Download Cosmids Ppt from our fatest mirrorCombined Cosmids (ppt) - University of Illinois at Chicago - UIC
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Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153
Date added: October 24, 2011 - Views: 50
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 208
Cosmids are extracted from bacteria and mixed with restriction endonucleases. Cleaved cosmids are mixed with foreign DNA that has been cleaved with the same endonuclease.
Date added: July 18, 2013 - Views: 35
Cosmids - Plasmids with phage packaging sites p. 2-16 4. YACs - Yeast Artificial Chromosomes p. 2-17 Vector Insert Size Vector Type Cloned DNA (Kb) Plasmid 20 Phage ...
Date added: November 12, 2011 - Views: 109
Principles of cloning, vectors and cloning strategies DNA CLONING DNA cloning is a technique for reproducing DNA fragments. It can be achieved by two different approaches: cell based using polymerase chain reaction (PCR).
Date added: January 23, 2013 - Views: 33
Cosmids, however, always form colonies and not plaques. Also the clone density is much lower with around 105 - 106 CFU per µg of ligated DNA.
Date added: May 21, 2012 - Views: 48
... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti (causes crown gall tumors). Plasmid codes for virulence gene and regulates pant growth ...
Date added: August 2, 2013 - Views: 12
... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic library in YACs * Look at vector first, Where are all the the sites that you know about? *
Date added: August 8, 2014 - Views: 1
... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs PowerPoint Presentation PowerPoint Presentation Shotgun sequencing Chromosome Walking Chromosome jumping. Southern (DNA) and ...
Date added: October 12, 2011 - Views: 17
Other vectors include bacteriophage, cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation Selection for cells carrying recombinant plasmids by plating cells on media with antibiotic.
Date added: January 13, 2013 - Views: 16
Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle Gun We can insert the gene into cells – Now what? Basic Steps ...
Date added: May 3, 2013 - Views: 22
Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. Bacterial Artificial Chromosomes (BACs): Circular DNA molecules used for cloning very long segments of genomic DNA. Bacterial ...
Date added: October 7, 2011 - Views: 106
... by PCR to determine inheritence patterns Produce linkage map with nearby loci Physical Maps Radiation Hybrid/YACs/Cosmids Restriction Sites Sequence Tagged Sites 100 Kb resolution needed 30,000 STS’s Expressed Sequence Tags Detection PCR Hybridization FISH Fluoresecent in situ ...
Date added: November 6, 2011 - Views: 92
Contain other features similar to plasmids/cosmids such as origin sequence and polylinker. Recombinant DNA Libraries (2 major types): Genomic/chromosomal library, ...
Date added: October 2, 2014 - Views: 1
... 8-20 kb Cosmids - an extrachromosomal circular DNA molecule that combines features of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) - based on bacterial mini-F plasmids. cloning limit: ...
Date added: December 2, 2011 - Views: 92
... allow reproduction of gene plasmids phages cosmids part viral, part plasmid BACs - bacterial artificial chromosomes YACs - yeast artificial chromosomes Cloning Cloning Use SAME restriction endonuclease to cut out target gene and open vector Must use an enzyme that produces sticky ends!
Date added: March 10, 2012 - Views: 24
... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a generic, minimal plasmid origin of replication antibiotic resistance restriction site for cloning pBi430/530 1500 base pairs ...
Date added: October 3, 2011 - Views: 93
Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs (Bacterial artificial chromosomes) Large low copy number plasmids (have ori and ...
Date added: October 16, 2011 - Views: 189
... Transformation (via bacterial mechanisms); plasmids BACS YACS Transduction (via virus mechanisms); phage cosmids Cloning Step 3 ...vectors contain selectable markers, only cells that contain vector DNA will survive selection, ...
Date added: October 6, 2011 - Views: 136
... from GeneScan and Ensembl are occasionally contradictory chicken also contained small mini-chromosomes that resemble cosmids which were lumped into the category of ChrUn, ...
Date added: May 6, 2013 - Views: 7
Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) from bacteriophage λ used to clone larger fragments of up to 45 Kb Bacteriophage λ ...
Date added: November 5, 2011 - Views: 80
Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation format
Date added: February 13, 2013 - Views: 31
1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, easy to handle Straightforward selection strategies Useful for cloning small DNA fragments (< 10kbp) Disadvantages: Less useful for cloning large DNA fragments ...
Date added: December 10, 2013 - Views: 10
Hence, the cosmids can not be selected with G418 upon transfection into mammalian cells. CLONING SITES: VECTOR: (1) Sort tubes are spun at 3 K rpm for 2 hours at 4 degrees Celsius. (2) The resulting pellet is extracted with Proteinase K/SDS; ...
Date added: October 20, 2013 - Views: 4
Troubleshooting DNA Sequences: Guidelines and Suggestions Sequencing Instruments: AB 3100-Avant, 3130XL both Capillary Based Advantages Higher throughput Can reinject samples Higher separation efficiency Better resolution No Plates!
Date added: July 26, 2013 - Views: 18
Cosmids- are hybrid vectors of l phage and plasmids. They can replicate their DNA in the cell with a plasmid and be packaged like a phage. They can insert up to 50kb. 4. Yeast artificial chromosomes (YAC)- primarily used in genome sequencing projects.
Date added: September 30, 2013 - Views: 31
Genome Sequencing Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs (bp).
Date added: September 12, 2011 - Views: 49
... DNA splicing * * * * * * * Table 3.1 * * * Table 3.1 * * Figure 3.3b * * * Figure 3.3b * * Figure 3.5 * * * Figure 3.5 Cosmids Contain cos ends of lambda DNA, a plasmid origin of replication, and genes for antibiotic resistance, ...
Date added: June 30, 2014 - Views: 4
... lambda (~ 15-20 kb) cosmids for larger DNA molecules (~ 40-50 kb) BAC: bacterial artificial chromosome (~ 100-300 kb) YAC: yeast artificial chromosome (~ 1000 kb) The vector is cut with the restriction endonuclease and mixed with the DNA restriction fragments to be cloned.
Date added: May 3, 2013 - Views: 19
... A. Rajandream Central Veterinary Lab. R.G. Hewinson S.V. Gordon NIH NIAID * Sequence obtained from combined approach cosmids, BA Cs and WGS 4000 genes; 60% with functions; islands with 80%GC 1 Polyketides involved in 3 diff pathologies Produced by PKS, ...
Date added: November 14, 2011 - Views: 25
... Replication as Single-Stranded Phage DNA BACs and PACs are vectors that can carry much larger fragments of DNA than cosmids because they do not have packaging constraints Slide 15 Slide 16 Eukaryotic and Shuttle Vectors An E. coli-Yeast Shuttle Vector Yeast Artificial Chromosomes ...
Date added: July 1, 2014 - Views: 3
... to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...
Date added: October 6, 2011 - Views: 24
... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages Molecular Cloning: Cloning Vectors 2. Bacteriophages: example is lambda 1/3 of genome ...
Date added: May 6, 2013 - Views: 2
Cosmids can accept even larger inserts—up to 50 kb—making them a favorite choice for genomic libraries. The second major class of phage vector is composed of the M13 phages.
Date added: January 28, 2012 - Views: 161
The physical map of the C. elegans genome was put together after ordering then sequencing genomic YACs and cosmids. * The genetic and physical maps can be aligned with each other. The spacing can vary between genetic markers and their physical genes; however, ...
Date added: June 27, 2012 - Views: 17
... 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) - based on bacterial mini-F plasmids. cloning limit: 150-300 kb (350kb in some books).
Date added: February 18, 2014 - Views: 9
Use these phage to infect new E. coli. Cosmids are similar to phage vectors: use lambda, but remove all but the ends (cos sites), ori, and selectable marker. Package in vitro--becomes a large (50 kb) ...
Date added: October 7, 2011 - Views: 250
Je continue maintenant avec mon deuxieme point concernant l immunogenicite. Sequence obtained from combined approach cosmids, BA Cs and WGS 4000 genes; 60% with functions; ...
Date added: August 31, 2013 - Views: 10
... phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning - PCR Requires primers, thermostable DNA polymerase Extreme sensitivity is asset and a problem ...
Date added: November 28, 2013 - Views: 3
Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Konkuk university Created Date: 1/20/2002 6:56:56 PM Document presentation format
Date added: May 2, 2013 - Views: 27
Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid
Date added: May 2, 2013 - Views: 19
... Created a cosmid library from the YAC clone Cosmid subclones used to screen a cDNA library of human fetal brain RNA The cosmids hybridized to a portion of a gene designated as FMR1 Pedigree of a family with the Fragile X mutation segregating FMR1 Expression Northern Blot of FMR1 was done ...
Date added: August 12, 2014 - Views: 1
The gene is inserted into a vector (plasmid, virus, cosmids) that will insert the DNA into a cloning host. Cloning host is usually bacterium or yeast that can replicate the gene and translate it into a protein product.
Date added: December 13, 2011 - Views: 80
H3 Cosmids, YACs and BACs — Cloning large DNA fragments Analysis of eukaryotic genes and the genome organization of eukaryotes requires vectors with a larger capacity for cloned DNA than plasmids or phage Human genome (3 x 109 bp ...
Date added: February 13, 2012 - Views: 10