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Plasmid Cloning Vectors Plasmids are circular, double-stranded DNA molecules that exist in bacteria and in the nuclei of some eukaryotic cells.
Date added: July 18, 2013 - Views: 44
Plasmids - are tiny circular pieces of DNA that are commonly found in bacteria. Why Plasmids are Good Cloning Vectors small size (easy to manipulate and isolate) ...
Date added: January 23, 2013 - Views: 34
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 93
Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...
Date added: October 16, 2011 - Views: 201
Promega Corporation Education Resources Unit 006 To determine whether your bacterial host cells have “taken up” your plasmid when you are cloning, most plasmids ...
Date added: September 19, 2012 - Views: 77
Recombinant DNA. Cloning & Transformation. What do you know about cloning? ... Vectors are often Plasmids. What is a plasmid? Circular, autonomous molecules of DNA.
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Inserting genes into Plasmids. The real-life application of what we have just learnt, occurs when geneticists insert an animal or plant gene into plasmids.
Date added: June 16, 2012 - Views: 31
Gene Cloning Techniques for gene cloning enable scientists to prepare multiple identical copies of gene-sized pieces of DNA. Most methods for cloning pieces of DNA ...
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Plasmids and other cloning vectors 7.1 DNA cloning with plasmid vectors Recombinant DNA technology depends on the ability to produce large numbers of identical DNA ...
Date added: January 27, 2012 - Views: 25
Cloning Vectors. A. The DNA of interest is inserted into a cloning vector to transport it into the host cell. The vector needs to have the following characteristics:
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Recombinant DNA Cont. Plasmids can carry up to 20,000 bp of foreign DNA. Besides bacterial plasmids, some other cloning vectors include viruses, bacteria artificial ...
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Recombinant DNA cloning technology Chapter 7 DNA cloning To obtain large amounts of pure DNA Procedure Isolate DNA Use restriction enzymes to cut DNA Ligate fragments ...
Date added: October 8, 2011 - Views: 107
Biotechnology and Recombinant DNA ... outside the cell Plasmids are common vectors Often contain ... Restriction Enzymes Vectors Cloning plasmid Kary ...
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Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...
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Cloning vectors are DNA molecules that are used to "transport ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from ...
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Recombinant DNA and Cloning Riyanda N G (10198) Vina E A (10221) Arini N (10268) Suluh N (10302) Cloning Definition The word "clone" has several different meanings in ...
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Title: Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis Author: Montarop Yamabhai Created Date: 7/18/2004 7:38:52 AM
Date added: October 7, 2011 - Views: 290
Plasmids useful as cloning vectors must have: An origin of replication. A selectable marker (antibiotic resistance gene, such as ampr and tetr).
Date added: October 4, 2011 - Views: 156
Lecture # 04 Cloning Vectors Recombinant DNA Technology Common General Cloning Strategy Target DNA from donor organism extracted, cut with compatible restriction ...
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Outline 13.1 Cloning 13.2 DNA Libraries 13 ... DNA fragments to the sticky ends of plasmid Cloning Vectors Plasmids that can be modified to carry new genes ...
Date added: March 3, 2012 - Views: 41
Title: Recombinant DNA and Genetic Engineering Author: Christine Evers Last modified by: Information Technology Created Date: 10/15/2001 10:36:33 PM
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Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.
Date added: September 30, 2013 - Views: 36
Genetic Engineering = inserting a foreign gene of ... plasmid = Recombinant plasmid Plasmids can be used as cloning vectors to get the recombinant plasmid ...
Date added: April 1, 2013 - Views: 41
Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.
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Cloning:Recombinant DNA ... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors need…
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Restriction Endonucleases, Plasmids, and Transformation Restriction Endonucleases Enzymes purified from bacteria Protect bacterial cell from viruses by degrading ...
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This enables the vectors to readily ligate DNA sequences with compatible ends ... Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis
Date added: September 27, 2011 - Views: 451
Outline the procedures for producing plasmid and phage vectors. Isolation of vector and gene-source DNA - bacterial plasmids ... Cloning vector a DNA ...
Date added: May 2, 2013 - Views: 38
Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...
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Recombinant DNA Chapter 18 Learning Objectives Define Clone and DNA Cloning List the three steps of production of recombinant DNA Describe the characteristics and ...
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Multiple Cloning Sites. Many cloning vectors contain a multiple cloning site or polylinker: a DNA segment with several unique sites for restriction endo- nucleases ...
Date added: August 6, 2013 - Views: 3
Chapter 9 – DNA-Based Information Technologies Recombinant DNA molecules are constructed with DNA from different sources Recombinant DNA molecules are created often ...
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Development of Plasmid Vectors Plasmids serve as important tools in genetics and ... In Molecular Cloning One of the major uses of plasmids is to make large amounts ...
Date added: October 25, 2011 - Views: 55
Microbial Genomics Genetic engineering ... Outline of lecture Traditional recombinant DNA methodology including; restriction enzymes, plasmids as cloning vectors, ...
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Exploring Genes Recombinant ... What advantages do these modified viruses have over plasmids? Cloning Vectors What are some of the advantages of using M13 phage as a ...
Date added: May 17, 2013 - Views: 22
H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes in Saccharomyces cerevisiae. Based on 2 micron (2m ...
Date added: February 13, 2012 - Views: 12
Vectors for cloning 5. Using plasmids as vectors Isolating the gene from the gene source DNA is divided into genes (units of inheritance) ...
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Recombinant Plasmids ...
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DNA Cloning and Characterization ... Cloning vectors are plasmids that have been genetically engineered to reduce unneeded DNA and to introduce selectable markers ...
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Most cloning vectors use a system for detecting the presence of a recombinant insert, ... The recombinant plasmids are transformed into E. coli using heat plus ...
Date added: May 8, 2012 - Views: 54
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 224
Cloning Vectors Target Gene Selection and Acquisition Restriction Endonucleases Polymerase Chain Reaction (PCR) ... Plasmids used for genomic and cDNA cloning.
Date added: October 7, 2011 - Views: 111
Cloning and Sequencing Project overview Background Project will have you cloning the gene that codes for the enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
Date added: May 3, 2013 - Views: 34
Recombinant DNA Technology ... Plasmids for cloning carry drug resistance genes that are used for selection. ... Most cloning vectors have a multiple cloning site, ...
Date added: October 7, 2011 - Views: 256
Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) from bacteriophage λ used to clone larger ...
Date added: November 5, 2011 - Views: 83