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Cloning into Plasmids - Buffalo State College...

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Cloning into Plasmids - Buffalo State College...

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 94

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... Cosmids. B. Vectors for Other Organisms. ... Engineered Ti plasmids lack the tumor-causing genes, ...

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 164

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Cloning - Sam Houston State University

Plasmids and other types of cloning vectors were used by Human Genome Project researchers to copy ... Cosmids are artificially constructed cloning vectors that ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 26

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Trends in Biotechnology

Trends in Biotechnology 110509 3b – Vectors Chapter 3b - outline V. Cloning Vectors A. Bacterial Vectors 1. Plasmids 2. Bacteriophage 3.

http://web.hallym.ac.kr/~colinbrowne/TrendsBiotechnology_files/110509%203b.ppt

Date added: December 5, 2013 - Views: 10

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 3

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Slide 1

Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 1

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No Slide Title

... “sticky” or blunt ends after cleavage Ligation and vectors - plasmids, phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, ...

http://web.bio.utk.edu/humangenetics/lecture/molec_tools06sep02.ppt

Date added: November 28, 2013 - Views: 4

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 27

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 4

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Inquiry into Life Twelfth Edition - McGraw-Hill...

... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 32

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PowerPoint Presentation

Lambda Bacteriophage Vectors cos cos cos Selection: Only recombinant phage produce plaques. 1. Size ... Cosmids: Plasmids that contain l cos sites.

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: January 4, 2015 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

... but it can also be 125I Animations for two related uses of expression vectors Expression ... cloning vectors including plasmids, ... cDNA library construction ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 37

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Introduction to molecular cloning - ABE

Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmidsplasmids containing DNA sequences (cos) ... Molecular Cloning Author: Lab

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 86

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PowerPoint Presentation

Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 21

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 76

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Inquiry into Life Twelfth Edition - Regis...

vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 30

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PowerPoint Presentation

Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 14

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Enzyme Mechanisms - Illinois Institute of...

... Shuttle vectors These are plasmids that can operate in ... inserts up to 40 kbp in size Cosmids in action (fig. 12.9) Shuttle vectors These are plasmids that ...

http://csrri.iit.edu/~howard/biochem/lecppt/mobio_methods.ppt

Date added: May 12, 2013 - Views: 5

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Viral Vectors - University of Saskatchewan

Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: February 19, 2012 - Views: 39

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cDNA Library - MB210 Microbial Biotechnology - MB

cDNA Library MB206 Angelia 09 * Ligation to vector Any vectors with an EcoRI site would suitable for cloning the cDNA. The process : Dephosphorylate the vector with ...

http://mb206.wikispaces.com/file/view/MB206_fhs_Int_011_AT_Jan09.ppt

Date added: December 29, 2012 - Views: 95

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Slide 1

Vectors. Plasmid – 10-20kbp ... Cosmids – a plasmid + Lambda DNA hybrid, 50 kb inserts. Bacterial ... F plasmids that can accept 150-300 kb inserts. Yeast ...

http://biology.ufl.edu/courses/pcb3063/2011spring/miyamoto/notes/Biotechnology%20Fan.pptx

Date added: April 20, 2015 - Views: 1

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PowerPoint Presentation

BIOL 2416 Genetics Chapter 8: Mapping and Sequencing of Genomes

http://learning.hccs.edu/faculty/jessica.moody/biol2416/power-point-presentations/chapter-8

Date added: August 8, 2013 - Views: 5

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Foundations in Microbiology

Chapter 10 Genetic Engineering: A Revolution in Molecular Biology

http://learning.hccs.edu/faculty/harold.njemanze/micro2420-1/micro-powerpoint/chapter-10cr/at_download/file

Date added: December 24, 2014 - Views: 2

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الشريحة 1 - Majmaah University

Plasmids. Bacteriophages. Cosmids: ... Molecular cloning vectors. Gene cloning. Insulin production by gene cloning. BEST WISHES. Author: Emad A. Morad Created Date:

http://faculty.mu.edu.sa/public/uploads/1385287329.674610.%20gene%20cloning.pptx

Date added: December 16, 2014 - Views: 2

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PowerPoint Presentation

Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function

http://public.gettysburg.edu/~hiraizum/bio211/0909.ppt

Date added: September 8, 2014 - Views: 1

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基因重組原理與技術

... (Cloning Vectors) 細菌 – 質體 (plasmids) 噬菌體 (bacteriophages) 黏接質體 ... (Cloning Vectors) 噬菌體與 cosmids 舉例 轉殖載體 ...

http://elearning.ice.ntnu.edu.tw/km/Data/Teacher/31972/data/%E6%88%91%E7%9A%84%E5%80%8B%E4%BA%BA%E6%96%87%E4%BB%B6/lecture%202%20-%20%E5%9F%BA%E5%9B%A0%E9%87%8D%E7%B5%84%E5%8E%9F%E7%90%86%E8%88%87%E6%8A%80%E8%A1%93-eef23.ppt

Date added: March 29, 2012 - Views: 2

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Gene cloning tutorial - University of Leicester

Cosmids can be used to ... These vectors are then ... Once you have transformed your cells with your ligation mix you must select for recombinant plasmids ...

http://www2.le.ac.uk/departments/genetics/vgec/downloads/educators-over-18s/Gene%20Cloning/2%20CloningPowerPoint.pptx

Date added: June 10, 2013 - Views: 41

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Nitrogen Base - SVPM

Vectors : The extrachromosomal DNA that carries desired gene to the host cell is called gene cloning vector. Eg. Plasmids, viral DNA, Cosmids etc. Plamids : ...

http://pharmacy.svpm.org.in/ppt/Genetic%20Engineering.pptx

Date added: September 21, 2014 - Views: 1

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PowerPoint 演示文稿

... Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors ... libraries from prokaryotes in plasmids where the insert size is 5-10kb ...

http://202.114.65.51/fzjx/fzswx/wlkt/ppt2005/application1.ppt

Date added: March 2, 2012 - Views: 9

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Chpter 4 - 建構中

Chapter 4 Molecular Cloning Methods Gene Cloning The Role of Restriction Endonuclease Vectors Plasmids : carriers ... Cosmids * cohesive ends of l phage DNA ...

http://memo.cgu.edu.tw/jau-song/slide/G/Molecular%20%20Biology%20%20Chpter%204.ppt

Date added: October 29, 2012 - Views: 21

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PowerPoint 프레젠테이션

Common vectors used in libraries Plasmids Up to 10kbp inserts Modified Lambda phage Up to 20kbp inserts/40kbp for cosmids Artificial cloning vectors BAC- Vectors ...

http://ani.gntech.ac.kr/html/data/genetics%20engneering-ch5.ppt

Date added: February 8, 2014 - Views: 13

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Plasmids and Vectors - Molecular Biology for...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: January 31, 2015 - Views: 2

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Section D - Prokaryotic and Eukaryotic Chromosome...

Section G – Gene manipulation Contents G1 DNA cloning: an overview DNA cloning, Hosts and vectors, Subcloning, DNA libraries, Screening libraries, Analysis of a ...

http://fzswx.gxu.edu.cn/course/Section%20G%20-%20Gene%20manipulation.ppt

Date added: November 27, 2013 - Views: 11

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Vectors

Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: September 11, 2011 - Views: 265

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Computational Aspects of Biological Sequences

Basic Molecular Biology Many slides by Omkar Deshpande

http://www.cs.toronto.edu/~brudno/csc2417_10/2417intro.ppt

Date added: April 8, 2015 - Views: 1

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ai.stanford.edu

ai.stanford.edu

http://ai.stanford.edu/~serafim/CS262_2009/presentations/lecture9.pptx

Date added: February 8, 2015 - Views: 3

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Chpter 4

Chapter 4 Molecular Cloning Methods

http://memo.cgu.edu.tw/tzu-chien/MB2009/Yu2009-1.ppt

Date added: May 10, 2013 - Views: 20

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第六章 - 同济大学医学院

Vectors: plasmid, λphage, ... Plasmids usually occur naturally in bacteria, ... Cosmids can be used to build genomic library.

http://med.tongji.edu.cn/yzkc/DT_UploadFile/20101224183754546.ppt

Date added: June 13, 2012 - Views: 21

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Lambda Vectors and their replication - Department...

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 39

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 226

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DNA cloning intro.ppt - Biology

PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 34

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PowerPoint Presentation

Gene expression * Expression vectors: ... E. coli cloning vector (circular): plasmids (质粒 ... plasmid-bacteriophage l hybrids (cosmids) (考斯质粒 ...

http://202.114.65.51/fzjx/fzswx/wlkt/ppt2008/2008chapter21.ppt

Date added: November 11, 2011 - Views: 13

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 20

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Restriction Enzymes, Vectors, and Genetic...

... Linear DNA Can insert up to 15 kb fragments Phage Insertion Cosmids Don’t ... Eco R1 Vectors A self-replicating DNA ... of Vectors Plasmids ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 33

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Chapter 4 Molecular Cloning Methods - Xiamen...

Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 172

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Section D - Prokaryotic and Eukaryotic Chromosome...

H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 14

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Slide 1

... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...

http://classes.biology.ucsd.edu/old.web.classes/bimm120.WI06/john_buchanan_lec2.ppt

Date added: September 15, 2012 - Views: 20

ppt
Slide 1

... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, ... color change Plasmids vectors Easy to isolate and ...

http://classes.biology.ucsd.edu/bimm120.WI07/genetics2_doran_fa06.ppt

Date added: September 7, 2012 - Views: 31

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PowerPoint Presentation

Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures/chapter_08.ppt

Date added: April 10, 2015 - Views: 1