectors and ... Yeast plasmids usually carry an ...

http://www2.le.ac.uk/departments/genetics/vgec/downloads/educators-over-18s/Gene%20Cloning/2%20CloningPowerPoint.pptx

Date added: June 10, 2013 - Views: 43

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基因重組原理與技術

... (Cloning Vectors) 細菌 – 質體 (plasmids) 噬菌體 (bacteriophages) 黏接質體 ... (Cloning Vectors) 噬菌體與 cosmids 舉例 轉殖載體 ...

http://elearning.ice.ntnu.edu.tw/km/Data/Teacher/31972/data/%E6%88%91%E7%9A%84%E5%80%8B%E4%BA%BA%E6%96%87%E4%BB%B6/lecture%202%20-%20%E5%9F%BA%E5%9B%A0%E9%87%8D%E7%B5%84%E5%8E%9F%E7%90%86%E8%88%87%E6%8A%80%E8%A1%93-eef23.ppt

Date added: March 29, 2012 - Views: 2

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الشريحة 1 - Majmaah University

Plasmids. Bacteriophages. Cosmids: ... Molecular cloning vectors. Gene cloning. Insulin production by gene cloning. BEST WISHES. Author: Emad A. Morad Created Date:

http://faculty.mu.edu.sa/download.php?fid=78185

Date added: March 1, 2014 - Views: 2

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PowerPoint 演示文稿

... Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors ... libraries from prokaryotes in plasmids where the insert size is 5-10kb ...

http://202.114.65.51/fzjx/fzswx/wlkt/ppt2005/application1.ppt

Date added: March 2, 2012 - Views: 9

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Section D - Prokaryotic and Eukaryotic Chromosome...

Section G – Gene manipulation Contents G1 DNA cloning: an overview DNA cloning, Hosts and vectors, Subcloning, DNA libraries, Screening libraries, Analysis of a ...

http://fzswx.gxu.edu.cn/course/Section%20G%20-%20Gene%20manipulation.ppt

Date added: November 27, 2013 - Views: 11

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Chpter 4 - 建構中

Chapter 4 Molecular Cloning Methods Gene Cloning The Role of Restriction Endonuclease Vectors Plasmids : carriers ... Cosmids * cohesive ends of l phage DNA ...

http://memo.cgu.edu.tw/jau-song/slide/G/Molecular%20%20Biology%20%20Chpter%204.ppt

Date added: October 29, 2012 - Views: 21

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Plasmids and Vectors - Molecular Biology for...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: January 31, 2015 - Views: 2

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PowerPoint 프레젠테이션

Common vectors used in libraries Plasmids Up to 10kbp inserts Modified Lambda phage Up to 20kbp inserts/40kbp for cosmids Artificial cloning vectors BAC- Vectors ...

http://ani.gntech.ac.kr/html/data/genetics%20engneering-ch5.ppt

Date added: February 8, 2014 - Views: 13

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Vectors - College of Computer, Mathematical, and...

Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: September 11, 2011 - Views: 266

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ai.stanford.edu

ai.stanford.edu

http://ai.stanford.edu/~serafim/CS262_2009/presentations/lecture9.pptx

Date added: February 8, 2015 - Views: 3

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Chpter 4

Chapter 4 Molecular Cloning Methods

http://memo.cgu.edu.tw/tzu-chien/MB2009/Yu2009-1.ppt

Date added: May 10, 2013 - Views: 20

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第六章 - 同济大学医学院

Vectors: plasmid, λphage, ... Plasmids usually occur naturally in bacteria, ... Cosmids can be used to build genomic library.

http://med.tongji.edu.cn/yzkc/DT_UploadFile/20101224183754546.ppt

Date added: June 13, 2012 - Views: 21

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Lambda Vectors and their replication - Hunter...

Lambda vectors and their ... Plasmids- small circular DNA molecules which can replicate their DNA independently ... Cosmids- are hybrid vectors of l phage and ...

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 41

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 227

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PowerPoint Presentation

Gene expression * Expression vectors: ... E. coli cloning vector (circular): plasmids (质粒 ... plasmid-bacteriophage l hybrids (cosmids) (考斯质粒 ...

http://202.114.65.51/fzjx/fzswx/wlkt/ppt2008/2008chapter21.ppt

Date added: November 11, 2011 - Views: 13

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 20

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Restriction Enzymes, Vectors, and Genetic...

... Linear DNA Can insert up to 15 kb fragments Phage Insertion Cosmids Don’t ... Eco R1 Vectors A self-replicating DNA ... of Vectors Plasmids ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 33

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Chapter 4 Molecular Cloning Methods - Xiamen...

Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 172

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Section D - Prokaryotic and Eukaryotic Chromosome...

H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 15

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Slide 1

... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...

http://classes.biology.ucsd.edu/old.web.classes/bimm120.WI06/john_buchanan_lec2.ppt

Date added: September 15, 2012 - Views: 20

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Slide 1

... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, ... color change Plasmids vectors Easy to isolate and ...

http://classes.biology.ucsd.edu/bimm120.WI07/genetics2_doran_fa06.ppt

Date added: September 7, 2012 - Views: 31

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_3.ppt

Date added: July 1, 2014 - Views: 6

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PowerPoint Presentation

Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures/chapter_08.ppt

Date added: April 10, 2015 - Views: 1

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Chapter 4

Lecture 5 Recombinant DNA Technology Cloning Vectors Gene Libraries Clone Identification and Characterization Reading: Chapter 9 Molecular Biology syllabus web site

http://a32.lehman.cuny.edu/webwurtzel/course/Graduate%20lectures/lecture%205/lecture%205_Recombinant%20DNA%20Techniques.pps

Date added: January 27, 2012 - Views: 28

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Recombinant DNA Technology - NIU Department of...

Recombinant DNA Technology ... Plasmids for cloning carry drug resistance genes ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ...

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 262

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Trends in Biotechnology - hallym.ac.kr

Trends in Biotechnology 110509 3b – Vectors

http://web.hallym.ac.kr/~colinbrowne/TrendsBiotechnology_files/110509%203b.ppt

Date added: December 5, 2013 - Views: 10

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Setting up a transformation--how will the...

... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: November 15, 2011 - Views: 76

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Inquiry into Life Twelfth Edition - McGraw-Hill...

Inquiry into Life Twelfth Edition ... Vectors Plasmids As ... via Plaque Hybridization Cosmids M13 Phage Vectors M13 Cloning to Recover ...

http://highered.mheducation.com/sites/dl/free/0073525324/891545/Chapter_04_lecture.ppt

Date added: July 10, 2014 - Views: 4

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Slide 1

The original plate is used to pick bacterial cells with recombinant plasmids that hybridized ... Plasmids Phages Cosmids New generation vectors: Bacterial ...

http://higheredbcs.wiley.com/legacy/college/allison/1118059816/ppt/ch08.ppt

Date added: August 2, 2013 - Views: 24

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Slide 1

Chapter 13: Biotechnology

http://facstaff.gpc.edu/~jaustin/images/chapterthirteen.ppt

Date added: May 15, 2013 - Views: 2

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Genetic Engineering

Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase Splice into Cloning Vehicle Plasmids ... Cosmids Other Vectors ...

p://people.uncw.edu/sizemorer

Date added: November 5, 2014 - Views: 32

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Inquiry into Life Twelfth Edition - McGraw-Hill...

... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 35