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Vectors - College of Computer, Mathematical, and Natural Sciences

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Vectors - College of Computer, Mathematical, and Natural Sciences

Plasmid Cloning Vectors Plasmids are circular, double-stranded DNA molecules that exist in bacteria and in the nuclei of some eukaryotic cells.

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: September 11, 2011 - Views: 263

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Cloning into Plasmids - Buffalo State College Faculty and ...

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 93

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DNA cloning intro.ppt - Biology

Plasmids - are tiny circular pieces of DNA that are commonly found in bacteria. Why Plasmids are Good Cloning Vectors small size (easy to manipulate and isolate) ...

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 34

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Basics of Molecular Cloning - Promega Corporation

Promega Corporation Education Resources Unit 006 To determine whether your bacterial host cells have “taken up” your plasmid when you are cloning, most plasmids ...

http://www.promega.com/~/media/files/resources/education%20and%20training/unit%206/basics%20of%20molecular%20cloning.ppt?la=en

Date added: September 19, 2012 - Views: 77

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Cloning - Sam Houston State University - Huntsville, TX

Recombinant DNA Cont. Plasmids can carry up to 20,000 bp of foreign DNA. Besides bacterial plasmids, some other cloning vectors include viruses, bacteria artificial ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 21

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Recombinant DNA - Egg Harbor Township Schools

DNA cloning yields multiple copies of a gene of other DNA segment-VOCAB. Derived from bacteria, these products are used to cut strands of DNA at specific locations

http://www.eht.k12.nj.us/~oneilj/NEURO_GENETICS_files/Genetics/Recombinant%20DNA_cloning_plasmids_vectors.pptx

Date added: April 17, 2014 - Views: 7

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No Slide Title

Molecular Genetics Techniques BIT 220 Chapter 20 What is Cloning? Recombinant DNA technologies Restriction Endonucleases Why use REs? Plasmids Cloning Vectors Plasmid ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 76

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Types of Cloning Vectors - Tabuk Portal Universtiy - Tabuk Portal

Types of Cloning Vectors. A. Plasmid Vectors. Plasmids are relatively small, circular DNA molecules. They are commonly used as vectors for DNA fragments up to

http://www.ut.edu.sa/documents/10156/3136643/Lecture+2

Date added: February 12, 2015 - Views: 2

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16.2 In vivo gene cloning – the use of vectors

Inserting genes into Plasmids. ... which of course can be isolated and then introduced into bacterial cells via vectors. ... 16.2 In vivo gene cloning – the use of ...

http://vbio.weebly.com/uploads/2/8/2/4/2824362/16.2_-_in_vivo_gene_cloning_-_the_use_of_vectors.pptx

Date added: June 16, 2012 - Views: 31

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What is Gene Technology

Plasmids and Vectors Aims: Must be able to outline what plasmids and vectors are. Should be able to explain how plasmids and vectors are utilised in research.

http://manorlakesscience.wikispaces.com/file/view/Biology%204.13%20Plasmids%20and%20Vectors.ppt/515311918/Biology%204.13%20Plasmids%20and%20Vectors.ppt

Date added: March 26, 2015 - Views: 1

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Gene Cloning - SandersBiologyStuff - home

Gene Cloning Techniques for gene cloning enable scientists to prepare multiple identical copies of gene-sized pieces of DNA. Most methods for cloning pieces of DNA ...

http://sandersbiologystuff.wikispaces.com/file/view/Gene+Cloning.ppt

Date added: December 12, 2013 - Views: 13

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3 Basic Principles of Recombinant DNA Technology - User Homepages

3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. ... Plant Cloning Vectors. 4. Mammalian Cell Vectors.

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 160

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Cloning - University of Lethbridge

Cloning Using Plasmid Vectors Vector = a molecule used as a vehicle to carry foreign DNA into a host cell Simplest vector = plasmid Features of Plasmids Size ...

http://classes.uleth.ca/200803/biol4200a/Cloning%201%20Lecture.ppt

Date added: September 8, 2014 - Views: 1

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Chapter 4

Plasmids and other cloning vectors 7.1 DNA cloning with plasmid vectors Recombinant DNA technology depends on the ability to produce large numbers of identical DNA ...

http://a32.lehman.cuny.edu/webwurtzel/course/Graduate%20lectures/lecture%205/lecture%205_Recombinant%20DNA%20Techniques.pps

Date added: January 27, 2012 - Views: 25

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Recombinant DNA cloning technology - Faculty of Science at ...

Recombinant DNA cloning technology Chapter 7 DNA cloning To obtain large amounts of pure DNA Procedure Isolate DNA Use restriction enzymes to cut DNA Ligate fragments ...

http://www.fen.bilkent.edu.tr/~konu/Recombinant_DNA.ppt

Date added: October 8, 2011 - Views: 108

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 3

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Part II: Recombinant DNA Technology.ppt - Dr.J.Rajesh Banu Ph ...

... vector Size of library depends on abundance of message Bacteriophage lambda insertion vectors or plasmids are used for cloning The choice depends upon: ...

http://rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: May 2, 2013 - Views: 53

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Recombinant DNA and Cloning - Gadjah Mada University

Recombinant DNA and Cloning Riyanda N G (10198) Vina E A (10221) Arini N (10268) Suluh N (10302) Cloning Definition The word "clone" has several different meanings in ...

http://www.faperta.ugm.ac.id/newbie/download/pak_tar/genetika-molekuler/presentasi/Recombinant%20DNA%20and%20Cloning.ppt

Date added: June 20, 2013 - Views: 13

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Plasmid DNA isolation

Cloning vectors are DNA molecules that are used to "transport ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 1

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PowerPoint Presentation

Cloning vectors are plasmids that have been genetically engineered to reduce unneeded DNA and to introduce selectable markers such as antibiotic resistance genes ...

http://www.uwyo.edu/molecbio/courses/molb-3000/files/13/13-miller-chap-5a-lecture.ppt

Date added: August 2, 2013 - Views: 11

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Recombinant DNA and Genetic Engineering - Salisbury

... Engineering Discovery of Restriction Enzymes Specific Cuts Making Recombinant DNA Making Recombinant DNA Using Plasmids Cloning Vectors Slide 10 Gene ...

http://faculty.salisbury.edu/%7Emaholland/101DNA.ppt

Date added: May 11, 2013 - Views: 16

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Recombinant DNA - Raritan Valley Community College

Recombinant DNA Chapter 18 Learning Objectives Define Clone and DNA Cloning List the three steps of production of recombinant DNA Describe the characteristics and ...

http://www.raritanval.edu/uploadedFiles/faculty/sci/part-time/Cleverley/Recombinant%20DNA%20extended.ppt

Date added: August 15, 2013 - Views: 18

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Genetic Engineering - Fremont Union High School District

... bacteria Recombinant plasmids Plasmids can be modified in biological labs Modified plasmid = Recombinant plasmid Plasmids can be used as cloning vectors to ...

http://web.fuhsd.org/pamela_chow/apbio/lectures/Protein%20Synthesis/Genetic%20Engineering.ppt

Date added: April 1, 2013 - Views: 42

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Genetic Engineering and Biotechnology - Morgan Park High School

Vectors for cloning 5. Using plasmids as vectors Isolating the gene from the gene source DNA is divided into genes (units of inheritance) ...

http://www.morganparkcps.org/ourpages/auto/2010/4/15/55162980/Genetic%20Engineering%20and%20Biotechnology.ppt

Date added: October 24, 2013 - Views: 4

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Gene Cloning

Fundamental of Biotechnology Plasmids

http://xa.yimg.com/kq/groups/24240295/972920507/name/MCS

Date added: January 17, 2014 - Views: 19

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Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

This enables the vectors to readily ligate DNA sequences with compatible ends ... Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2550.ppt

Date added: September 27, 2011 - Views: 458

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Biotechnology and Recombinant DNA - University of Nevada, Reno

Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.

http://wolfweb.unr.edu/homepage/borgeson/Bio251/10biotechnewa.ppt

Date added: July 28, 2013 - Views: 25

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PowerPoint (6.6 MB) - Department of Microbiology

Introduction to Cloning and Recombinant DNA Technology David Bedwell, Ph.D. ... Plasmids useful as cloning vectors must have: An origin of replication.

http://www.microbio.uab.edu/bedwell/Lectures/BioteachLecture6.24.09.ppt

Date added: October 4, 2011 - Views: 163

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Plasmid - KSU

3 Molecular cloning. Plasmids used in genetic engineering are called . vectors. Vector Element . Vector Element .

http://fac.ksu.edu.sa/sites/default/files/plasmid_lab_.pptx

Date added: November 16, 2014 - Views: 1

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Introduction to Vectors

Some plasmids are present in E. coli at 200-500 ... 2 Slide 3 Slide 4 Plasmids Vectors Plasmid Engineering Cloning Scheme Slide 9 Slide 10 DNA ...

http://faculty.pingry.org/domara/documents/WAKSMANLECTURE1_sept09.ppt

Date added: March 20, 2015 - Views: 1

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Chapter 13 An Introduction to Cloning and Recombinant DNA

Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...

http://www.bio.brandeis.edu/classes/heredity/Lecture%20Powerpoints/Chapter_13_1.ppt

Date added: August 2, 2013 - Views: 14

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Lambda Vectors and their replication - Department Of ...

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 36

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Chapter 13 Slides - people.Virginia.EDU

... new DNA fragments to the sticky ends of plasmid Cloning Vectors Plasmids that can be modified to carry new genes Plasmids useful as cloning vectors must ...

http://people.virginia.edu/~cmg/slides/chapter_13.ppt

Date added: March 3, 2012 - Views: 41

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Chapter 9 – DNA-Based Information Technologies

Chapter 9 – DNA-Based Information Technologies Recombinant DNA molecules are constructed with DNA from different sources Recombinant DNA molecules are created often ...

http://ww2.valdosta.edu/~dgosnell/3601/May%202005/DNA-Based%20Information%20Technologies.ppt

Date added: May 3, 2013 - Views: 18

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PowerPoint Presentation

BACs are maintained as single copy plasmids in E. coli and exclude other BAC plasmids from ... pBeloBAC11 represents the second generation BAC cloning vectors.

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 226

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Recombinant DNA Technology - NIU Department of Biological ...

Plasmids for cloning carry drug resistance genes that are used for selection. ... Most cloning vectors use a system for detecting the presence of a recombinant ...

http://www.bios.niu.edu/johns/bioinf_intro/Recombinant%20DNA%20Technology.ppt

Date added: May 8, 2012 - Views: 54

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Manipulating DNA - Arkansas State University

Manipulating DNA Recombinant DNA: a ... Cloning and vectors Traditional ... then delivered Plasmid vectors Plasmids are circular DNA molecules Plasmids can be cut ...

http://www.clt.astate.edu/dgilmore/Genetics/Powerpoints/Spring%2006/G12a%20Sp06.ppt

Date added: June 24, 2013 - Views: 4

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Recombinant DNA

Plasmids Serve As Vectors Plasmids are considered "replicons", ... Gene Cloning The second step of the genetic engineering process is gene cloning.

http://biology.uco.edu/personalpages/meeks/Micro%20Take%20Home%20Test%5CRecombinant%20DNA.ppt

Date added: May 10, 2014 - Views: 4

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Recombinant DNA Technology - NIU Department of Biological ...

Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 257

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CHAPTER 6

Cloning Vectors Cloning vectors are plasmids that can be modified to carry new ... End Chapter 12 Recombinant DNA: Cloning and Creation of Chimeric Genes ...

http://iweb.tntech.edu/tbanks/4610/GGch12web.ppt

Date added: August 23, 2011 - Views: 22

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Restriction Endonucleases and Plasmids

Restriction Endonucleases, Plasmids, and Transformation Restriction Endonucleases Enzymes purified from bacteria Protect bacterial cell from viruses by degrading ...

http://www.fevanet.com/chemistry/SBI4UO_WEB/restriction%20endonucleases_plasmid_transformation.ppt

Date added: November 8, 2014 - Views: 1

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Cloning and Sequencing - Winston-Salem/Forsyth County Schools ...

Cloning and Sequencing Project overview Background Project will have you cloning the gene that codes for the enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...

http://www.wsfcs.k12.nc.us/cms/lib/NC01001395/Centricity/Domain/3098/Biotech%20II/Unit%20C%20Gene%20Expression/Cloning%20and%20Sequencing.ppt

Date added: May 3, 2013 - Views: 35

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PowerPoint Presentation

Plasmids Small circular autonomously replicating extrachromosomal DNA Modified plasmids, called cloning vectors Are used by molecular biologists to isolate

http://bio.classes.ucsc.edu/bio105/Winter10/Lecture13/lect13.ppt

Date added: March 16, 2013 - Views: 30

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Recombinant DNA Technology - Ning

Recombinant DNA Technology Prof ... Bacteriophage λ Cosmid library Allows cloning of 45 ... the F plasmid and the lacZ region of pUC plasmids It’s a low ...

http://api.ning.com/files/nX3y*H5eFo02-NTyuh6T8nAZHjcQYESgMxNYxGzEwO*Vp8ZuRCGZ2JIrlPlFXMQyH0bfT4WCH0IVJ0Tc9E9gLPK00CYbF1hd/RecombinantDNATechnology.ppt

Date added: October 4, 2011 - Views: 167

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Gene Cloning

Lecture # 04 Cloning Vectors Recombinant DNA Technology Common General Cloning Strategy Target DNA from donor organism extracted, cut with compatible restriction ...

http://xa.yimg.com/kq/groups/24240295/1734630456/name/lecture+04.ppt

Date added: February 18, 2014 - Views: 12

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Molecular Cloning - Advances in Bioscience Education (ABE)

Plasmids – used in cloning small segments of DNA (10-15 kb) ... Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date: 6/9/2006 11:02:09 PM

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 83

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Biotechnology and Genetic Engineering-PBIO 450/550

Bacteria carrying recombinant plasmids therefore give rise to white ... of restriction enzyme sites Cloning vectors and their insert capacities Plasmid ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO4500%20Lecture2.ppt

Date added: May 2, 2013 - Views: 29

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2.

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 26

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Setting up a transformation--how will the competent cells be ...

... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Specialized cloning vectors expression vectors and tags vectors for large pieces of DNA, ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: November 15, 2011 - Views: 73