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Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...
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Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...
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cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 225
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
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3 Basic Principles of Recombinant DNA Technology. ... Cosmids. B. Vectors for Other Organisms. ... Engineered Ti plasmids lack the tumor-causing genes, ...
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Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.
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... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors ... vectors Phage Insertion Cosmids ...
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PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
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Plasmids and other types of cloning vectors were used by Human Genome Project researchers to copy ... Cosmids are artificially constructed cloning vectors that ...
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Cloning Vectors Recombinant DNA ... 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) ...
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Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...
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Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.
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Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase Splice into Cloning Vehicle Plasmids ... Cosmids Other Vectors ...
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H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...
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Vectors (cloning vehicles) Enzymes for cutting and joining the DNA fragments. ... Types of Vectors. Plasmids. Cosmids. Fosmids. Phages. Yeast Artificial Chromosomes ...
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Examples of large-insert cloning vectors are cosmids, ... by cellular DNases.Picture Cosmid features and uses Cosmids are predominantly plasmids with a bacterial ...
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... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression ...
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Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) ... Molecular Cloning Author: Lab
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Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
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Plasmids: independently ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ... Recombinant DNA Technology Needles in Haystacks How to ...
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Lecture 5 Recombinant DNA Technology Cloning Vectors Gene Libraries Clone Identification and Characterization Reading: Chapter 9 Molecular Biology syllabus web site
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Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...
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Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...
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... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids ... are also vectors Like cosmids have aspects of ...
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Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...
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Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...
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... vector Size of library depends on abundance of message Bacteriophage lambda insertion vectors or plasmids are used for ... can be accommodated by cosmids ...
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Cloning Vectors Enable DNA molecules ... Examples: Cosmids PACs BACs Plasmid Vectors ... Allows you to select bacteria that have plasmids with an ...
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Biotechnology and Genetic Engineering PBIO 4500 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids ...
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... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, ... color change Plasmids vectors Easy to isolate and ...
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... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.
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... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:
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Plasmids for cloning carry drug resistance genes that are used for selection. ... (λ) vectors (also cosmids): up to 50 kb BAC (bacterial artificial chromosome): ...
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Prokaryotes don’t glycosylate proteins Plasmids ... sizes when insert included Cloning Vectors 1. Plasmids- 5,000 to 400,000 ... Cosmids- artificially ...
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... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...
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Cloning Vectors Target Gene ... structure Construct expression plasmids for functional studies of Protein X in cells Mutagenize ... Bacteriophage-based Cosmids: ...
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Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more commonly ... recombinant plasmids containing inserted fragments up ...
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Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.
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Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...
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Linkage and Mapping
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... Shuttle vectors These are plasmids that can operate in ... inserts up to 40 kbp in size Cosmids in action (fig. 12.9) Shuttle vectors These are plasmids that ...
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Cosmids have an origin, selectable marker, ... Size of the insert DNA is limited in plasmid cloning vectors, and plasmids carrying more than 5–10 kb are often unstable.
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vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...
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... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids phasmids Phagemids BAC ... Yeast episomal plasmids YEps were ...
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Exploring Genes Recombinant Technololgy ...
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Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...
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Essential Features of Plasmids Most vectors currently in use carry a replicon from pMB1 or Col ... Cosmids M13 Phage Vectors Copyright © The McGraw-Hill Companies ...
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... specialized plasmids Vectors containing bacteriophage RNA ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a ...
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Vectors. Plasmid – 10-20kbp ... Cosmids – a plasmid + Lambda DNA hybrid, 50 kb inserts. Bacterial ... F plasmids that can accept 150-300 kb inserts. Yeast ...
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