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Initiation: DNA replication begins at specific sites on the double helix referred to as origins of replication. ... The Results Found that when the bacteria has been infected with T2 phages containing labeled protein, the radioactivity ended up in the liquid but not bacteria.
Date added: November 27, 2013 - Views: 18
Brief History Many people contributed to our understanding of DNA T.H. Morgan (1908) Frederick Griffith (1928) Avery ... Protein coat labeled with 35S DNA labeled with 32P bacteriophages infect bacterial cells T2 bacteriophages are labeled with radioactive isotopes S vs. P bacterial ...
Date added: January 29, 2012 - Views: 43
Shaped like a double helix (spiral staircase) Base-pairing rules (from Chargaff) that adenine always bonds to thymine and cytosine always bonds to guanine. ... The end of each side of the DNA molecule that has the “free” phosphate is labeled 5’ and the other end is labeled 3’.
Date added: June 5, 2013 - Views: 16
... These experiments told Avery that DNA is the hereditary material in cells Hershey-chase experiment Hershey and Chase labeled two ... Rosalind Franklin Use x-ray crystallography to determine that DNA is a double helix This lead to the structure that Watson and Crick used Watson and ...
Date added: August 1, 2013 - Views: 1
* * Watson & Crick’s model was inspired by 3 recent ... Protein coat labeled with 35S DNA labeled with 32P bacteriophages infect bacterial cells T2 bacteriophages are labeled with radioactive isotopes S vs. P bacterial cells are agitated to remove viral protein coats 35S radioactivity ...
Date added: March 22, 2012 - Views: 17
... forming a double helix LE 16-7 Watson and Crick built models of a double helix to conform to the X-rays and ... conservative model and the dispersive model LE 16-10 Experiments by Meselson and Stahl supported the semiconservative model They labeled the nucleotides of the old ...
Date added: August 23, 2011 - Views: 69
... (England) Built the 1st accurate 3D (tin and wire) model of DNA “Double Helix” – spiral staircase Purine is always ... Propose an experiment to determine how DNA replication occurs 1950’s Meselson & Stahl Cultured Ecoli on medium labeled w/ 15N nt Transferred EColi to medium ...
Date added: April 3, 2014 - Views: 1
... entitled "The Double Helix," depicts Franklin as an underling ... Protein coat labeled with 35S DNA labeled with 32P bacteriophages infect bacterial cells T2 bacteriophages are labeled with radioactive isotopes S vs. P bacterial cells are agitated to remove viral protein coats 35S ...
Date added: September 7, 2013 - Views: 9
Do this on the back of WB 34 Structure of DNA Double stranded helix Deoxyribose sugar ... Using a venn diagram, compare and contrast codons to anticodons ... Analysis question 2 Draw a labeled diagram of your DNA molecule. Keep the illustration in a straight “ladder” form as ...
Date added: October 30, 2011 - Views: 68
... one or more nucleotide pairs are inserted into the DNA double helix Deletion mutation ... Radioactively labeled the DNA core and protein capsid of a phage Results indicated that DNA, not the protein, ...
Date added: May 3, 2013 - Views: 11
Chapter 11 DNA and Protein Synthesis Mrs. Svencer ... bacteria Batch 1 Labeled protein coats with radioactive sulfur Radioactivity detected outside of the cells Batch 2 Labeled DNA with radioactive phosphorus Radioactivity inside cells Therefore, ...
Date added: October 16, 2013 - Views: 8
Watson and Crick discovered the double helix by building models to conform to X-ray data By the beginnings of the 1950’s, ... When they examined the bacterial cultures with T2 phage that had radio-labeled DNA, ...
Date added: November 18, 2013 - Views: 1
... The Structure of DNA ... phosphorus was used to label the [DNA / protein] in the viruses. 11. Hershey and Chase discovered that after the 32P-labeled ... DNA structure a. a five-carbon sugar b. type of weak bond between base pairs that holds the double helix together c. four kinds ...
Date added: January 24, 2014 - Views: 7
Phage infects E. coli bacteria. Phages are labeled with radioactive material. Adhere to the phosphorus of the DNA molecule and the sulfur of the protein coat. ... Crick Main Features The Double Helix A History Lesson ...
Date added: February 28, 2014 - Views: 6
DNA: THE Genetic Material Section1 ... DNA at rates as high as 1000 nucleotides per seconBecause each of the two daughters of a dividing cell inherits a new DNA double helix containing ... would become part of the phages’ DNA Step 2 Labeled phages used to infect two separate batches ...
Date added: November 15, 2011 - Views: 74
... phage were labeled with either . 35. S to label proteins. or . 32. P to label DNA. phage were incubated with bacteria to allow infection, ... the accepted model for the structure of the DNA double helix was published by James Watson and Francis Crick in 1953.
Date added: October 25, 2014 - Views: 1
Then they allowed protein labeled and DNA-labeled phage to separately infect E. coli bacteria. 3. They removed the phage coats from the cells in a blender. ... evaluate the contributions of Franklin and Wilkins in helping Watson and Crick discover DNA’s double helix structure. 2.
Date added: April 23, 2014 - Views: 1
Griffith’s Mouse Experiment Fredrick ... Infected E. coli bacteria with two types of labeled T2 4. 32P is discovered within the bacteria ... 45.0 Aythya americana 25.8 25.8 24.2 24.2 48.4 * Structure of DNA James D. Watson/Francis H. Crick 1953 proposed the Double Helix Model based on two ...
Date added: July 13, 2012 - Views: 44
... Glucose Ribose Deoxyribose Lactose In the DNA double helix, adenine always matches thymine because: Adenine is polar and thymine ... (blue) Radioactive protein (yellow) Conclusion: Infected bacteria are labeled with radioactive phosphorus but not with radioactive sulfur, supporting ...
Date added: January 19, 2014 - Views: 3
... Watson and Crick proposed that DNA is a double helix Griffith Discovers Transformation 1928 Attempting to develop a vaccine ... Consist of protein and DNA Inject their hereditary material into bacteria Hershey & Chase’s Experiments Created labeled bacteriophages Radioactive ...
Date added: August 10, 2013 - Views: 4
DNA Double Helix Double Helix Nucleotides A Winding Staircase, continued The nitrogen base in a ... What is the function of the structure labeled A? A. separating DNA strands B. reconnecting DNA strands C. adding nucleotides to make new DNA strands D. checking the new DNA strands for ...
Date added: December 21, 2013 - Views: 6
_____ were responsible for the discovery of the double helix structure of DNA. ... Draw Neat labeled diagrams of. Structure of DNA. DNA Replication. In double stranded DNA how do the four bases (C,A,T,G) pair up with each other.
Date added: May 3, 2013 - Views: 15
Double Helix. DNA, RNA, and Protein Synthesis . Discovery of DNA. ... They radioactively labeled the DNA and the protein (this allowed them to trace the path of each) They found DNA was injected into the bacteria to infect it, not protein.
Date added: February 5, 2014 - Views: 3
... Base Pairing to a Template Strand The relationship between structure and function is manifest in the double helix Since the two strands of ... Semiconservative replication Conservative replication Dispersive replication Meselson-Stahl Experiments Labeled the nucleotides of old ...
Date added: October 17, 2011 - Views: 34
The Composition and Structure of DNA and RNA 7a. The DNA Double Helix 7b. The DNA Double Helix Chargaff ... (Figure 2.5). 4.2. In one part of the experiment, T2 proteins were labeled with 35S, and in the other part, T2 DNA was labeled with 32P. Then each group of labeled viruses was mixed ...
Date added: May 19, 2013 - Views: 15
... James Watson and Francis Crick introduced an elegant double-helical model for the structure of deoxyribonucleic acid, ... Experiments by Matthew Meselson and Franklin Stahl supported the semiconservative model They labeled the nucleotides of the old strands with a heavy isotope of ...
Date added: May 28, 2013 - Views: 9
Watson and Crick discovered the double helix by building models to conform to X-ray data ... When they examined the bacterial cultures with T2 phage that had radio-labeled DNA, most of the radioactivity was in the pellet with the bacteria.
Date added: May 9, 2012 - Views: 15
... A = 30.9% T = 29.4% G = 19.9% C = 19.8% Structure of DNA Watson & Crick developed double helix model of DNA other ... Alfred Hershey Martha Chase Protein coat labeled with 35S DNA labeled with 32P bacteriophages infect bacterial cells T2 bacteriophages are labeled with ...
Date added: December 27, 2012 - Views: 19
Chapter 10 The Structure, Replication and Repair of DNA Key Questions How did biologists discover what genes were made of and what they did? What is the structure of DNA?
Date added: August 10, 2014 - Views: 1
... labeled virus DNA with phosphorus Protein coat DNA BACTERIOPHAGE Conclusion: ... forming a double helix G and C are complementary as are A and T The two strands of DNA run in opposite directions (antiparallel) DNA is Complementary: ...
Date added: May 19, 2013 - Views: 5
Chapter 12: Molecular Genetics ... entitled "The Double Helix," depicts Franklin as an underling ... Protein coat labeled with 35S DNA labeled with 32P bacteriophages infect bacterial cells T2 bacteriophages are labeled with radioactive isotopes S vs. P bacterial cells are agitated to remove ...
Date added: September 7, 2011 - Views: 100
Fig. 2.11 Chemical structures of DNA and RNA The Discovery of the DNA Double Helix 1. James Watson and Francis Crick published the famous double-helix structure in 1953. ... The 35S-labeled protein was found outside the infected cells, while the 32P-labeled DNA was inside the E. coli, ...
Date added: June 4, 2012 - Views: 30
Called a double helix -two strands bonded together & twisted The bases form the rungs of the ladder ... C-G Sugars and phosphates form sides of ladder. The carbons in the sugar are labeled DNA Animation http://www.johnkyrk.com/DNAanatomy.html http://126.96.36.199/pub/flash/24/menu ...
Date added: September 1, 2013 - Views: 8
... Watson and Crick proposed that DNA is a double helix Structure of Nucleotides in DNA Each nucleotide consists of Deoxyribose ... (blue) being injected into bacterium 35S remains outside cells virus particle labeled with 32P DNA (blue) ...
Date added: August 8, 2013 - Views: 6
... replication always proceeds in the 5’ to 3’ direction The strands in the double helix are ... viral proteins remained outside the host cell during infection Radioactively-labeled (phosphorus) viral DNA traveled inside the host cell -the injected DNA molecules cause the cells to to ...
Date added: September 27, 2013 - Views: 10
A 4-nucleotide-long segment of DNA. (arbitrary choice of bases) Only variation comes from nitrogenous base side chains ...
Date added: August 24, 2014 - Views: 1
DNA Structure: Double Helix DNA ... Dideoxy Method Each band on the gel is one base longer than the previous band Each didyoxynucleotide is labeled by different fluorescent dye G, black; A, green; T, red; C, purple As each band comes off the bottom of the gel, ...
Date added: October 15, 2011 - Views: 18
The Hammerling Experiment: ... viruses that attack bacteria They employed the bacteriophage T2 which is a DNA virus They labeled the viral DNA with radioactive isotope of phosphorus 32P and the protein coat with radioactive sulfur 35S After the labeled viruses were allowed to infect the ...
Date added: October 24, 2011 - Views: 19
The Watson and Crick DNA Double helix The correct structure of DNA was first deduced by J.D. Watson and F.H.C.Crick in 1953. ... Autoradiography is particularly useful in studying DNA metabolism because DNA can be specifically labeled by growing cells on [H3]thymidine, ...
Date added: August 25, 2011 - Views: 35
The arrows mark replication bubbles; the diameters of DNA chain in both arms of these bubbles indicate that they are double-stranded. 17. Priming DNA synthesis DNA polymerases can extend a chain but cannot start a chain. ... The DNA has been labeled with 3H-deoxythymidine, ...
Date added: August 1, 2013 - Views: 14
Question of the Day DEC 20 A nucleotide does not contain A. a 5-C sugar B. polymerase C. a nitrogenous base D. a phosphate group
Date added: April 22, 2014 - Views: 38
Parent cell First replication Second replication Experiments by Meselson and Stahl supported the semiconservative model They labeled the nucleotides of the old strands with a heavy isotope of nitrogen, ...
Date added: September 7, 2012 - Views: 42
Griffith’s Transformation Experiment Avery’s Transformation Experiment Hershey-Chase Bacteriophage Experiment Tobacco Mosaic Virus (TMV) Experiment Nucleotides - composition and structure Double-helix model of DNA ... Infected E. coli bacteria with two types of labeled T2 4. 32P is ...
Date added: October 24, 2011 - Views: 29