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Cloning into Plasmids - Buffalo State College...

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Cloning into Plasmids - Buffalo State College...

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 94

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Recombinant DNA - Egg Harbor Township Schools

DNA cloning yields multiple copies of a gene of other DNA segment-VOCAB. Derived from bacteria, these products are used to cut strands of DNA at specific locations

http://www.eht.k12.nj.us/~oneilj/NEURO_GENETICS_files/Genetics/Recombinant%20DNA_cloning_plasmids_vectors.pptx

Date added: April 17, 2014 - Views: 9

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Cloning - Sam Houston State University

Recombinant DNA Cont. Plasmids can carry up to 20,000 bp of foreign DNA. Besides bacterial plasmids, some other cloning vectors include viruses, bacteria artificial ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 27, 2013 - Views: 26

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16.2 In vivo gene cloning – the use of vectors -...

Inserting genes into Plasmids. ... which of course can be isolated and then introduced into bacterial cells via vectors. ... 16.2 In vivo gene cloning – the use of ...

http://vbio.weebly.com/uploads/2/8/2/4/2824362/16.2_-_in_vivo_gene_cloning_-_the_use_of_vectors.pptx

Date added: June 16, 2012 - Views: 33

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Types of Cloning Vectors

Types of Cloning Vectors. A. Plasmid Vectors. Plasmids are relatively small, circular DNA molecules. They are commonly used as vectors for DNA fragments up to

http://www.ut.edu.sa/documents/10156/3136643/Lecture+2

Date added: February 12, 2015 - Views: 2

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No Slide Title

Molecular Genetics Techniques BIT 220 Chapter 20 What is Cloning? Recombinant DNA technologies Restriction Endonucleases Why use REs? Plasmids Cloning Vectors Plasmid ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 76

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. 3. ... Plant Cloning Vectors.

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: May 26, 2012 - Views: 164

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Plasmid DNA isolation - Assiut University

Cloning vectors are DNA molecules that are used to "transport ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: January 25, 2015 - Views: 1

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 3

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Chapter 13 An Introduction to Cloning and...

Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...

http://www.bio.brandeis.edu/classes/heredity/Lecture%20Powerpoints/Chapter_13_1.ppt

Date added: August 2, 2013 - Views: 18

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Chapter 9 – DNA-Based Information Technologies

... Cutting with EcoRI Cloning vectors can be: plasmids, bacteriophages, viruses, small artificial chromosomes Vectors have at least one unique cloning site: ...

http://www.valdosta.edu/~dgosnell/3601/May%202005/DNA-Based%20Information%20Technologies.ppt

Date added: October 7, 2011 - Views: 47

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Plasmid - KSU

3 Molecular cloning. Plasmids used in genetic engineering are called . vectors. Vector Element . Vector Element .

http://fac.ksu.edu.sa/sites/default/files/plasmid_lab_.pptx

Date added: November 16, 2014 - Views: 3

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Recombinant DNA

Fact Sheet Describing Recombinant DNA and Elements Utilizing Recombinant DNA Such as Plasmids and Viral Vectors, ... methods Cloning Vectors ...

http://www.aryansexport.com/PPT/Recombinant%20DNA%20technology.ppt

Date added: June 26, 2014 - Views: 8

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Genetic Engineering - Fremont Union High School...

... bacteria Recombinant plasmids Plasmids can be modified in biological labs Modified plasmid = Recombinant plasmid Plasmids can be used as cloning vectors to ...

http://web.fuhsd.org/pamela_chow/apbio/lectures/Protein%20Synthesis/Genetic%20Engineering.ppt

Date added: April 1, 2013 - Views: 46

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Restriction Endonucleases and Plasmids

Restriction Endonucleases, Plasmids, and Transformation Restriction Endonucleases Enzymes purified from bacteria Protect bacterial cell from viruses by degrading ...

http://www.fevanet.com/chemistry/SBI4UO_WEB/restriction%20endonucleases_plasmid_transformation.ppt

Date added: November 8, 2014 - Views: 1

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Introduction to Vectors - Pingry School

Some plasmids are present in E. coli at 200-500 ... 2 Slide 3 Slide 4 Plasmids Vectors Plasmid Engineering Cloning Scheme Slide 9 Slide 10 DNA ...

http://faculty.pingry.org/domara/documents/WAKSMANLECTURE1_sept09.ppt

Date added: March 20, 2015 - Views: 3

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What is Gene Technology

Plasmids and Vectors Aims: Must be able to outline what plasmids and vectors are. Should be able to explain how plasmids and vectors are utilised in research.

http://manorlakesscience.wikispaces.com/file/view/Biology%204.13%20Plasmids%20and%20Vectors.ppt/515311918/Biology%204.13%20Plasmids%20and%20Vectors.ppt

Date added: March 26, 2015 - Views: 1

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Biotechnology and Recombinant DNA

Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.

http://wolfweb.unr.edu/homepage/borgeson/Bio251/10biotechnewa.ppt

Date added: July 28, 2013 - Views: 27

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Molecular Cloning I: Restriction Enzymes, PCR, ...

This enables the vectors to readily ligate DNA sequences with compatible ends ... Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and Gel Electrophoresis

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2550.ppt

Date added: September 27, 2011 - Views: 467

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Recombinant DNA Technology

Recombinant DNA Technology Prof ... Bacteriophage λ Cosmid library Allows cloning of 45 kb ... the F plasmid and the lacZ region of pUC plasmids It’s a low copy ...

http://api.ning.com/files/nX3y*H5eFo02-NTyuh6T8nAZHjcQYESgMxNYxGzEwO*Vp8ZuRCGZ2JIrlPlFXMQyH0bfT4WCH0IVJ0Tc9E9gLPK00CYbF1hd/RecombinantDNATechnology.ppt

Date added: October 4, 2011 - Views: 167

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Introduction to molecular cloning - ABE

Plasmids – used in cloning small segments of DNA (10-15 kb) ... Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date: 6/9/2006 11:02:09 PM

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 86

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PowerPoint Presentation

Plasmids are also known as cloning vectors. Cloning Vector – a DNA molecule that can carry foreign DNA into a host cell and replicate there. Examples of Gene Cloning.

http://intranet.oswego308.org/schools/uploads/files/70491/Chapter%2020%20-%20Biotechnology(1).pptx

Date added: May 1, 2015 - Views: 1

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2.

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 26

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Gene Cloning

Lecture # 04 Cloning Vectors Recombinant DNA Technology Common General Cloning Strategy Target DNA from donor organism extracted, cut with compatible restriction ...

http://xa.yimg.com/kq/groups/24240295/1734630456/name/lecture+04.ppt

Date added: February 18, 2014 - Views: 12

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PowerPoint Presentation

DNA-based information technologies Understand basics steps/enzymes/features of DNA cloning Know main types of cloning vectors used - pros & cons of each

http://chemistry.creighton.edu/%7Ejksoukup/DNAtech2_2010.ppt

Date added: February 27, 2014 - Views: 16

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Inquiry into Life Twelfth Edition - Regis...

4-Summary Basic Plasmid Cloning Vectors. pBR322 and the pUC plasmids. pBR322 has . 2 antibiotic resistance genes . Many unique restriction sites for inserting foreign DNA

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 2, 2013 - Views: 30

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Ch. 9: Presentation Slides - Tistory

The simplest types of vectors are plasmids whose DNA is double-stranded and circular. ... Cloning Vectors. Three types of vectors commonly used for cloning into E. coli:

http://www.pnumbl.tistory.com/attachment/[email protected]

Date added: October 31, 2014 - Views: 1

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Bacterial recombination- Transduction

... Plasmids can be cloning vectors Ch 8 pg 175 Plasmid requirements in biotech PowerPoint Presentation Viruses can bring new genes into a cell Transduction ...

http://www.mccc.edu/~blinderl/documents/Ch15_bacteriaslide_set2.ppt

Date added: March 29, 2015 - Views: 1

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PowerPoint Presentation

Cloning Vectors A) Bacterial Plasmids An Expression-Cloning Vector An Expression Vector for Making a LacZ Fusion Protein LacZ can be replaced with: 1) ...

http://www-rci.rutgers.edu/%7Emolbio/Courses/MBB_408_512/Lec18b.ppt

Date added: April 8, 2015 - Views: 1

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Ligation and Transformation - ISU Plant Genome...

... amplification of gene product A type of cloning vector used to ... of vectors GFP keeps its ... Picture Transformation Plasmids Overall ...

http://www.plantgenomeoutreach.eeob.iastate.edu/teachmod/Bacterial_Transformation.ppt

Date added: October 14, 2011 - Views: 62

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DNA Technology - Monroe Township School District

DNA Technology Chapter 13 Lab Biology 1A Chapter 15 Lab biology 2b Chapter 13 Honors Biology 4A

http://www.monroe.k12.nj.us/cms/lib07/NJ01000268/Centricity/Domain/270/DNA%20Technology.ppt

Date added: September 13, 2013 - Views: 49

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Chapter 9 Molecular Genetic Techniques and...

Chapter 9 Molecular Genetic Techniques and Genomics Presented by: Troy Coke What and Why? What?: A gene of interest is inserted into another organism, enabling it to ...

http://biology.uco.edu/bidlack/cellphys/doc/powerpoint%20s2004/Recombinant%20DNA.ppt

Date added: August 2, 2013 - Views: 4

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What is gene clonning?

Plasmids almost always carry one or more genes, ... Several eukaryotic viruses have been employed as cloning vectors for specialized applications: for example,

http://www.fapgent.org/uploads/1/8/9/7/18975273/cpt_1-2.pptx

Date added: May 4, 2015 - Views: 1

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Library construction - Center for Bioinformatics...

A plasmid vector for cloning Plasmid vectors Expression vector How to clone DNA ... Blue/White Color Screening Selecting Colonies with Recombinant Plasmids ...

http://www.cbcb.umd.edu/confcour/CMSC828H-materials/Cloning-into-plasmids.ppt

Date added: February 12, 2013 - Views: 4

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Biotechnology and Genetic Engineering-PBIO 450/550

Cloning vectors Eukaryotic gene ... The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in rep of pMB1; (2) bla ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB720Lecture211.ppt

Date added: May 14, 2013 - Views: 9

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Biotechnology and Genetic Engineering-PBIO 450/550

Cloning vectors Eukaryotic gene ... Bacteria carrying recombinant plasmids therefore give rise to white colonies. pGEM-3Z Cloning foreign DNA into a plasmid vector ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture208.ppt

Date added: October 6, 2011 - Views: 41

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PowerPoint Presentation

DNA ligase splices pieces into plasmids or other cloning vectors How to form recombinant DNA?

http://skywalker.cochise.edu/ashraft/web/BIO101ppt/What%20is%20biotechnology.ppt

Date added: November 8, 2014 - Views: 2

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VOCABULARY for GENETIC TECHNOLOGY - Baker High...

VOCABULARY for GENETIC TECHNOLOGY CLONING Produces an ... Plasmids Plasmids are small rings of DNA in bacteria ... Transgenic organisms Plasmids VECTORS ...

http://www.bville.org/tfiles/folder196/VOCABULARY%20for%20GENETIC%20TECHNOLOGY%2Eppt

Date added: May 13, 2013 - Views: 9

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Recombinant DNA and Genetic Engineering

Use bacterial cloning vectors ... 7_Concourse Studying and Manipulating Genomes Genetic Engineering Molecular Toolkit Making Recombinant DNA Using Plasmids ...

http://ww2.harford.edu/faculty/rbeahm/chapter11.ppt

Date added: February 26, 2015 - Views: 5

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Chapter 3 Methods in Molecular Biology and Genetic...

Methods in Molecular Biology and Genetic Engineering

http://teacherweb.com/CA/SolanoCommunityCollege/EdwardRe/XXXXX-Biosci-2-Spring-2015-Introduction-to-Molecular-Biology-XXXXX.ppt

Date added: March 29, 2015 - Views: 1

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5.2.3. Genetic Engineering - Mrs Miller's Blog

Genetic Engineering. define the term recombinant DNA; ... Bacterial Cells and Plasmids. Most vectors are bacteria. DNA cut with restriction enzyme then placed in vector.

https://mrsmillersblog.files.wordpress.com/2010/09/a2-5-2-3-genetic-engineering.pptx

Date added: February 7, 2015 - Views: 3

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DNA Technology - NCSSM Links

DNA Technology Overview DNA ... DNA vectors, and host organisms Either plasmids or bacteriophages can ... gene cloning results when the foreign genes replicate inside ...

http://courses.ncssm.edu/mccoy/DNA%20Technology.ppt

Date added: October 30, 2011 - Views: 79

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Recombinant DNA

Chapter 7 Recombinant DNA Technology and Genomics

http://mydsn.net/docs-tools/BIO205/3rdIng07.ppt

Date added: August 25, 2014 - Views: 2

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Biocatalysis - Moodle

Cloning Vectors Allow Amplification of Inserted DNA Segments. Plasmids ... Bacterial artificial chromosomes (E.coli) 3. Vector from yeasts . Plasmids-circular DNA, ...

http://moodle.chatham.edu/mod/resource/view.php?id=147629

Date added: March 27, 2015 - Views: 1

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DNA Technology

Cloning vectors: carrier used to ... Plasmids are used to clone a gene so that bacteria will produce a specific protein. Example: Insulin. Diagram: Transplanting ...

http://hs.pleasantvilleschools.enschool.org/ourpages/auto/2012/8/22/53773241/DNA%20Technology.pptx

Date added: May 2, 2015 - Views: 1

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DNA Technology & Gene Mapping

DNA Cloning. Most techniques for cloning make use of bacteria (E. coli) as they have small circular plasmids that are receptive for insertion of foreign DNA.

http://santasusana.org/pakelly/DNA%20Technology%20&%20Gene%20Mapping.pptx

Date added: March 24, 2014 - Views: 1

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Ch. 20 Biotechnology

DNA cloning yields multiple copies of a gene or other DNA segment. Cloning vectors include plasmids and bacterial artificial chromosomes (BACs). Recombinant plasmids ...

http://www.alemany.org/apps/download/rbFpXhLWFijorgAJME7ey4hPooYrKBHRCYO5CSJJuOmd1N8K.pptx/ch20AP.pptx

Date added: April 24, 2015 - Views: 1

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Chapter 15 The Techniques of Molecular Genetics

... (phage and plasmids) ... different cloning vectors must be used in different species. Special cloning vectors can replicate in other prokaryotes and in eukaryotes ...

http://www2.fiu.edu/~barbieri/CH14.ppt

Date added: December 26, 2013 - Views: 14

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PowerPoint Presentation

The cloning process (1 ... conjugation genes oriT: origin of transfer PLASMIDS: APPLICATIONS • Cloning vectors • Gene expression • Transposon delivery ...

http://www.ibt.unam.mx/computo/pdfs/canovas/dos.ppt

Date added: February 11, 2013 - Views: 24