Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 95
3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. ... Cosmids. B. Vectors for Other Organisms. 1. Yeast Artificial Chromosomes ...
Date added: May 26, 2012 - Views: 167
PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: October 7, 2011 - Views: 426
Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...
Date added: August 27, 2013 - Views: 27
Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...
Date added: May 6, 2013 - Views: 3
Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...
Date added: January 25, 2015 - Views: 1
... “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, ...
Date added: March 7, 2015 - Views: 1
Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...
Date added: August 2, 2013 - Views: 4
Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...
Date added: October 6, 2011 - Views: 27
Lambda Bacteriophage Vectors cos cos cos Selection: Only recombinant phage produce plaques. 1. Size ... Cosmids: Plasmids that contain l cos sites.
Date added: January 4, 2015 - Views: 1
Plasmids – used in cloning ... Cosmids – plasmids ... small circular dsDNA that autonomously replicates apart from the chromosome of the host cell “molecular ...
Date added: November 5, 2011 - Views: 86
vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...
Date added: August 2, 2013 - Views: 32
Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...
Date added: March 14, 2012 - Views: 76
Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...
Date added: August 2, 2013 - Views: 14
Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...
Date added: January 13, 2013 - Views: 21
Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...
Date added: February 19, 2012 - Views: 39
Chapter 10 Genetic Engineering: A Revolution in Molecular Biology
Date added: April 1, 2015 - Views: 1
Cosmids can be used to clone inserts ... Both of these vectors are cloning vectors and not expression vectors and ... Yeast plasmids usually carry an ...
Date added: June 10, 2013 - Views: 43
... (Cloning Vectors) 細菌 – 質體 (plasmids) 噬菌體 (bacteriophages) 黏接質體 ... (Cloning Vectors) 噬菌體與 cosmids 舉例 轉殖載體 ...
Date added: March 29, 2012 - Views: 2
Plasmids. Bacteriophages. Cosmids: ... Molecular cloning vectors. Gene cloning. Insulin production by gene cloning. BEST WISHES. Author: Emad A. Morad Created Date:
Date added: March 1, 2014 - Views: 2
... Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors ... libraries from prokaryotes in plasmids where the insert size is 5-10kb ...
Date added: March 2, 2012 - Views: 9
Section G – Gene manipulation Contents G1 DNA cloning: an overview DNA cloning, Hosts and vectors, Subcloning, DNA libraries, Screening libraries, Analysis of a ...
Date added: November 27, 2013 - Views: 11
Chapter 4 Molecular Cloning Methods Gene Cloning The Role of Restriction Endonuclease Vectors Plasmids : carriers ... Cosmids * cohesive ends of l phage DNA ...
Date added: October 29, 2012 - Views: 21
Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...
Date added: January 31, 2015 - Views: 2
Common vectors used in libraries Plasmids Up to 10kbp inserts Modified Lambda phage Up to 20kbp inserts/40kbp for cosmids Artificial cloning vectors BAC- Vectors ...
Date added: February 8, 2014 - Views: 13
Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...
Date added: September 11, 2011 - Views: 266
Date added: February 8, 2015 - Views: 3
Chapter 4 Molecular Cloning Methods
Date added: May 10, 2013 - Views: 20
Vectors: plasmid, λphage, ... Plasmids usually occur naturally in bacteria, ... Cosmids can be used to build genomic library.
Date added: June 13, 2012 - Views: 21
Lambda vectors and their ... Plasmids- small circular DNA molecules which can replicate their DNA independently ... Cosmids- are hybrid vectors of l phage and ...
Date added: September 30, 2013 - Views: 41
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 227
Gene expression * Expression vectors: ... E. coli cloning vector (circular): plasmids (质粒 ... plasmid-bacteriophage l hybrids (cosmids) (考斯质粒 ...
Date added: November 11, 2011 - Views: 13
Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
Date added: May 2, 2013 - Views: 20
... Linear DNA Can insert up to 15 kb fragments Phage Insertion Cosmids Don’t ... Eco R1 Vectors A self-replicating DNA ... of Vectors Plasmids ...
Date added: May 3, 2013 - Views: 33
Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...
Date added: January 28, 2012 - Views: 172
H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...
Date added: February 13, 2012 - Views: 15
... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...
Date added: September 15, 2012 - Views: 20
... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, ... color change Plasmids vectors Easy to isolate and ...
Date added: September 7, 2012 - Views: 31
... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.
Date added: July 1, 2014 - Views: 6
Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.
Date added: April 10, 2015 - Views: 1
Lecture 5 Recombinant DNA Technology Cloning Vectors Gene Libraries Clone Identification and Characterization Reading: Chapter 9 Molecular Biology syllabus web site
Date added: January 27, 2012 - Views: 28
Recombinant DNA Technology ... Plasmids for cloning carry drug resistance genes ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ...
Date added: October 7, 2011 - Views: 262
Trends in Biotechnology 110509 3b – Vectors
Date added: December 5, 2013 - Views: 10
... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression ...
Date added: November 15, 2011 - Views: 76
Inquiry into Life Twelfth Edition ... Vectors Plasmids As ... via Plaque Hybridization Cosmids M13 Phage Vectors M13 Cloning to Recover ...
Date added: July 10, 2014 - Views: 4
The original plate is used to pick bacterial cells with recombinant plasmids that hybridized ... Plasmids Phages Cosmids New generation vectors: Bacterial ...
Date added: August 2, 2013 - Views: 24
Chapter 13: Biotechnology
Date added: May 15, 2013 - Views: 2
Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase Splice into Cloning Vehicle Plasmids ... Cosmids Other Vectors ...
Date added: November 5, 2014 - Views: 32
... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:
Date added: July 14, 2014 - Views: 35