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Plasmids and Vectors - Molecular Biology for Secondary ...

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Plasmids and Vectors - Molecular Biology for Secondary ...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

http://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: October 16, 2011 - Views: 199

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Vectors - College of Computer, Mathematical, and Natural Sciences

Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Date added: July 18, 2013 - Views: 44

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 223

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Cloning into Plasmids - Buffalo State College Faculty and ...

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 93

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Lambda Vectors and their replication - Department Of ...

Lambda vectors and their ... Plasmids- small circular DNA molecules which can replicate their DNA independently ... Cosmids- are hybrid vectors of l phage and ...

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 35

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Restriction Enzymes, Vectors, and Genetic Libraries

... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors ... vectors Phage Insertion Cosmids ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 27

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Chapter 4 Molecular Cloning Methods

Chapter 4 Molecular Cloning Methods Introduction The significance of gene cloning To elucidate the structure and function of genes. i.e.: investigating hGH gene hGH ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 165

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Section D - Prokaryotic and Eukaryotic Chromosome Structure

H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes ... Hybrid plasmid-M13 vectors H3 Cosmids, ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 11

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3 Basic Principles of Recombinant DNA Technology - User Homepages

3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. ... Cosmids. B. Vectors for Other Organisms. 1. Yeast Artificial Chromosomes ...

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 150

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Chapter 3 Methods in Molecular Biology and Genetic Engineering

Vectors contain selectable markers ... vectors may be bacterial plasmids, phages, cosmids, ... 3 Methods in Molecular Biology and Genetic Engineering 3.1 ...

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202013%20Biol203/Lecture4-Chap3_Sept17.ppt

Date added: October 23, 2013 - Views: 10

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 20

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Genetic Engineering

Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase Splice into Cloning Vehicle Plasmids ... Cosmids Other Vectors ...

p://people.uncw.edu/sizemorer

Date added: November 5, 2014 - Views: 6

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Gene Cloning

Cloning Vectors Recombinant DNA ... 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) ...

http://xa.yimg.com/kq/groups/24240295/1734630456/name/lecture+04.ppt

Date added: February 18, 2014 - Views: 12

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 3

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Vectors for Even Larger Genomic DNA Inserts

General Microbiology (Micr300) Lecture 11 Biotechnology (Text Chapters: 10.15-10.17; 31.1-31.10)

http://instructional1.calstatela.edu/hxu3/Micr300/S06micr300Lec11final.ppt

Date added: May 29, 2013 - Views: 26

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Setting up a transformation--how will the competent cells be ...

Setting up a transformation--how will the competent ... F plasmid-based Cosmids: 5 kb plasmids, ... vectors for propagation in E. coli Plasmids Bacteriophage ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: November 15, 2011 - Views: 71

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Cloning - Sam Houston State University - Texas - Carnegie ...

Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 19

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules ... Examples: Cosmids PACs BACs Plasmid Vectors ... Allows you to select bacteria that have plasmids with an ...

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 25

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_3.ppt

Date added: July 1, 2014 - Views: 4

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PowerPoint Presentation

Examples of large-insert cloning vectors are cosmids, ... by cellular DNases.Picture Cosmid features and uses Cosmids are predominantly plasmids with a bacterial ...

http://forest.mtu.edu/classes/fw3300/BL3300%202011/2011%20%20Chapt%2003%20091904%20cpj.ppt

Date added: May 21, 2012 - Views: 55

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Molecular Cloning - Advances in Bioscience Education (ABE)

Foreign DNA Host organism Vector DNA for cloning Means of inserting ... Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmidsplasmids ...

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 83

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PowerPoint Presentation

Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures-2013/chapter_08.ppt

Date added: October 2, 2014 - Views: 3

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Recombinant DNA Technology - NIU Department of Biological ...

Recombinant DNA Technology ... Use these phage to infect new E. coli. Cosmids are similar to phage vectors: ... The recombinant plasmids are transformed into E. coli ...

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 254

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Inquiry into Life Twelfth Edition

... the site where DNA replication begins There are 2 major classes of vectors: Plasmids Phages Plasmids ... are also vectors Like cosmids have aspects of ...

http://highered.mheducation.com/sites/dl/free/0073525324/891545/Chapter_04_lecture.ppt

Date added: July 10, 2014 - Views: 2

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids . Title ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 26

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 3

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Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more ... Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2551.ppt

Date added: October 7, 2011 - Views: 281

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PowerPoint Presentation

Central Dogma of Biology DNA RNA Protein

http://www.molecularbiology.yolasite.com/resources/Lecture%202.ppt

Date added: October 13, 2014 - Views: 3

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Biotechnology and Genetic Engineering-PBIO 450/550

Library screening Eukaryotic ... How to make and screen gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 35

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Recombinant DNA Technology - NIU Department of Biological ...

Plasmids for cloning carry drug resistance genes that are used for selection. ... (λ) vectors (also cosmids): up to 50 kb BAC (bacterial artificial chromosome): ...

http://www.bios.niu.edu/johns/bioinf_intro/Recombinant%20DNA%20Technology.ppt

Date added: May 8, 2012 - Views: 54

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Inquiry into Life Twelfth Edition - McGraw-Hill Education

... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 26

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Inquiry into Life Twelfth Edition - Regis University ...

vectors: Plasmids; Phages . Selectable marker, Restriction sites for cloning. ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. Contain ...

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 25

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PowerPoint Presentation

Ligase: joins (seals) the ends of DNA Vectors: DNA molecule that can replicate & is used to transport foreign DNA Four types of Vectors: Plasmids; Bacteriophage ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 14

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Part II: Recombinant DNA Technology . ppt

Recombinant DNA Technology

http://rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: May 2, 2013 - Views: 40

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Chem 360 Lecture slides

Recombinant DNA Technology

http://www.weston.org/schools/ms/biologyweb/heredity/handouts/recombinant_dna_tech_ppt.ppt

Date added: May 2, 2013 - Views: 42

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PowerPoint Presentation

Cloning Vectors Target Gene ... structure Construct expression plasmids for functional studies of Protein X in cells Mutagenize ... Bacteriophage-based Cosmids: ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 109

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Slide 1

... genetic element used to propagate and express genes of interest in bacteria Plasmids, phages, cosmids, ... color change Plasmids vectors Easy to isolate and ...

http://classes.biology.ucsd.edu/bimm120.WI07/genetics2_doran_fa06.ppt

Date added: September 7, 2012 - Views: 29

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Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids phasmids Phagemids BAC ... Yeast episomal plasmids YEps were ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_4.ppt

Date added: June 18, 2014 - Views: 6

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Slide 1

... when foreign DNA is inserted Recombinant phage DNA can be packaged into viral capsids and used to infect a host cell Cosmids - plasmids ... Plasmids vectors ...

http://classes.biology.ucsd.edu/old.web.classes/bimm120.WI06/john_buchanan_lec2.ppt

Date added: September 15, 2012 - Views: 19

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Slide 1

Lecture 14 – Gene Cloning Based on Chapter 08 - Genomics: The Mapping and Sequencing of Genomes Copyright © 2010 Pearson Education Inc.

http://www.auburn.edu/academic/classes/biol/3020/lecture_ppts/L14_DNA%20Cloning_14F.ppt

Date added: November 5, 2014 - Views: 2

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Exploring Genes - Login - My.msmc.edu

Exploring Genes Recombinant ... or cohesive ends Cloning Vectors Plasmids and ... Recombinant DNA What are cosmids? combinations of plasmids and lambda phages can ...

http://faculty.msmc.edu/turkenko/html/exploring_genes.ppt

Date added: May 17, 2013 - Views: 21

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 74

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Enzyme Mechanisms

Recombinant DNA Andy Howard Introductory Biochemistry 18 November 2008

http://csrri.iit.edu/~howard/biochem/lecppt/mobio_methods.ppt

Date added: May 12, 2013 - Views: 5

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Restriction enzymes

Vectors. Plasmid – 10-20kbp ... Cosmids – a plasmid + Lambda DNA hybrid, 50 kb inserts. Bacterial ... F plasmids that can accept 150-300 kb inserts. Yeast ...

http://biology.ufl.edu/COURSES/PCB3063/2010spring/miyamoto/notes/biotechnology-Fan.pptx

Date added: February 5, 2013 - Views: 2

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PowerPoint Presentation

... VECTOR Vectors carry the target gene Cloning Vectors segments of DNA that carry target gene; allow reproduction of gene plasmids phages cosmids part ...

http://people.uncw.edu/doddd/Biology%20335/PowerPoints/DNAtech.ppt

Date added: March 10, 2012 - Views: 26

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Recombinant DNA Technology for the non-science major.

Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.

http://cogs.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: October 16, 2012 - Views: 28

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PowerPoint Presentation

Mobilizing DNA: vectors for propagation in E. coli Plasmids ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 97

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Viral Vectors - University of Saskatchewan

Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: February 19, 2012 - Views: 39

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Slide 1

The original plate is used to pick bacterial cells with recombinant plasmids that hybridized ... Plasmids Phages Cosmids New generation vectors: Bacterial ...

http://higheredbcs.wiley.com/legacy/college/allison/1118059816/ppt/ch08.ppt

Date added: August 2, 2013 - Views: 23