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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153

http://www.uic.edu/depts/mcmi/faculty/silver/Combined%20Cosmids%20Map.ppt

Date added: October 24, 2011 - Views: 50

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 214

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Vectors - College of Computer, Mathematical, and Natural Sciences

Most vectors are genetically engineered plasmids or phages. There are also cosmid vectors, ... Cosmids combine essential elements of a plasmid and Lambda systems.

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Date added: July 18, 2013 - Views: 42

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 2

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Plasmids and Vectors - Molecular Biology for Secondary ...

A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs ...

http://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: October 16, 2011 - Views: 196

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PowerPoint Presentation

Central Dogma of Biology DNA RNA Protein

http://www.molecularbiology.yolasite.com/resources/Lecture%202.ppt

Date added: October 13, 2014 - Views: 3

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PowerPoint Presentation

Vectors for large DNA inserts: Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b-lactamase: breaks down ampicillin ...

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 24

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DNA cloning intro.ppt - Biology

BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 34

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Justc/courses/RecombDNA/Week6. Ppt

Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, deleting insert DNA (to reduce drag on cells) ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week6.ppt

Date added: October 7, 2011 - Views: 65

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PowerPoint Presentation

... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 12

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PowerPoint Presentation

Cosmids can be used to build genomic libraries. Cosmids are able to contain 37 to 52 kb of DNA, while normal plasmids are able to carry only 1–20 kb.

http://forest.mtu.edu/classes/fw3300/BL3300%202011/2011%20%20Chapt%2003%20091904%20cpj.ppt

Date added: May 21, 2012 - Views: 53

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PowerPoint Presentation

Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 109

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PowerPoint Presentation

... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...

http://www.pascack.k12.nj.us/cms/lib5/NJ01000238/Centricity/Domain/142/WSSP06-Ch2-LIbraries.ppt

Date added: August 8, 2014 - Views: 2

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Lecture 16: Genomics - University of Alabama at Birmingham

The Human Genome Project Mapping and Sequencing the Genomes of Model Organisms Data Collection and ... 200 Kb Intermediate Cosmids Lambda clones Small ...

http://www.genome.uab.edu/MIC753/files/16_Genome.ppt

Date added: November 6, 2011 - Views: 93

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 93

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PowerPoint Presentation

Chromosome walking can ... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs ...

http://genemol.org/molecular-bio/technics.ppt

Date added: October 12, 2011 - Views: 18

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Restriction Enzymes, Vectors, and Genetic Libraries

Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 23

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PowerPoint Presentation

Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures-2013/chapter_08.ppt

Date added: October 2, 2014 - Views: 2

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PowerPoint Presentation

... the target gene Cloning Vectors segments of DNA that carry target gene; allow reproduction of gene plasmids phages cosmids part viral, part plasmid BACs ...

http://people.uncw.edu/doddd/Biology%20335/PowerPoints/DNAtech.ppt

Date added: March 10, 2012 - Views: 25

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PowerPoint Presentation

... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 19

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PowerPoint Presentation - DNA Libraries - Biology Department ...

... (via virus mechanisms); phage cosmids Cloning Step 3 ...vectors contain selectable markers, only cells that contain vector DNA will survive selection, ...

http://www.biol.wwu.edu/young/321/lectures/lg8.ppt

Date added: October 6, 2011 - Views: 140

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PowerPoint Presentation

DNA polymerases--making copies, adding labels, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a generic, ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 97

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Genome Sequencing - Kennesaw State University College of ...

Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs ... a set of cDNA sequences to a set of cosmids, ...

http://science.kennesaw.edu/~jsalern3/Site/bioinfo/Genome%20Sequencing.ppt

Date added: September 12, 2011 - Views: 53

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PowerPoint Presentation

... and manipulated mainly at DNA level. 1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, ...

http://faculty.ksu.edu.sa/73614/amustafa/355/chapter3.ppt

Date added: December 10, 2013 - Views: 11

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Molecular Cloning - University of Hawaii

Foreign DNA Host organism Vector DNA for cloning Means of inserting foreign DNA into the vector Method of placing ... (10-15 kb) Cosmids – plasmids ...

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 83

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PowerPoint Presentation

... from GeneScan and Ensembl are occasionally contradictory chicken also contained small mini-chromosomes that resemble cosmids which were lumped into the ...

http://instructional1.calstatela.edu/jmomand2/2004/presentations/ppt/ng.ppt

Date added: May 6, 2013 - Views: 7

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PowerPoint Presentation

... lambda (~ 15-20 kb) cosmids for larger DNA molecules (~ 40-50 kb) BAC: bacterial artificial chromosome (~ 100-300 kb) YAC: yeast artificial chromosome ...

http://site.iugaza.edu.ps/fsharif/files/2010/02/MB-62.ppt

Date added: May 3, 2013 - Views: 22

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Biotechnology and Genetic Engineering-PBIO 450/550

MCB 7200: Molecular Biology Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library construction Screening a ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 34

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Présentation PowerPoint - Institut Pasteur

... A. Rajandream Central Veterinary Lab. R.G. Hewinson S.V. Gordon NIH NIAID * Sequence obtained from combined approach cosmids, BA Cs and WGS 4000 genes; ...

http://www.pasteur.fr/~tekaia/BCGA/TALKS/Cole_Origin_Tuberculosis.ppt

Date added: November 14, 2011 - Views: 25

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DNASem5.11.07.ppt - The University of Vermont

... LOR scores for three different approaches BAC’s: Default set-up and AutoSeq1 Cosmids, Bacs, Genomic: BAC’s: Modified Set-up, BAC profile BAC ...

http://www.uvm.edu/medicine/vtcancercenter/documents/DNASem5.11.07.ppt

Date added: July 26, 2013 - Views: 20

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Lambda Vectors and their replication - Department Of ...

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 33

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Gene Cloning

... cloning limit: 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) ...

http://xa.yimg.com/kq/groups/24240295/1734630456/name/lecture+04.ppt

Date added: February 18, 2014 - Views: 12

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Slide 1

... 19-42 Please note that the neo marker only contains a prokaryotic promoter. Hence, the cosmids can not be selected with G418 upon transfection into mammalian cells.

https://biosci-batzerlab.biology.lsu.edu/Hum_Mol_Gen_Lectures/Hum_Mol_Genet_Lecture_3_January_27_2009.ppt

Date added: September 17, 2014 - Views: 2

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Chapter 4 Molecular Cloning Methods - XMU

Chapter 4 Molecular Cloning Methods Introduction The significance of gene cloning To elucidate the structure and function of genes. i.e.: investigating hGH gene hGH ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 164

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Recombinant DNA Technology - NIU Department of Biological ...

Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 252

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Chapter 14

after insertion of foreign DNA the recombinant phage genome is packaged into the capsid and used to infect host cells Cosmids do not exist in nature these vectors ...

http://novellaqalive2.mheducation.com/sites/dl/premium/0072992913/instructor/339222/chapt14_lecture.ppt

Date added: November 23, 2014 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

... to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 26

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Section D - Prokaryotic and Eukaryotic Chromosome Structure

H3 Cosmids, YACs and BACs — Cloning large DNA fragments Analysis of eukaryotic genes and the genome organization of eukaryotes requires vectors with a ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 11

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Foundations in Microbiology - South Georgia College

Lecture PowerPoint to accompany Foundations in Microbiology Sixth Edition Talaro Chapter 10 Genetic Engineering: A Revolution in Molecular Biology

http://faculty.sgc.edu/sundram/2215/Chapter10_lecture.ppt

Date added: December 13, 2011 - Views: 83

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No Slide Title

... phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...

http://web.bio.utk.edu/humangenetics/lecture/molec_tools06sep02.ppt

Date added: November 28, 2013 - Views: 4

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Chem 360 Lecture slides

Recombinant DNA Technology

http://www.weston.org/schools/ms/biologyweb/heredity/handouts/recombinant_dna_tech_ppt.ppt

Date added: May 2, 2013 - Views: 39

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Slide 1

... Cosmids, Phagemids; YAC and BAC vectors, Model vectors for eukaryotes – Viruses, Unit II Restriction modification, enzymes used in recombinant DNA technology ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_3.ppt

Date added: July 1, 2014 - Views: 4

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PowerPoint Presentation

PowerPoint Presentation

http://yahool.weebly.com/uploads/1/1/3/2/11323392/cdna.ppt

Date added: November 8, 2014 - Views: 2

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No Slide Title

... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 3

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PowerPoint Presentation

... library of clones 10kb library of clones 150kb library of clones * C. Elegans 100Mb Drosophila 120Mb Human 3,200Mb Cosmids, ...

http://www.columbia.edu/cu/biology/courses/w3034/2010/Dan/ppt/Biotech%20Topic3(10).ppt

Date added: November 23, 2014 - Views: 1

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Inquiry into Life Twelfth Edition - McGraw-Hill Education

... labeled nucleic acid with sequence matching the gene of interest Cosmids Cosmids are designed for cloning large DNA fragments Behave as plasmid and phage ...

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 24

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Présentation PowerPoint

Je continue maintenant avec mon deuxieme point concernant l immunogenicite. Sequence obtained from combined approach cosmids, BA Cs and WGS 4000 genes; ...

http://www.pasteur.fr/~tekaia/BCGA/TALKS/Cole_Evol_Pathogenic_Mycobact.ppt

Date added: August 31, 2013 - Views: 10

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Fragile X Syndrome - University of Wisconsin–Eau Claire

Fragile X Syndrome (Martin ... Created a cosmid library from the YAC clone Cosmid subclones used to screen a cDNA library of human fetal brain RNA The cosmids ...

http://people.uwec.edu/anderju/biol409/presentations/Fragile%20X%20Syndrome.ppt

Date added: August 12, 2014 - Views: 1

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The Small RNA Chaperone Hfq and Multiple Small RNAs Control ...

Title: The Small RNA Chaperone Hfq and Multiple Small RNAs Control Quorum Sensing in Vibrio harveyi and Vibrio cholerae Author: pafink Last modified by

http://jevisick.faculty.noctrl.edu/465/presentations/JC10.ppt

Date added: May 24, 2014 - Views: 7