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Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...
Date added: October 16, 2011 - Views: 199
Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...
Date added: July 18, 2013 - Views: 44
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 223
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 93
Lambda vectors and their ... Plasmids- small circular DNA molecules which can replicate their DNA independently ... Cosmids- are hybrid vectors of l phage and ...
Date added: September 30, 2013 - Views: 35
... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors ... vectors Phage Insertion Cosmids ...
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Chapter 4 Molecular Cloning Methods Introduction The significance of gene cloning To elucidate the structure and function of genes. i.e.: investigating hGH gene hGH ...
Date added: January 28, 2012 - Views: 165
H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...
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3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. ... Cosmids. B. Vectors for Other Organisms. 1. Yeast Artificial Chromosomes ...
Date added: May 26, 2012 - Views: 150
Vectors contain selectable markers ... vectors may be bacterial plasmids, phages, cosmids, ... 3 Methods in Molecular Biology and Genetic Engineering 3.1 ...
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Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
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Cloning Vectors Recombinant DNA ... 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) ...
Date added: February 18, 2014 - Views: 12
Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...
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Setting up a transformation--how will the competent ... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage ...
Date added: November 15, 2011 - Views: 71
Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...
Date added: August 27, 2013 - Views: 19
... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.
Date added: July 1, 2014 - Views: 4
Examples of large-insert cloning vectors are cosmids, ... by cellular DNases.Picture Cosmid features and uses Cosmids are predominantly plasmids with a bacterial ...
Date added: May 21, 2012 - Views: 55
Foreign DNA Host organism Vector DNA for cloning Means of inserting ... Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids ...
Date added: November 5, 2011 - Views: 83
Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.
Date added: October 2, 2014 - Views: 3
Recombinant DNA Technology ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ... The recombinant plasmids are transformed into E. coli ...
Date added: October 7, 2011 - Views: 254
... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids ... are also vectors Like cosmids have aspects of ...
Date added: July 10, 2014 - Views: 2
Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...
Date added: October 6, 2011 - Views: 26
Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more ... Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...
Date added: October 7, 2011 - Views: 281
Library screening Eukaryotic ... How to make and screen gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids ...
Date added: February 13, 2013 - Views: 35
Plasmids for cloning carry drug resistance genes that are used for selection. ... (λ) vectors (also cosmids): up to 50 kb BAC (bacterial artificial chromosome): ...
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... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:
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vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...
Date added: August 2, 2013 - Views: 25
Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...
Date added: August 2, 2013 - Views: 14
Cloning Vectors Target Gene ... structure Construct expression plasmids for functional studies of Protein X in cells Mutagenize ... Bacteriophage-based Cosmids: ...
Date added: October 7, 2011 - Views: 109
... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids phasmids Phagemids BAC ... Yeast episomal plasmids YEps were ...
Date added: June 18, 2014 - Views: 6
... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...
Date added: September 15, 2012 - Views: 19
Exploring Genes Recombinant ... or cohesive ends Cloning Vectors Plasmids and ... Recombinant DNA What are cosmids? combinations of plasmids and lambda phages can ...
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Vectors. Plasmid – 10-20kbp ... Cosmids – a plasmid + Lambda DNA hybrid, 50 kb inserts. Bacterial ... F plasmids that can accept 150-300 kb inserts. Yeast ...
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... VECTOR Vectors carry the target gene Cloning Vectors segments of DNA that carry target gene; allow reproduction of gene plasmids phages cosmids part ...
Date added: March 10, 2012 - Views: 26
Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.
Date added: October 16, 2012 - Views: 28
Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...
Date added: February 19, 2012 - Views: 39