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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153

http://www.uic.edu/depts/mcmi/faculty/silver/Combined%20Cosmids%20Map.ppt

Date added: October 24, 2011 - Views: 51

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 225

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 4

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Plasmids and Vectors - Molecular Biology for Secondary ...

A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: January 31, 2015 - Views: 1

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Vectors - College of Computer, Mathematical, and Natural Sciences

Most vectors are genetically engineered plasmids or phages. There are also cosmid vectors, ... Cosmids combine essential elements of a plasmid and Lambda systems.

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Date added: July 18, 2013 - Views: 44

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PowerPoint Presentation

Cosmids can be used to build genomic libraries. Cosmids are able to contain 37 to 52 kb of DNA, while normal plasmids are able to carry only 1–20 kb.

http://forest.mtu.edu/classes/fw3300/BL3300%202011/2011%20%20Chapt%2003%20091904%20cpj.ppt

Date added: May 21, 2012 - Views: 57

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Restriction Enzymes, Vectors, and Genetic Libraries

Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 32

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Justc/courses/RecombDNA/Week6. Ppt

Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, deleting insert DNA (to reduce drag on cells) ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week6.ppt

Date added: October 7, 2011 - Views: 73

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PowerPoint Presentation

Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://mercury2.iab.uaf.edu/kevin_mccracken/genetics/lectures/chapter_08.ppt

Date added: August 31, 2013 - Views: 4

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PowerPoint Presentation

... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 14

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DNA cloning intro.ppt - Biology

BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 34

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PowerPoint Presentation

... Cosmids PACs BACs Bacteriophage Vectors Lambda: Each clone can accept inserts of ~15-20 kb. Replace part of l DNA that is not needed for replication with ...

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: January 4, 2015 - Views: 1

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Lecture 16: Genomics - University of Alabama at Birmingham

Genomics The Human Genome Project Mapping and Sequencing the Genomes of Model Organisms Data Collection and Distribution Ethical, Legal, and Social Considerations ...

http://www.genome.uab.edu/MIC753/files/16_Genome.ppt

Date added: November 6, 2011 - Views: 95

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PowerPoint Presentation

Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 111

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PowerPoint Presentation

Fundamentals of Mapping and Sequencing Basic principles and procedures of mapping and DNA sequencing

http://forest.mtu.edu/faculty/joshi/new/BL3300%202006/BL3300%202006%20slides/chapter%203/03%20091904%20cpj.ppt

Date added: October 19, 2013 - Views: 7

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PowerPoint Presentation

Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function

http://public.gettysburg.edu/~hiraizum/bio211/0909.ppt

Date added: September 8, 2014 - Views: 1

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PowerPoint Presentation

... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 20

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PowerPoint Presentation

Chromosome walking can ... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs ...

http://genemol.org/molecular-bio/technics.ppt

Date added: October 12, 2011 - Views: 19

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Cloning into Plasmids - Buffalo State College Faculty and ...

... 8-20 kb Cosmids ... Fragment into Vector Multi Cloning Site The pUC 18 or 19 Cloning Vector RE Sites in blue occur only once in the plasmid Lac Z α ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 93

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PowerPoint Presentation - DNA Libraries - Biology Department ...

... construct a cDNA library, isolation of hemoglobin clones is facilitated, genomic: ... (via virus mechanisms); phage cosmids Cloning Step 3 ...

http://www.biol.wwu.edu/young/321/lectures/lg8.ppt

Date added: October 6, 2011 - Views: 144

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Biotechnology and Genetic Engineering-PBIO 450/550

Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 37

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PowerPoint Presentation

Central Dogma of Biology DNA RNA Protein

http://www.molecularbiology.yolasite.com/resources/Lecture%202.ppt

Date added: October 13, 2014 - Views: 3

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PowerPoint Presentation

... library of clones 10kb library of clones 150kb library of clones * C. Elegans 100Mb Drosophila 120Mb Human 3,200Mb Cosmids, ...

http://www.columbia.edu/cu/biology/courses/w3034/2010/Dan/ppt/Biotech%20Topic3(10).ppt

Date added: November 23, 2014 - Views: 1

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Molecular Cloning - Advances in Bioscience Education (ABE)

Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids ... Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date:

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 83

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PowerPoint Presentation

Mobilizing DNA: vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs * 5´ ... G^A A T T C ... 3´ 3´ ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 99

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Recombinant DNA Technology for the non-science major.

Cosmids (artificial constructions) A prokaryotic vector should: 1. Be capable ... Recombinant DNA Technology for the non-science major. Author: Janey Last modified by:

http://cogs.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: October 16, 2012 - Views: 30

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PowerPoint Presentation

... from GeneScan and Ensembl are occasionally contradictory chicken also contained small mini-chromosomes that resemble cosmids which were lumped into the ...

http://instructional1.calstatela.edu/jmomand2/2004/presentations/ppt/ng.ppt

Date added: May 6, 2013 - Views: 7

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Genome Sequencing - Kennesaw State University College of ...

Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs ... a set of cDNA sequences to a set of cosmids, ...

http://science.kennesaw.edu/~jsalern3/Site/bioinfo/Genome%20Sequencing.ppt

Date added: September 12, 2011 - Views: 56

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PowerPoint Presentation

... and manipulated mainly at DNA level. 1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, ...

http://faculty.ksu.edu.sa/73614/amustafa/355/chapter3.ppt

Date added: December 10, 2013 - Views: 14

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PowerPoint Presentation

... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...

http://www.pascack.k12.nj.us/cms/lib5/NJ01000238/Centricity/Domain/142/WSSP06-Ch2-LIbraries.ppt

Date added: August 8, 2014 - Views: 3

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Lambda Vectors and their replication - Department Of ...

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 36

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DNASem5.11.07.ppt - The University of Vermont

... LOR scores for three different approaches BAC’s: Default set-up and AutoSeq1 Cosmids, Bacs, Genomic: BAC’s: Modified Set-up, BAC profile BAC ...

http://www.uvm.edu/medicine/vtcancercenter/documents/DNASem5.11.07.ppt

Date added: July 26, 2013 - Views: 23

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450/550 Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 27

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Part II: Recombinant DNA Technology.ppt - Dr.J.Rajesh Banu Ph ...

Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Konkuk university Created Date: 1/20/2002 6:56:56 PM Document presentation format

http://rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: May 2, 2013 - Views: 53

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cDNA Library - MB210 Microbial Biotechnology - MB

... vectors used in library construction Plasmids Up to 10kb inserts Modified Lambda phage Up to 20kb inserts/40kbp for cosmids Artificial cloning vectors BAC ...

http://mb206.wikispaces.com/file/view/MB206_fhs_Int_011_AT_Jan09.ppt

Date added: December 29, 2012 - Views: 95

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PowerPoint Presentation

... lambda (~ 15-20 kb) cosmids for larger DNA molecules (~ 40-50 kb) BAC: bacterial artificial chromosome (~ 100-300 kb) YAC: yeast artificial chromosome ...

http://site.iugaza.edu.ps/fsharif/files/2010/02/MB-62.ppt

Date added: May 3, 2013 - Views: 26

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Inquiry into Life Twelfth Edition - Innovative Learning ...

... labeled nucleic acid with sequence matching the gene of interest Cosmids Cosmids are designed for cloning large DNA fragments Behave both as plasmid and phage ...

http://highered.mheducation.com/sites/dl/free/0073525324/891545/Chapter_04_lecture.ppt

Date added: July 10, 2014 - Views: 2

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Slide 1

Hence, the cosmids can not be selected with G418 upon transfection into mammalian cells. CLONING SITES: VECTOR: SORTED CHROMOSOME ISOLATION PROCEDURE (1) ...

https://biosci-batzerlab.biology.lsu.edu/Hum_Mol_Gen_Lectures/Hum_Mol_Genet_Lecture_3_February_1_2011.ppt

Date added: May 7, 2013 - Views: 5

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No Slide Title

... phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...

http://web.bio.utk.edu/humangenetics/lecture/molec_tools06sep02.ppt

Date added: November 28, 2013 - Views: 4

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Foundations in Microbiology - South Georgia College

... excised by restriction endonucleases, and isolated. The gene is inserted into a vector (plasmid, virus, cosmids) that will insert the DNA into a cloning host.

http://faculty.sgc.edu/sundram/2215/Chapter10_lecture.ppt

Date added: December 13, 2011 - Views: 92

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Recombinant DNA Technology - NIU Department of Biological ...

Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 257

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Forward Genetics - University of Colorado Boulder

Forward Genetics Survey ... mor-2 sup-23 evl-7 Genetic map map unit Cloned genes Non-cloned genes mDf4 nDf41 rme-2 unc-5 fem-1 skn-1 Physical map cosmids YACs ...

http://www.colorado.edu/MCDB/HanLab/part2.baisc_forward_genetics.ppt

Date added: September 24, 2011 - Views: 17

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PowerPoint Presentation

... cosmids (40 Kbp) known dist ~500 bp ~500 bp ARACHNE: Whole Genome Shotgun Assembly 1. Find overlapping reads 4. Derive consensus sequence ..ACGATTACAATAGGTT..

http://www.upch.edu.pe/facien/fc/dbmbqf/zimic/cursos/bioinfo%202008/clases/20%20prediccion%20de%20genes.ppt

Date added: March 9, 2015 - Views: 1

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No Slide Title

... P1cloning systems derived from bacteriophage P1 one of the primary tools of E. coli geneticists for many years like cosmids, ...

http://blumberg-serv.bio.uci.edu/past%20teaching/bio203-w2003/bio203-lecture%2018.ppt

Date added: March 2, 2015 - Views: 1

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Chem 360 Lecture slides - Weston

Cosmids - artificially ... Radiolabeled vs. fluorescent tag Isolation of Gene of Interest Isolation of Gene of Interest Use of Antibodies Ab ppt protein Protein being ...

http://www.weston.org/schools/ms/biologyweb/heredity/handouts/recombinant_dna_tech_ppt.ppt

Date added: May 2, 2013 - Views: 43

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Inquiry into Life Twelfth Edition - Innovative Learning ...

Inquiry into Life Twelfth Edition ... l Phage Vectors Phage Vector Advantages Cloning Using a Phage Vector Genomic Libraries Plaque Hybridization Cosmids ...

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 28

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Foundations of Biology

... but not in the middle Cosmids A cosmid is a plasmid with the l phage cos site included in it The cos site signals for packaging into l phage, ...

http://grisda.org/resources/Power_Points/Molecular_Genetics/Fly_Genome.ppt

Date added: December 10, 2013 - Views: 3

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Section D - Prokaryotic and Eukaryotic Chromosome Structure

H3 Cosmids, YACs and BACs — Cloning large DNA fragments Analysis of eukaryotic genes and the genome organization of eukaryotes requires vectors with a ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 12

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Finding Disease Genes - NIU Department of Biological Sciences

Need to clone the region between these genes walking by cosmids over 500 kbp problem with uncloneable repeated sequences: needed to jump over them.

http://www.bios.niu.edu/johns/humgen/Finding_Disease_Genes.ppt

Date added: June 28, 2012 - Views: 25