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Plasmids and Vectors - Molecular Biology for Secondary ...

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Plasmids and Vectors - Molecular Biology for Secondary ...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: January 31, 2015 - Views: 1

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Vectors - College of Computer, Mathematical, and Natural Sciences

Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Date added: July 18, 2013 - Views: 44

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 225

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Cloning into Plasmids - Buffalo State College Faculty and ...

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 93

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3 Basic Principles of Recombinant DNA Technology - User Homepages

3 Basic Principles of Recombinant DNA Technology. ... Cosmids. B. Vectors for Other Organisms. ... Engineered Ti plasmids lack the tumor-causing genes, ...

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 159

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Lambda Vectors and their replication - Department Of ...

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 36

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Restriction Enzymes, Vectors, and Genetic Libraries

... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors ... vectors Phage Insertion Cosmids ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 32

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Slide 1

PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202010%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: October 7, 2011 - Views: 423

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Cloning - Sam Houston State University - Huntsville, TX

Plasmids and other types of cloning vectors were used by Human Genome Project researchers to copy ... Cosmids are artificially constructed cloning vectors that ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 21

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Gene Cloning

Cloning Vectors Recombinant DNA ... 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) ...

http://xa.yimg.com/kq/groups/24240295/1734630456/name/lecture+04.ppt

Date added: February 18, 2014 - Views: 12

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 4

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PowerPoint Presentation

Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://mercury2.iab.uaf.edu/kevin_mccracken/genetics/lectures/chapter_08.ppt

Date added: August 31, 2013 - Views: 4

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Genetic Engineering

Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase Splice into Cloning Vehicle Plasmids ... Cosmids Other Vectors ...

p://people.uncw.edu/sizemorer

Date added: November 5, 2014 - Views: 13

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Section D - Prokaryotic and Eukaryotic Chromosome Structure

H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 12

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DNA Recombination Technology

Vectors (cloning vehicles) Enzymes for cutting and joining the DNA fragments. ... Types of Vectors. Plasmids. Cosmids. Fosmids. Phages. Yeast Artificial Chromosomes ...

http://karofarmasirolas.files.wordpress.com/2013/04/cloning-vector.pptx

Date added: January 22, 2015 - Views: 1

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PowerPoint Presentation

Examples of large-insert cloning vectors are cosmids, ... by cellular DNases.Picture Cosmid features and uses Cosmids are predominantly plasmids with a bacterial ...

http://forest.mtu.edu/classes/fw3300/BL3300%202011/2011%20%20Chapt%2003%20091904%20cpj.ppt

Date added: May 21, 2012 - Views: 57

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Setting up a transformation--how will the competent cells be ...

... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: November 15, 2011 - Views: 72

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Molecular Cloning - Advances in Bioscience Education (ABE)

Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmidsplasmids containing DNA sequences (cos) ... Molecular Cloning Author: Lab

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 83

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 20

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Recombinant DNA Technology - NIU Department of Biological ...

Plasmids: independently ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ... Recombinant DNA Technology Needles in Haystacks How to ...

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 257

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Chapter 4

Lecture 5 Recombinant DNA Technology Cloning Vectors Gene Libraries Clone Identification and Characterization Reading: Chapter 9 Molecular Biology syllabus web site

http://a32.lehman.cuny.edu/webwurtzel/course/Graduate%20lectures/lecture%205/lecture%205_Recombinant%20DNA%20Techniques.pps

Date added: January 27, 2012 - Views: 25

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Chapter 4 Molecular Cloning Methods

Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 169

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Plasmid DNA isolation

Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 1

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Inquiry into Life Twelfth Edition - Innovative Learning ...

... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids ... are also vectors Like cosmids have aspects of ...

http://highered.mheducation.com/sites/dl/free/0073525324/891545/Chapter_04_lecture.ppt

Date added: July 10, 2014 - Views: 2

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PowerPoint Presentation

Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 14

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 27

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Part II: Recombinant DNA Technology.ppt - Rajesh Banu

... vector Size of library depends on abundance of message Bacteriophage lambda insertion vectors or plasmids are used for ... can be accommodated by cosmids ...

http://rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: May 2, 2013 - Views: 51

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules ... Examples: Cosmids PACs BACs Plasmid Vectors ... Allows you to select bacteria that have plasmids with an ...

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 26

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 4500 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO4500%20Lecture%203.ppt

Date added: January 27, 2013 - Views: 13

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Slide 1

... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, ... color change Plasmids vectors Easy to isolate and ...

http://classes.biology.ucsd.edu/bimm120.WI07/genetics2_doran_fa06.ppt

Date added: September 7, 2012 - Views: 30

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_3.ppt

Date added: July 1, 2014 - Views: 4

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Inquiry into Life Twelfth Edition - Innovative Learning ...

... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 28

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Recombinant DNA Technology

Plasmids for cloning carry drug resistance genes that are used for selection. ... (λ) vectors (also cosmids): up to 50 kb BAC (bacterial artificial chromosome): ...

http://www.bios.niu.edu/johns/bioinf_intro/Recombinant%20DNA%20Technology.ppt

Date added: May 8, 2012 - Views: 54

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Chem 360 Lecture slides - Weston

Prokaryotes don’t glycosylate proteins Plasmids ... sizes when insert included Cloning Vectors 1. Plasmids- 5,000 to 400,000 ... Cosmids- artificially ...

http://www.weston.org/schools/ms/biologyweb/heredity/handouts/recombinant_dna_tech_ppt.ppt

Date added: May 2, 2013 - Views: 43

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Slide 1

... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...

http://classes.biology.ucsd.edu/old.web.classes/bimm120.WI06/john_buchanan_lec2.ppt

Date added: September 15, 2012 - Views: 19

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PowerPoint Presentation

Cloning Vectors Target Gene ... structure Construct expression plasmids for functional studies of Protein X in cells Mutagenize ... Bacteriophage-based Cosmids: ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 111

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Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more commonly ... recombinant plasmids containing inserted fragments up ...

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2551.ppt

Date added: October 7, 2011 - Views: 292

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Recombinant DNA Technology for the non-science major.

Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.

http://cogs.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: October 16, 2012 - Views: 30

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 76

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Slide 1

Linkage and Mapping

http://web.as.uky.edu/biology/faculty/harrison/bio621/S12/mappingii.ppt

Date added: August 2, 2013 - Views: 1

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Enzyme Mechanisms - Illinois Institute of Technology

... Shuttle vectors These are plasmids that can operate in ... inserts up to 40 kbp in size Cosmids in action (fig. 12.9) Shuttle vectors These are plasmids that ...

http://csrri.iit.edu/~howard/biochem/lecppt/mobio_methods.ppt

Date added: May 12, 2013 - Views: 5

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Slide 1

Cosmids have an origin, selectable marker, ... Size of the insert DNA is limited in plasmid cloning vectors, and plasmids carrying more than 5–10 kb are often unstable.

http://www.auburn.edu/academic/classes/biol/3020/lecture_ppts/L14_DNA%20Cloning_14F.ppt

Date added: November 5, 2014 - Views: 2

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Inquiry into Life Twelfth Edition - Regis University ...

vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 27

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids phasmids Phagemids BAC ... Yeast episomal plasmids YEps were ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_4.ppt

Date added: June 18, 2014 - Views: 6

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Exploring Genes - Mount Saint Mary College

Exploring Genes Recombinant Technololgy ...

http://faculty.msmc.edu/turkenko/html/exploring_genes.ppt

Date added: May 17, 2013 - Views: 22

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PowerPoint Presentation

Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 20

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Slajd 1 - Strona Główna - Politechnika Gdańska

Essential Features of Plasmids Most vectors currently in use carry a replicon from pMB1 or Col ... Cosmids M13 Phage Vectors Copyright © The McGraw-Hill Companies ...

http://www.pg.gda.pl/chem/Katedry/Mikrobiologia/W2-IG2.ppt

Date added: November 21, 2011 - Views: 29

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PowerPoint Presentation

... specialized plasmids Vectors containing bacteriophage RNA ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 99

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Restriction enzymes - Home | Department of Biology ...

Vectors. Plasmid – 10-20kbp ... Cosmids – a plasmid + Lambda DNA hybrid, 50 kb inserts. Bacterial ... F plasmids that can accept 150-300 kb inserts. Yeast ...

http://biology.ufl.edu/COURSES/PCB3063/2010spring/miyamoto/notes/biotechnology-Fan.pptx

Date added: February 5, 2013 - Views: 2