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Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153
Date added: October 24, 2011 - Views: 50
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 205
Title: Vectors Author: Warner Miller Last modified by: Mazzocchi Created Date: 1/27/2002 4:12:33 AM Document presentation format: On-screen Show Other titles
Date added: July 18, 2013 - Views: 34
Cosmids, however, always form colonies and not plaques. Also the clone density is much lower with around 105 - 106 CFU per µg of ligated DNA.
Date added: May 21, 2012 - Views: 46
Cosmids - Plasmids with phage packaging sites p. 2-16 4. YACs - Yeast Artificial Chromosomes p. 2-17 Vector Insert Size Vector Type Cloned DNA (Kb) Plasmid 20 Phage ...
Date added: November 12, 2011 - Views: 108
Other vectors include bacteriophage, cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation Selection for cells carrying recombinant plasmids by plating cells on media with antibiotic.
Date added: January 13, 2013 - Views: 14
... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti (causes crown gall tumors). Plasmid codes for virulence gene and regulates pant growth ...
Date added: August 2, 2013 - Views: 12
... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic library in YACs * Look at vector first, Where are all the the sites that you know about? *
Date added: August 8, 2014 - Views: 1
Chromosome walking can be used from the new jump position (in either direction ... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs PowerPoint Presentation PowerPoint Presentation Shotgun ...
Date added: October 12, 2011 - Views: 17
... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs a generic, minimal plasmid origin of replication antibiotic resistance restriction site for cloning pBi430/530 1500 base pairs ...
Date added: October 3, 2011 - Views: 90
Title: Lecture 16: Genomics Author: Elliot Lefkowitz Last modified by: Elliot Lefkowitz Created Date: 3/26/1998 5:35:50 PM Document presentation format
Date added: November 6, 2011 - Views: 86
Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle Gun We can insert the gene into cells – Now what? Basic Steps ...
Date added: May 3, 2013 - Views: 22
... thus the mRNA is enriched for hemoglobin sequences, construct a cDNA library, isolation of hemoglobin clones is facilitated, genomic: ~1 of ... (via bacterial mechanisms); plasmids BACS YACS Transduction (via virus mechanisms); phage cosmids Cloning Step 3 ...vectors contain ...
Date added: October 6, 2011 - Views: 131
Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. Bacterial Artificial Chromosomes (BACs): Circular DNA molecules used for cloning very long segments of genomic DNA. Bacterial ...
Date added: October 7, 2011 - Views: 104
Plasmids (Objectives of this Lecture) Understand the basic structures of plasmids Know the types of plasmids and their forms Undertand the methods used to transform E coli
Date added: May 3, 2013 - Views: 53
... from GeneScan and Ensembl are occasionally contradictory chicken also contained small mini-chromosomes that resemble cosmids which were lumped into the category of ChrUn, ...
Date added: May 6, 2013 - Views: 7
... allow reproduction of gene plasmids phages cosmids part viral, part plasmid BACs - bacterial artificial chromosomes YACs - yeast artificial chromosomes Cloning Cloning Use SAME restriction endonuclease to cut out target gene and open vector Must use an enzyme that produces sticky ends!
Date added: March 10, 2012 - Views: 20
Principles of cloning, vectors and cloning strategies DNA CLONING DNA cloning is a technique for reproducing DNA fragments. It can be achieved by two different approaches: cell based using polymerase chain reaction (PCR).
Date added: January 23, 2013 - Views: 31
1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, easy to handle Straightforward selection strategies Useful for cloning small DNA fragments (< 10kbp) Disadvantages: Less useful for cloning large DNA fragments ...
Date added: December 10, 2013 - Views: 9
Cosmids- are hybrid vectors of l phage and plasmids. They can replicate their DNA in the cell with a plasmid and be packaged like a phage. They can insert up to 50kb. 4. Yeast artificial chromosomes (YAC)- primarily used in genome sequencing projects.
Date added: September 30, 2013 - Views: 29
... DNA splicing * * * * * * * Table 3.1 * * * Table 3.1 * * Figure 3.3b * * * Figure 3.3b * * Figure 3.5 * * * Figure 3.5 Cosmids Contain cos ends of lambda DNA, a plasmid origin of replication, and genes for antibiotic resistance, ...
Date added: June 30, 2014 - Views: 4
... lambda (~ 15-20 kb) cosmids for larger DNA molecules (~ 40-50 kb) BAC: bacterial artificial chromosome (~ 100-300 kb) YAC: yeast artificial chromosome (~ 1000 kb) The vector is cut with the restriction endonuclease and mixed with the DNA restriction fragments to be cloned.
Date added: May 3, 2013 - Views: 17
The physical map of the C. elegans genome was put together after ordering then sequencing genomic YACs and cosmids. * The genetic and physical maps can be aligned with each other. The spacing can vary between genetic markers and their physical genes; however, ...
Date added: June 27, 2012 - Views: 15
... whose region can be replaced with foreign DNA without disrupting its life cycle; cloning limit: 8-20 kb Cosmids - an ... Fragment into Vector Multi Cloning Site The pUC 18 or 19 Cloning Vector RE Sites in blue occur only once in the plasmid Lac Z α Transformation and Selection ...
Date added: December 2, 2011 - Views: 89
Genome Sequencing Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs (bp).
Date added: September 12, 2011 - Views: 47
Title: Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date: 6/9/2006 11:02:09 PM Document presentation format: On-screen Show
Date added: November 5, 2011 - Views: 77
Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Sherry Fuller-Espie Created Date: 1/20/2002 6:56:56 PM Document presentation format
Date added: October 7, 2011 - Views: 246
N=ln(1-0.99)/ln(1-20kb/2.8 x 106kb)= 6.4 x 105 clones The first step in making a cDNA library: Purification of polyadenylated mRNA using oligo(dT)-cellulose Note: selection of the proper source (organ, ... and cosmids Copyright © 2013 by W. H. Freeman and Company Molecular Cell Biology, ...
Date added: February 13, 2013 - Views: 30
Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Konkuk university Created Date: 1/20/2002 6:56:56 PM Document presentation format
Date added: May 2, 2013 - Views: 21
Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs (Bacterial artificial chromosomes) Large low copy number plasmids (have ori and ...
Date added: October 16, 2011 - Views: 189
The gene is inserted into a vector (plasmid, virus, cosmids) that will insert the DNA into a cloning host. Cloning host is usually bacterium or yeast that can replicate the gene and translate it into a protein product.
Date added: December 13, 2011 - Views: 74
... Default set-up and AutoSeq1 Cosmids, Bacs, Genomic: BAC DSRG Profile 96° 5min 96 ° 30 sec 50 ° 20sec 60 ° 4 min C.S. Rxn conditions DNA- 1ug 10 pmol Primer straight BDv3.1 BAC’s: Modified Set-up, BAC profile BAC Sequencing: LOR ...
Date added: July 26, 2013 - Views: 18
H3 Cosmids, YACs and BACs — Cloning large DNA fragments Analysis of eukaryotic genes and the genome organization of eukaryotes requires vectors with a larger capacity for cloned DNA than plasmids or phage Human genome (3 x 109 bp ...
Date added: February 13, 2012 - Views: 10
Cosmids- artificially generated ... Radiolabeled vs. fluorescent tag Isolation of Gene of Interest Isolation of Gene of Interest Use of Antibodies Ab ppt protein Protein being synthesized on mRNA Generate cDNA from mRNA reverse transcriptase DNA polymerase Must have protein in ~pure ...
Date added: May 2, 2013 - Views: 33
... to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...
Date added: October 6, 2011 - Views: 24
... Cloning Vectors Single Cleavage Sites High Copy numbers Insertion Inactivation Lambda Phage Vector Stable Packaging Cosmids Other Vectors Expression Vectors Secretion Vectors Shuttle Vectors Bacteriophage M 13 DNA Sequencing Yeast Artificial Chromosomes Human Genome Project Cloning ...
Date added: May 25, 2013 - Views: 10
Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1.
Date added: October 7, 2011 - Views: 248
... Drosophila Genome Chromosome Morphology Chromosome Morphology Naming Chromosome Parts Drosophila X Chromosome 2b Cosmids An Inverted Repeat May Account For Strange Morphology At X2B PowerPoint Presentation PowerPoint Presentation ...
Date added: December 10, 2013 - Views: 3
... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages Molecular Cloning: Cloning Vectors 2. Bacteriophages: example is lambda 1/3 of genome ...
Date added: May 6, 2013 - Views: 2
Lecture # 04 Cloning Vectors Recombinant DNA Technology Common General Cloning Strategy Target DNA from donor organism extracted, cut with compatible restriction endonuclease and ligated into a cloning vector.
Date added: February 18, 2014 - Views: 8
... “essential genes” complete genome is mapped and sequenced entire genome is available as cosmids allows any gene to be manipulated and reintroduced into the genome C. elegans and cell behavior (contd) only about 1000 somatic cells and 1-2000 germ cells anatomy is relatively simple ...
Date added: May 8, 2012 - Views: 4
Need to clone the region between these genes walking by cosmids over 500 kbp problem with uncloneable repeated sequences: needed to jump over them. Cut genomic DNA into large fragments, circularize it and clone the junction fragments.
Date added: June 28, 2012 - Views: 24
... labeled nucleic acid with sequence matching the gene of interest Cosmids Cosmids are designed for cloning large DNA fragments Behave as plasmid and phage Contain cos sites, ...
Date added: July 14, 2014 - Views: 6
... Created a cosmid library from the YAC clone Cosmid subclones used to screen a cDNA library of human fetal brain RNA The cosmids hybridized to a portion of a gene designated as FMR1 Pedigree of a family with the Fragile X mutation segregating FMR1 Expression Northern Blot of FMR1 was done ...
Date added: August 12, 2014 - Views: 1
AD000092 /FEATURE=cds#6 /DEFINITION=CH19HHR23 Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, CRTC, and RAD23A genes, genomic sequence. 1753_s_at.
Date added: June 22, 2012 - Views: 39
Recombinant DNA Technology for the non-science major.
Date added: May 8, 2014 - Views: 4