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Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs (Bacterial artificial chromosomes) Large low copy number plasmids (have ori and ...
Date added: October 16, 2011 - Views: 192
Cosmids are extracted from bacteria ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids Phage BACs and YACs HIV Gene Delivery System Summary References http://www.rvc.ac.uk/Extranet/DNA_1/DNA_1_intro ...
Date added: July 18, 2013 - Views: 37
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 210
Cosmids- are hybrid vectors of l phage and plasmids. They can replicate their DNA in the cell with a plasmid and be packaged like a phage. ... Cosmids- are hybrid vectors of l phage and plasmids. They can replicate their DNA in the cell with a plasmid and be packaged like a phage.
Date added: September 30, 2013 - Views: 31
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the cloning of DNA.
Date added: December 2, 2011 - Views: 92
... the gene from the rest DNA 4.1 Gene Cloning The Role of Restriction Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors Phagemids Eukaryotic Vectors Identifying a Specific Clone with a Specific Probe Polynucleotide ...
Date added: January 28, 2012 - Views: 161
3 Basic Principles of Recombinant DNA Technology. ... DNA Cloning. V. Cloning Vectors. A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. 3. Cosmids. B. Vectors for Other Organisms. 1. Yeast Artificial Chromosomes (YACs) 2. Bacterial Artificial Chromosomes (BACs) 3. Plant ...
Date added: May 26, 2012 - Views: 141
... Linear DNA Can insert up to 15 kb fragments Phage Insertion Cosmids Don’t occur naturally Constructed using features of both plasmids and phage Can carry ... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors ...
Date added: May 3, 2013 - Views: 22
Vectors contain selectable markers and replication origins to allow identification and maintenance of the vector in the host. ... is a cloning vector 3.4 Cloning Vectors Can Be Specialized for Different Purposes Cloning vectors may be bacterial plasmids, phages, cosmids, ...
Date added: October 23, 2013 - Views: 8
Plasmid vectors containing the lambda cos sites are called cosmids, ... Plasmids as Cloning Vectors Plasmids are useful cloning vectors (Figure 10.36) because they are easy to isolate and purify and can multiply to high copy numbers in bacterial cells.
Date added: May 29, 2013 - Views: 23
Plasmids and other types of cloning vectors were used by Human Genome Project researchers to copy genes and other pieces of chromosomes to generate enough identical ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of foreign DNA and can be packaged in lambda phage ...
Date added: August 27, 2013 - Views: 16
Cloning Vectors Recombinant DNA ... 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) - based on bacterial mini-F plasmids. cloning limit: 150-300 kb ...
Date added: February 18, 2014 - Views: 9
Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
Date added: May 2, 2013 - Views: 19
Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication, determines the number of copies per cell Marker genes: ampicillin and tetracycline resistance genes Unique restriction enzyme cut ...
Date added: May 6, 2013 - Views: 2
H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes in Saccharomyces cerevisiae. Based on 2 micron (2m) ... H2 Bacteriophage vectors — Cloning in M13 H2 Bacteriophage vectors — Hybrid plasmid-M13 vectors H3 Cosmids, ...
Date added: February 13, 2012 - Views: 10
... F plasmid-based Cosmids: 5 kb plasmids, ... Overview of strategies for cloning genes * Mobilizing DNA: vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression vectors and tags vectors for large pieces of DNA, ...
Date added: November 15, 2011 - Views: 66
... Plasmid cloning vector pBR322, Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids phasmids Phagemids BAC vectors PAC vectors YAC vectors Model vectors for eukaryotes ... Phagemid Vectors Contain components from phage chromosomes and plasmids.
Date added: July 1, 2014 - Views: 3
Cosmids combine phage vectors’ ability to be packaged with the DNA propagation and purification properties of plasmids. ... to prevent degradation by cellular DNases.Picture Cosmid features and uses Cosmids are predominantly plasmids with a bacterial oriV, ...
Date added: May 21, 2012 - Views: 49
... Restriction enzymes - cleave at specific sites, palindromes, “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...
Date added: November 28, 2013 - Views: 3
Lecture 5 Recombinant DNA Technology Cloning Vectors Gene Libraries Clone Identification and Characterization Reading: Chapter 9 Molecular Biology syllabus web site
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Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2. Bacteriophage: Small viruses that infect ... Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b ...
Date added: May 13, 2013 - Views: 21
Recombinant DNA Technology ... Plasmids: independently replicating DNA circles (only circles replicate in bacteria). Foreign DNA can be inserted into a plasmid ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: use lambda, but remove all but the ends (cos ...
Date added: October 7, 2011 - Views: 250
Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) from bacteriophage λ used to clone larger fragments of up to 45 Kb Bacteriophage λ ... Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date:
Date added: November 5, 2011 - Views: 80
... (methylated) at the corresponding sequences by a methylase. 2. Plasmids: independently ... Most cloning vectors have a multiple cloning site, a ... I hope Cloning Vector Types For different sizes of DNA: plasmids: up to 5 kb phage lambda (λ) vectors (also cosmids): up to 50 kb ...
Date added: May 8, 2012 - Views: 53
... bacteriophage lambda used as the vector “Stuffer fragment” removed and replaced with 15-17kbp fragments of library Cosmids and YACs may also be used as vectors Contains at least one ... A plasmid vector for cloning Plasmid vectors Examples pBR322 One of the original plasmids ...
Date added: May 2, 2013 - Views: 29
... How to make and screen gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...
Date added: October 6, 2011 - Views: 26
Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more commonly used E. coli plasmid vectors are useful for cloning DNA fragments up to ... recombinant plasmids containing inserted fragments up to 45 kb long can be efficiently introduced into E. coli ...
Date added: October 7, 2011 - Views: 265
... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids As ... -stranded phage DNA is secreted from transformed cells and can be collected from media Phagemids Phagemids are also vectors Like cosmids have aspects of both phages and plasmids ...
Date added: July 14, 2014 - Views: 15
... color change Plasmids vectors Easy to isolate and purify Can be introduced into bacteria by transformation Often bear antibiotic ... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids with lambda ...
Date added: September 15, 2012 - Views: 18
... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, artificial chromosomes ... color change Plasmids vectors Easy to isolate and purify Can be introduced into bacteria by transformation Often bear antibiotic resistance genes that can ...
Date added: September 7, 2012 - Views: 23
... but it can also be 125I Animations for two related uses of expression vectors Expression cloning of receptor proteins-see MCB Chapter 5 http://bcs ... Several different cloning vectors including plasmids ... Complementary DNA or cDNA cloning: cDNA library ...
Date added: February 13, 2013 - Views: 31
Unit I Lecture 4 B. Tech. (Biotechnology) III Year V th Semester EBT-501, Genetic Engineering BACs and PACs are vectors that can carry much larger fragments of DNA than cosmids because they do not have packaging constraints Figure 2.24 Genomes 3 (© Garland Science 2007) The PAC Mammalian ...
Date added: June 18, 2014 - Views: 5
Cloning Vectors Target Gene ... encoded by the Protein X gene Define the Protein X gene structure Construct expression plasmids for functional studies of Protein X in cells Mutagenize the ... Bacterial host. Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules ...
Date added: October 7, 2011 - Views: 106
Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; ... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: ...
Date added: August 2, 2013 - Views: 12
... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids As Vectors ... -stranded phage DNA is secreted from transformed cells and can be collected from media Phagemids Phagemids are also vectors Like cosmids have aspects of both phages and ...
Date added: July 10, 2014 - Views: 1
Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3 ... Slide 5 Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain plasmid Types of Genes No Lactose Present Lactose Present Transcriptional ...
Date added: March 14, 2012 - Views: 68
Chapters 8, 9, 10 - Recombinant DNA Technology and Other Molecular Methods Used in Genomic Analysis Lots of topics to be covered including: Recombinant DNA
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vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. Origin of replication. pBR322. 4-pBR322 Cloning. Clone foreign DNA into . Pst. I site of pBR322. ... Cosmids. Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage.
Date added: August 2, 2013 - Views: 17
Other vectors include bacteriophage, cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation Selection for cells carrying recombinant plasmids by plating cells on media with antibiotic.
Date added: January 13, 2013 - Views: 18
Why Plasmids are Good Cloning Vectors small size (easy to manipulate and isolate) circular (more stable) replication independent of host cell several copies may be present ... Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: June 1, 2013 - Views: 9
Viral Vectors May 8 Viral vectors ... cell-cell spread suppression of class I MHC promoter pA pack HSV-ori bacterial plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined ... POL, ENV - required for replication clone gene of interest plasmids expressing ...
Date added: February 19, 2012 - Views: 39
... bacteria as the “factory” reproduce incredibly quickly have natural VECTOR Vectors carry the target gene Cloning Vectors segments of DNA that carry target gene; allow reproduction of gene plasmids phages cosmids part viral, ...
Date added: March 10, 2012 - Views: 25
Carriers of foreign DNA are Vectors: Most vectors are derived from: 1. Plasmids 2. ... Plasmids 2. Bacteriophages 3. Cosmids (artificial constructions) A prokaryotic vector should: 1. Be capable of autonomous replication independent of the main bacterial chromosome 2. Be ...
Date added: October 16, 2012 - Views: 24