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Vectors - College of Computer, Mathematical, and Natural Sciences

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Vectors - College of Computer, Mathematical, and Natural Sciences

Plasmid Cloning Vectors Plasmids are circular, double-stranded DNA molecules that exist in bacteria and in the nuclei of some eukaryotic cells.

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: September 11, 2011 - Views: 262

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Cloning into Plasmids - Buffalo State College Faculty and ...

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 93

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Plasmids and Vectors - Molecular Biology for Secondary ...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

http://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: October 16, 2011 - Views: 198

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Basics of Molecular Cloning - www.promega.com

Promega Corporation Education Resources Unit 006 To determine whether your bacterial host cells have “taken up” your plasmid when you are cloning, most plasmids ...

http://www.promega.com/~/media/files/resources/education%20and%20training/unit%206/basics%20of%20molecular%20cloning.ppt?la=en

Date added: September 19, 2012 - Views: 76

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DNA cloning intro.ppt - Biology

Plasmids - are tiny circular pieces of DNA that are commonly found in bacteria. Why Plasmids are Good Cloning Vectors small size (easy to manipulate and isolate) ...

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 34

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Gene Cloning - Wikispaces - SandersBiologyStuff - home

Gene Cloning Techniques for gene cloning enable scientists to prepare multiple identical copies of gene-sized pieces of DNA. Most methods for cloning pieces of DNA ...

http://sandersbiologystuff.wikispaces.com/file/view/Gene+Cloning.ppt

Date added: December 12, 2013 - Views: 13

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Recombinant DNA - Egg Harbor Township Schools

Recombinant DNA. Cloning & Transformation. What do you know about cloning? ... Vectors are often Plasmids. What is a plasmid? Circular, autonomous molecules of DNA.

http://www.eht.k12.nj.us/~oneilj/NEURO_GENETICS_files/Genetics/Recombinant%20DNA_cloning_plasmids_vectors.pptx

Date added: April 17, 2014 - Views: 6

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16.2 In vivo gene cloning – the use of vectors

Inserting genes into Plasmids. The real-life application of what we have just learnt, occurs when geneticists insert an animal or plant gene into plasmids.

http://vbio.weebly.com/uploads/2/8/2/4/2824362/16.2_-_in_vivo_gene_cloning_-_the_use_of_vectors.pptx

Date added: June 16, 2012 - Views: 31

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Cloning - Sam Houston State University - Texas - Carnegie ...

Recombinant DNA Cont. Plasmids can carry up to 20,000 bp of foreign DNA. Besides bacterial plasmids, some other cloning vectors include viruses, bacteria artificial ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 19

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Recombinant DNA cloning technology - Bilkent University ...

Recombinant DNA cloning technology Chapter 7 DNA cloning To obtain large amounts of pure DNA Procedure Isolate DNA Use restriction enzymes to cut DNA Ligate fragments ...

http://www.fen.bilkent.edu.tr/~konu/Recombinant_DNA.ppt

Date added: October 8, 2011 - Views: 105

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 3

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Slide 1

Development of Plasmid Vectors Plasmids serve as important tools in genetics and ... In Molecular Cloning One of the major uses of plasmids is to make large amounts ...

http://abe.leeward.hawaii.edu/WebSharing/Lecture,%20%20Plasmid%20isolation.ppt

Date added: October 25, 2011 - Views: 54

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3 Basic Principles of Recombinant DNA Technology - User Homepages

Cloning Vectors. A. The DNA of interest is inserted into a cloning vector to transport it into the host cell. The vector needs to have the following characteristics:

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 150

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No Slide Title

Molecular Genetics Techniques BIT 220 Chapter 20 What is Cloning? Recombinant DNA technologies Restriction Endonucleases Why use REs? Plasmids Cloning Vectors Plasmid ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 74

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Recombinant DNA - Raritan Valley Community College

Recombinant DNA Chapter 18 Learning Objectives Define Clone and DNA Cloning List the three steps of production of recombinant DNA Describe the characteristics and ...

http://www.raritanval.edu/uploadedFiles/faculty/sci/part-time/Cleverley/Recombinant%20DNA%20extended.ppt

Date added: August 15, 2013 - Views: 18

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Gene Cloning

Lecture # 04 Cloning Vectors Recombinant DNA Technology Common General Cloning Strategy Target DNA from donor organism extracted, cut with compatible restriction ...

http://xa.yimg.com/kq/groups/24240295/1734630456/name/lecture+04.ppt

Date added: February 18, 2014 - Views: 12

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Vectors for Even Larger Genomic DNA Inserts

General Microbiology (Micr300) Lecture 11 Biotechnology (Text Chapters: 10.15-10.17; 31.1-31.10)

http://instructional1.calstatela.edu/hxu3/Micr300/S06micr300Lec11final.ppt

Date added: May 29, 2013 - Views: 24

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Lambda Vectors and their replication - Department Of ...

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 33

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Recombinant DNA

Fact Sheet Describing Recombinant DNA and Elements Utilizing Recombinant DNA Such as Plasmids and ... genes Transformation methods Cloning Vectors Plasmid ...

http://www.aryansexport.com/PPT/Recombinant%20DNA%20technology.ppt

Date added: June 26, 2014 - Views: 4

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Chapter 13 Slides - people.Virginia.EDU

Outline 13.1 Cloning 13.2 DNA Libraries 13 ... DNA fragments to the sticky ends of plasmid Cloning Vectors Plasmids that can be modified to carry new genes ...

http://people.virginia.edu/~cmg/slides/chapter_13.ppt

Date added: March 3, 2012 - Views: 41

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Restriction Endonucleases and Plasmids

Restriction Endonucleases, Plasmids, and Transformation Restriction Endonucleases Enzymes purified from bacteria Protect bacterial cell from viruses by degrading ...

http://www.fevanet.com/chemistry/SBI4UO_WEB/restriction%20endonucleases_plasmid_transformation.ppt

Date added: November 8, 2014 - Views: 1

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Biotechnology and Recombinant DNA - Southwestern College : Home

Biotechnology and Recombinant DNA ... outside the cell Plasmids are common vectors Often contain ... Restriction Enzymes Vectors Cloning plasmid Kary ...

http://www2.swccd.edu/~mseteachingresources/msetrshare/biology/brady/B265/Microbiology%20ppt%20new%2007/Biotechnology%20and%20Recombinant%20DNA%20Ch.%209.ppt

Date added: June 22, 2013 - Views: 44

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Recombinant DNA and Genetic Engineering - Salisbury University

Title: Recombinant DNA and Genetic Engineering Author: Christine Evers Last modified by: Information Technology Created Date: 10/15/2001 10:36:33 PM

http://faculty.salisbury.edu/%7Emaholland/101DNA.ppt

Date added: May 11, 2013 - Views: 16

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Recombinant DNA Technology & Cloning

Bacterial Plasmids. Bacterial cells may contain extra-chromosomal DNA called ... Multiple Cloning Sites. Many cloning vectors contain a multiple cloning site or ...

http://lectureug4.files.wordpress.com/2012/03/biotech-lect-2.pptx

Date added: August 6, 2013 - Views: 3

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Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

Title: Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis Author: Montarop Yamabhai Created Date: 7/18/2004 7:38:52 AM

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2551.ppt

Date added: October 7, 2011 - Views: 277

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Recombinant DNA Technology - NIU Department of Biological ...

Recombinant DNA Technology ... Plasmids for cloning carry drug resistance genes that are used for selection. ... Most cloning vectors have a multiple cloning site, ...

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 254

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Chapter 9 – DNA-Based Information Technologies

Chapter 9 – DNA-Based Information Technologies Recombinant DNA molecules are constructed with DNA from different sources Recombinant DNA molecules are created often ...

http://ww2.valdosta.edu/~dgosnell/3601/May%202005/DNA-Based%20Information%20Technologies.ppt

Date added: May 3, 2013 - Views: 17

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Section D - Prokaryotic and Eukaryotic Chromosome Structure

H4 Eukaryotic vectors — Yeast episomal plasmids Vectors for the cloning and expression of genes in Saccharomyces cerevisiae. Based on 2 micron (2m ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 11

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PowerPoint (6.6 MB) - Department of Microbiology

Plasmids useful as cloning vectors must have: An origin of replication. A selectable marker (antibiotic resistance gene, such as ampr and tetr).

http://www.microbio.uab.edu/bedwell/Lectures/BioteachLecture6.24.09.ppt

Date added: October 4, 2011 - Views: 153

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Recombinant DNA and Cloning - Universitas Gadjah Mada

Recombinant DNA and Cloning Riyanda N G (10198) Vina E A (10221) Arini N (10268) Suluh N (10302) Cloning Definition The word "clone" has several different meanings in ...

http://www.faperta.ugm.ac.id/newbie/download/pak_tar/genetika-molekuler/presentasi/Recombinant%20DNA%20and%20Cloning.ppt

Date added: June 20, 2013 - Views: 11

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 20

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Biotechnology and Recombinant DNA

Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.

http://wolfweb.unr.edu/homepage/borgeson/Bio251/10biotechnewa.ppt

Date added: July 28, 2013 - Views: 24

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Chapter 4 Molecular Cloning Methods

Chapter 4 Molecular Cloning Methods Introduction The significance of gene cloning To elucidate the structure and function of genes. i.e.: investigating hGH gene hGH ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 165

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Genetic Engineering - Fremont Union High School District

Genetic Engineering = inserting a foreign gene of ... plasmid = Recombinant plasmid Plasmids can be used as cloning vectors to get the recombinant plasmid ...

http://web.fuhsd.org/pamela_chow/apbio/lectures/Protein%20Synthesis/Genetic%20Engineering.ppt

Date added: April 1, 2013 - Views: 37

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Plasmid - مواقع اعضاء هيئة التدريس | KSU ...

3 Molecular cloning. Plasmids used in genetic engineering are called . vectors. Vector Element . Vector Element .

http://fac.ksu.edu.sa/sites/default/files/plasmid_lab_.pptx

Date added: November 16, 2014 - Views: 1

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Recombinant DNA

Plasmids Serve As Vectors Plasmids are considered "replicons", ... Gene Cloning The second step of the genetic engineering process is gene cloning.

http://biology.uco.edu/personalpages/meeks/Micro%20Take%20Home%20Test%5CRecombinant%20DNA.ppt

Date added: May 10, 2014 - Views: 3

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1. Explain how advances in recombinant DNA technology have ...

Outline the procedures for producing plasmid and phage vectors. Isolation of vector and gene-source DNA - bacterial plasmids ... Cloning vector a DNA ...

http://www.slideboom.com/presentations/download/130116/Ch-16-DNA-Technology-Answers

Date added: May 2, 2013 - Views: 37

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Chapter 13 An Introduction to Cloning and Recombinant DNA

Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...

http://www.bio.brandeis.edu/classes/heredity/Lecture%20Powerpoints/Chapter_13_1.ppt

Date added: August 2, 2013 - Views: 14

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Recombinant Plasmids - Nova Scotia Department of Education

Cloning Genes in Recombinant Plasmids In order for recombinant DNA to be useful it must be made in vast amounts. ... R. plasmids can be useful vectors for genetic ...

http://ssrsbstaff.ednet.ns.ca/emonahan/AP%20Biology/Recombinant%20Plasmids.ppt

Date added: September 27, 2012 - Views: 14

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Inquiry into Life Twelfth Edition - Regis University ...

4-Summary Basic Plasmid Cloning Vectors. pBR322 and the pUC plasmids. pBR322 has . 2 antibiotic resistance genes . Many unique restriction sites for inserting foreign DNA

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 24

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PowerPoint Presentation

DNA-based information technologies Understand basics steps/enzymes/features of DNA cloning Know main types of cloning vectors used - pros & cons of each

http://chemistry.creighton.edu/%7Ejksoukup/DNAtech2_2010.ppt

Date added: February 27, 2014 - Views: 13

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Genetic Engineering

Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase Splice into Cloning Vehicle Plasmids ... Cloning Vector Plasmid Cloning Vectors ...

p://people.uncw.edu/sizemorer

Date added: November 5, 2014 - Views: 6

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Part II: Recombinant DNA Technology . ppt

Recombinant DNA Technology

http://rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: May 2, 2013 - Views: 38

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2.

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 25

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Genetic Engineering and Biotechnology

Vectors for cloning 5. Using plasmids as vectors Isolating the gene from the gene source DNA is divided into genes (units of inheritance) ...

http://www.morganparkcps.org/ourpages/auto/2010/4/15/55162980/Genetic%20Engineering%20and%20Biotechnology.ppt

Date added: October 24, 2013 - Views: 4

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Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

This enables the vectors to readily ligate DNA sequences with compatible ends ... Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2550.ppt

Date added: September 27, 2011 - Views: 444

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Restriction Enzymes, Vectors, and Genetic Libraries

Cloning:Recombinant DNA ... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors need…

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 25

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PowerPoint 簡報 - 建構中

... Western Blotting Compared SYBR Green I Different Cloning Vectors for Different Applications Plasmid-Based Cloning Vectors Naturally occurring plasmids must be ...

http://memo.cgu.edu.tw/kwang-huei/handout/Cellular%20&%20molecular%202007.ppt

Date added: May 15, 2012 - Views: 16

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PowerPoint Presentation

Cloning Vectors Target Gene Selection and Acquisition Restriction Endonucleases Polymerase Chain Reaction (PCR) ... Plasmids used for genomic and cDNA cloning.

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 109