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Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153
Date added: October 24, 2011 - Views: 52
Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...
Date added: August 2, 2013 - Views: 4
... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti ...
Date added: August 2, 2013 - Views: 14
... Cosmids PACs BACs Bacteriophage Vectors Lambda: Each clone can accept inserts of ~15-20 kb. Replace part of l DNA that is not needed for replication with ...
Date added: January 4, 2015 - Views: 1
... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...
Date added: January 13, 2013 - Views: 21
... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...
Date added: August 8, 2014 - Views: 3
Chromosome walking can ... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs ...
Date added: October 12, 2011 - Views: 19
... 8-20 kb Cosmids ... Fragment into Vector Multi Cloning Site The pUC 18 or 19 Cloning Vector RE Sites in blue occur only once in the plasmid Lac Z α ...
Date added: December 2, 2011 - Views: 94
Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function
Date added: September 8, 2014 - Views: 1
Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids ... Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date:
Date added: November 5, 2011 - Views: 86
Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation ...
Date added: February 13, 2013 - Views: 37
Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs ... a set of cDNA sequences to a set of cosmids, ...
Date added: September 12, 2011 - Views: 59
... of 2 million plus reads derived from low copy BACs defined by Dr. Tabata et al. 3) identification of low copy cosmids (US) ...
Date added: April 24, 2015 - Views: 1
Central Dogma of Biology DNA RNA Protein
Date added: October 13, 2014 - Views: 3
Biotechnology and Genetic Engineering PBIO 450/550 Gene libraries cDNA libraries Library screening Eukaryotic gene organization enhancers silencers Genomic library ...
Date added: October 6, 2011 - Views: 27
... phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...
Date added: November 28, 2013 - Views: 4
Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3. Artificial Chromosomes YAC (yeast artificial Chromosomes - 500 ...
Date added: March 14, 2012 - Views: 76
... but not in the middle Cosmids A cosmid is a plasmid with the l phage cos site included in it The cos site signals for packaging into l phage, ...
Date added: December 10, 2013 - Views: 3
... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...
Date added: May 6, 2013 - Views: 3
... library of clones 10kb library of clones 150kb library of clones * C. Elegans 100Mb Drosophila 120Mb Human 3,200Mb Cosmids, ...
Date added: November 23, 2014 - Views: 1
... vectors used in library construction Plasmids Up to 10kb inserts Modified Lambda phage Up to 20kb inserts/40kbp for cosmids Artificial cloning vectors BAC ...
Date added: December 29, 2012 - Views: 95
Recombinant DNA Andy Howard Introductory Biochemistry 18 November 2008
Date added: May 12, 2013 - Views: 5
Genome Characterization Sequencing, cont’d Physical Mapping
Date added: November 12, 2014 - Views: 1
Recombinant DNA Andy Howard Introductory Biochemistry 14 October 2010
Date added: August 19, 2013 - Views: 6
Title: PowerPoint Presentation Author: Wenun Last modified by: Wenun Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show
Date added: May 31, 2013 - Views: 2
... various cosmids localized to chromosome 4. b) Lanes 2-14: cosmids localized to chromosomes other than 4. The positive control in lanes 1 and 15 is cosL7L DNA . ...
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... Sequence these mapped clones (typically by the shotgun method). Path that was used for genome sequencing YACs BACs or Cosmids m13, plasmid map (MBP) map ...
Date added: August 24, 2014 - Views: 6
Viral Vectors May 8 Viral vectors ... cell-cell spread suppression of class I MHC promoter pA pack HSV-ori bacterial plasmid seq cloning site defective virus cosmids ...
Date added: February 19, 2012 - Views: 39
Title: Basic Molecular Biology Information Author: t80maj1 Last modified by: t80maj1 Created Date: 8/21/2006 4:36:06 PM Document presentation format
Date added: October 29, 2011 - Views: 74
Date added: June 22, 2012 - Views: 47
Bacteriophage 3. Cosmids B. Vectors for Other Organisms 1. Yeast Artificial Chromosomes (YACs) 2. Bacterial Artificial Chromosomes (BACs) 3. Plant Cloning ...
Date added: December 5, 2013 - Views: 10
Whole Genome Shotgun Sequencing cut many times at random genome forward-reverse linked reads plasmids (2 – 10 Kbp) cosmids (40 Kbp) ...
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... Progress to date: 105 of 319 cosmids fully processed 11493 independent insertions 10459 insertions in 2520 orfs (of 7825 in total) 4.2 ...
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Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of chromosomes ...
Date added: May 3, 2013 - Views: 1
6.095/6.895 - Computational Biology: Genomes, Networks, Evolution Putting Together Alignments & Comparing Assemblies Michael Brudno Department of Computer Science
Date added: October 16, 2013 - Views: 3
Capturing User Intent for Information Retrieval Hien Nguyen University of Connecticut Major advisor: Dr. Eugene Santos Jr. (UCONN) Associate advisor: Dr. Robert ...
Date added: July 3, 2014 - Views: 1
Lambda RED Recombination JIC in Norwich University of Tübingen
Date added: May 15, 2013 - Views: 2
Title: PowerPoint Presentation Author: Wenun Last modified by: buuic Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show (4:3)
Date added: September 15, 2012 - Views: 2
... potentially adjacent contigs and wrongly assembled ones. Use both. Make a cosmid library and sequence the positive cosmids.
Date added: April 10, 2015 - Views: 1
Basic Molecular Biology Many slides by Omkar Deshpande
Date added: April 8, 2015 - Views: 1
... bacteriophages (l and M13) (噬菌体) plasmid-bacteriophage l hybrids (cosmids) (考斯质粒－质粒和噬菌体杂和体). Yeast cloning vector: yeast ...
Date added: November 11, 2011 - Views: 13
... (Cloning Vectors) 噬菌體與 cosmids 舉例 轉殖載體 (Cloning Vectors) 植物基因轉殖與 Ti 質體 轉殖載體 (Cloning Vectors) ...
Date added: March 29, 2012 - Views: 2
Vectors for different situations. Vectors for Bacterial Cells . Plasmid Vecors. Bacteriophage Vectors . Cosmids. Phagemids. Yeast Cloning Vectors. The 2μ Circle
Date added: August 2, 2013 - Views: 5
Plasmids, viral DNA, Cosmids etc. Plamids : Plasmids are small, circular, double stranded extrachrosomal DNA present in bacterial cells. 3.
Date added: September 21, 2014 - Views: 1
FISH 세개의 sequenced DAZ cosmids를 FISH를 위한 probes로 이용하였음. 두개의 연결된 DAZ 유전자의 5번 말단 부위에 위치한 Cosmid 18E8 ...
Date added: September 18, 2012 - Views: 7
... cloning limit: 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) ...
Date added: February 18, 2014 - Views: 12
Some Terminology insert a fragment that was incorporated in a circular genome, and can be copied (cloned) vector the circular genome (host) that
Date added: December 1, 2014 - Views: 1
Common vectors used in libraries Plasmids Up to 10kbp inserts Modified Lambda phage Up to 20kbp inserts/40kbp for cosmids Artificial cloning vectors BAC- Vectors ...
Date added: February 8, 2014 - Views: 13