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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153

http://www.uic.edu/depts/mcmi/faculty/silver/Combined%20Cosmids%20Map.ppt

Date added: October 24, 2011 - Views: 51

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 224

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Vectors - College of Computer, Mathematical, and Natural Sciences

Most vectors are genetically engineered plasmids or phages. There are also cosmid vectors, ... Cosmids combine essential elements of a plasmid and Lambda systems.

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Date added: July 18, 2013 - Views: 44

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 2, 2013 - Views: 4

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DNA cloning intro.ppt - Biology

BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 34

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justc/courses/RecombDNA/Week6. ppt

Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, deleting insert DNA (to reduce drag on cells) ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week6.ppt

Date added: October 7, 2011 - Views: 68

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PowerPoint Presentation

Central Dogma of Biology DNA RNA Protein

http://www.molecularbiology.yolasite.com/resources/Lecture%202.ppt

Date added: October 13, 2014 - Views: 3

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PowerPoint Presentation

... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 14

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PowerPoint Presentation

Cosmids can be used to build genomic libraries. Cosmids are able to contain 37 to 52 kb of DNA, while normal plasmids are able to carry only 1–20 kb.

http://forest.mtu.edu/classes/fw3300/BL3300%202011/2011%20%20Chapt%2003%20091904%20cpj.ppt

Date added: May 21, 2012 - Views: 55

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Restriction Enzymes, Vectors, and Genetic Libraries

Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 28

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PowerPoint Presentation

Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://mercury2.iab.uaf.edu/kevin_mccracken/genetics/lectures/chapter_08.ppt

Date added: August 31, 2013 - Views: 3

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PowerPoint Presentation

Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 110

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PowerPoint Presentation

Vectors for large DNA inserts: Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b-lactamase: breaks down ampicillin ...

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 25

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Cloning into Plasmids - Buffalo State College Faculty and ...

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 93

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PowerPoint Presentation

... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs ...

http://genemol.org/molecular-bio/technics.ppt

Date added: October 12, 2011 - Views: 19

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PowerPoint Presentation

... the target gene Cloning Vectors segments of DNA that carry target gene; allow reproduction of gene plasmids phages cosmids part viral, part plasmid BACs ...

http://people.uncw.edu/doddd/Biology%20335/PowerPoints/DNAtech.ppt

Date added: March 10, 2012 - Views: 26

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Lecture 16: Genomics - University of Alabama at Birmingham

The Human Genome Project Mapping and Sequencing the Genomes of Model Organisms Data Collection and ... 200 Kb Intermediate Cosmids Lambda clones Small ...

http://www.genome.uab.edu/MIC753/files/16_Genome.ppt

Date added: November 6, 2011 - Views: 95

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PowerPoint Presentation

... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 20

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PowerPoint Presentation

DNA polymerases--making copies, adding labels, ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a generic, ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 98

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PowerPoint Presentation

Fundamentals of Mapping and Sequencing Basic principles and procedures of mapping and DNA sequencing

http://forest.mtu.edu/faculty/joshi/new/BL3300%202006/BL3300%202006%20slides/chapter%203/03%20091904%20cpj.ppt

Date added: October 19, 2013 - Views: 7

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PowerPoint Presentation - DNA Libraries - Biology Department ...

... construct a cDNA library, isolation of hemoglobin clones is facilitated, genomic: ... (via virus mechanisms); phage cosmids Cloning Step 3 ...

http://www.biol.wwu.edu/young/321/lectures/lg8.ppt

Date added: October 6, 2011 - Views: 142

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Biotechnology and Genetic Engineering-PBIO 450/550

MCB 7200: Molecular Biology Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library construction Screening a ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 35

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PowerPoint Presentation

... from GeneScan and Ensembl are occasionally contradictory chicken also contained small mini-chromosomes that resemble cosmids which were lumped into the ...

http://instructional1.calstatela.edu/jmomand2/2004/presentations/ppt/ng.ppt

Date added: May 6, 2013 - Views: 7

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Lambda Vectors and their replication - Department Of ...

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 36

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Molecular Cloning - Advances in Bioscience Education (ABE)

Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences ... used in cloning larger segments of DNA ...

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 83

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PowerPoint Presentation

... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...

http://www.pascack.k12.nj.us/cms/lib5/NJ01000238/Centricity/Domain/142/WSSP06-Ch2-LIbraries.ppt

Date added: August 8, 2014 - Views: 2

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Plasmids and Vectors - Molecular Biology for Secondary ...

A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs ...

http://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: October 16, 2011 - Views: 200

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PowerPoint Presentation

... and manipulated mainly at DNA level. 1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, ...

http://faculty.ksu.edu.sa/73614/amustafa/355/chapter3.ppt

Date added: December 10, 2013 - Views: 13

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Slide 1

... 19-42 Please note that the neo marker only contains a prokaryotic promoter. Hence, the cosmids can not be selected with G418 upon transfection into mammalian cells.

https://biosci-batzerlab.biology.lsu.edu/Hum_Mol_Gen_Lectures/Hum_Mol_Genet_Lecture_3_January_27_2009.ppt

Date added: September 17, 2014 - Views: 2

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Genome Sequencing - Kennesaw State University College of ...

Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs ... a set of cDNA sequences to a set of cosmids, ...

http://science.kennesaw.edu/~jsalern3/Site/bioinfo/Genome%20Sequencing.ppt

Date added: September 12, 2011 - Views: 56

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450/550 Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 26

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Chapter 4 Molecular Cloning Methods

Chapter 4 Molecular Cloning Methods Introduction The significance of gene cloning To elucidate the structure and function of genes. i.e.: investigating hGH gene hGH ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: January 28, 2012 - Views: 168

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Présentation PowerPoint - Institut Pasteur | Pour la ...

... A. Rajandream Central Veterinary Lab. R.G. Hewinson S.V. Gordon NIH NIAID * Sequence obtained from combined approach cosmids, BA Cs and WGS 4000 genes; ...

http://www.pasteur.fr/~tekaia/BCGA/TALKS/Cole_Origin_Tuberculosis.ppt

Date added: November 14, 2011 - Views: 25

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DNASem5.11.07.ppt

... LOR scores for three different approaches BAC’s: Default set-up and AutoSeq1 Cosmids, Bacs, Genomic: BAC’s: Modified Set-up, BAC profile BAC ...

http://www.uvm.edu/medicine/vtcancercenter/documents/DNASem5.11.07.ppt

Date added: July 26, 2013 - Views: 22

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Recombinant DNA Technology - NIU Department of Biological ...

Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 254

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Slide 1

... Cosmids, Phagemids; YAC and BAC vectors, Model vectors for eukaryotes – Viruses, Unit II Restriction modification, enzymes used in recombinant DNA technology ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_3.ppt

Date added: July 1, 2014 - Views: 4

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Section D - Prokaryotic and Eukaryotic Chromosome Structure

H3 Cosmids, YACs and BACs — Cloning large DNA fragments Analysis of eukaryotic genes and the genome organization of eukaryotes requires vectors with a ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 11

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Foundations in Microbiology - South Georgia College

Lecture PowerPoint to accompany Foundations in Microbiology Sixth Edition Talaro Chapter 10 Genetic Engineering: A Revolution in Molecular Biology

http://faculty.sgc.edu/sundram/2215/Chapter10_lecture.ppt

Date added: December 13, 2011 - Views: 89

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PowerPoint Presentation

PowerPoint Presentation ... Recombinant DNA

http://site.iugaza.edu.ps/fsharif/files/2010/02/MB-62.ppt

Date added: May 3, 2013 - Views: 24

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No Slide Title

... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: May 6, 2013 - Views: 3

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Chem 360 Lecture slides - Weston

Recombinant DNA Technology

http://www.weston.org/schools/ms/biologyweb/heredity/handouts/recombinant_dna_tech_ppt.ppt

Date added: May 2, 2013 - Views: 43

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: May 2, 2013 - Views: 20

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No Slide Title

Each of the 1,920 cosmids were tested for resistance to phagocytosis. 19 clones showed no growth on the assay plates. 13 cosmids showed resistance to phagocytosis ...

http://files.figshare.com/1477212/Lexi_Poster.ppt

Date added: January 4, 2015 - Views: 1

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Slide 1

... Cosmids can be packaged into lambda capsids and then manipulated as a phage Can also exist in the cell like a plasmid Can be used to clone very large pieces ...

http://classes.biology.ucsd.edu/bimm120.WI07/genetics2_doran_fa06.ppt

Date added: September 7, 2012 - Views: 29

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No Slide Title

Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3. Artificial Chromosomes YAC (yeast artificial Chromosomes - 500 ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: March 14, 2012 - Views: 75

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Forward Genetics - University of Colorado Boulder

Forward Genetics Survey Mutagenesis ... mor-2 sup-23 evl-7 Genetic map map unit Cloned genes Non-cloned genes mDf4 nDf41 rme-2 unc-5 fem-1 skn-1 Physical map cosmids ...

http://www.colorado.edu/MCDB/HanLab/part2.baisc_forward_genetics.ppt

Date added: September 24, 2011 - Views: 17

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Inquiry into Life Twelfth Edition - Innovative Learning ...

Inquiry into Life Twelfth Edition ... l Phage Vectors Phage Vector Advantages Cloning Using a Phage Vector Genomic Libraries Plaque Hybridization Cosmids ...

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: July 14, 2014 - Views: 26

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Inquiry into Life Twelfth Edition - Innovative Learning ...

... labeled nucleic acid with sequence matching the gene of interest Cosmids Cosmids are designed for cloning large DNA fragments Behave both as plasmid and phage ...

http://highered.mheducation.com/sites/dl/free/0073525324/891545/Chapter_04_lecture.ppt

Date added: July 10, 2014 - Views: 2

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Présentation PowerPoint - Institut Pasteur | Pour la ...

Je continue maintenant avec mon deuxieme point concernant l immunogenicite. Sequence obtained from combined approach cosmids, BA Cs and WGS 4000 genes; ...

http://www.pasteur.fr/~tekaia/BCGA/TALKS/Cole_Evol_Pathogenic_Mycobact.ppt

Date added: August 31, 2013 - Views: 10