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Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153
Date added: October 24, 2011 - Views: 52
Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...
Date added: August 2, 2013 - Views: 4
Vectors for large DNA inserts: Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b-lactamase: breaks down ampicillin ...
Date added: May 13, 2013 - Views: 26
... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti ...
Date added: August 2, 2013 - Views: 14
BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.
Date added: October 7, 2011 - Views: 428
... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...
Date added: January 13, 2013 - Views: 21
Chromosome walking can ... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs ...
Date added: October 12, 2011 - Views: 19
... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...
Date added: August 8, 2014 - Views: 3
Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids ... Molecular Cloning Author: Lab Last modified by: Pierriden Perez Created Date:
Date added: November 5, 2011 - Views: 87
Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...
Date added: December 2, 2011 - Views: 95
Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation ...
Date added: February 13, 2013 - Views: 38
Genome Sequencing Bacteriophage fX174, the first genome to be sequenced, is a viral genome with only 5,368 base pairs ... a set of cDNA sequences to a set of cosmids, ...
Date added: September 12, 2011 - Views: 59
Central Dogma of Biology DNA RNA Protein
Date added: October 13, 2014 - Views: 3
... of 2 million plus reads derived from low copy BACs defined by Dr. Tabata et al. 3) identification of low copy cosmids (US) ...
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... phage, cosmids, BACs, YACs (Table 4.2, p. 37) DNA libraries - genomic, cDNA Nucleic acid probes - cloned DNA or synthetic oligos “Instant” cloning ...
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... to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...
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... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...
Date added: May 6, 2013 - Views: 3
... but not in the middle Cosmids A cosmid is a plasmid with the l phage cos site included in it The cos site signals for packaging into l phage, ...
Date added: December 10, 2013 - Views: 3
Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3. Artificial Chromosomes YAC (yeast artificial Chromosomes - 500 ...
Date added: March 14, 2012 - Views: 78
... library of clones 10kb library of clones 150kb library of clones * C. Elegans 100Mb Drosophila 120Mb Human 3,200Mb Cosmids, ...
Date added: November 23, 2014 - Views: 1
Viral Vectors May 8 Viral vectors ... cell-cell spread suppression of class I MHC promoter pA pack HSV-ori bacterial plasmid seq cloning site defective virus cosmids ...
Date added: February 19, 2012 - Views: 39
Title: Basic Molecular Biology Information Author: t80maj1 Last modified by: t80maj1 Created Date: 8/21/2006 4:36:06 PM Document presentation format
Date added: October 29, 2011 - Views: 74
... Progress to date: 105 of 319 cosmids fully processed 11493 independent insertions 10459 insertions in 2520 orfs (of 7825 in total) 4.2 ...
Date added: May 23, 2014 - Views: 5
Title: PowerPoint Presentation Author: Wenun Last modified by: Wenun Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show
Date added: May 31, 2013 - Views: 2
Whole Genome Shotgun Sequencing cut many times at random genome forward-reverse linked reads plasmids (2 – 10 Kbp) cosmids (40 Kbp) ...
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Chapter 10: Chromosomes and chromosome errors Making chromosome preparations Normal karyotypes from domestic animals Chromosome errors Identification of chromosomes ...
Date added: May 3, 2013 - Views: 1
Lambda RED Recombination JIC in Norwich University of Tübingen
Date added: May 15, 2013 - Views: 2
Title: PowerPoint Presentation Author: Wenun Last modified by: buuic Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show (4:3)
Date added: September 15, 2012 - Views: 2
... (Cloning Vectors) 噬菌體與 cosmids 舉例 轉殖載體 (Cloning Vectors) 植物基因轉殖與 Ti 質體 轉殖載體 (Cloning Vectors) ...
Date added: March 29, 2012 - Views: 2
Vectors for different situations. Vectors for Bacterial Cells . Plasmid Vecors. Bacteriophage Vectors . Cosmids. Phagemids. Yeast Cloning Vectors. The 2μ Circle
Date added: August 2, 2013 - Views: 5
Title: PowerPoint Presentation Last modified by: Yizhang Created Date: 1/1/1601 12:00:00 AM Document presentation format: 全屏显示(4:3) Other titles
Date added: November 11, 2011 - Views: 13
... various cosmids localized to chromosome 4. b) Lanes 2-14: cosmids localized to chromosomes other than 4. The positive control in lanes 1 and 15 is cosL7L DNA . ...
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Introduction to Hierarchical Clustering ... marron/Stat321FDA/RimaIzempresentation.ppt Nearest ... sapiens DNA from chromosome 19p13.2 cosmids ...
Date added: November 14, 2011 - Views: 94
... cloning limit: 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) ...
Date added: February 18, 2014 - Views: 12
... Cloning by cosmids. The cosmid is cut at a BglII site next to the cos site. Donor genomic DNA is cut using Sau3A, which gives sticky ends compatible with BglII.
Date added: April 27, 2015 - Views: 1
FISH 세개의 sequenced DAZ cosmids를 FISH를 위한 probes로 이용하였음. 두개의 연결된 DAZ 유전자의 5번 말단 부위에 위치한 Cosmid 18E8 ...
Date added: September 18, 2012 - Views: 7
* Cloning by cosmids. The cosmid is cut at a BglII site next to the cos site. Donor genomic DNA is cut by using Sau3A, which gives sticky ends compatible with BglII.
Date added: September 5, 2013 - Views: 1
... 载体 to integrate a gene in a genome through a vector Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors ...
Date added: March 2, 2012 - Views: 9
... Cosmids * cohesive ends of l phage DNA * plasmid origin of replication * up to 40-50 kb of DNA can be cloned M13 phage vectors * derived from filamentous ...
Date added: October 29, 2012 - Views: 21
Common vectors used in libraries Plasmids Up to 10kbp inserts Modified Lambda phage Up to 20kbp inserts/40kbp for cosmids Artificial cloning vectors BAC- Vectors ...
Date added: February 8, 2014 - Views: 13
Lambda Zap Cosmids La sonde ‘a’ identifie les clones 1 et 2 Une sonde faite à partir de la séquence le plus 3’ du clone 2 va identifier les clones 3 et 4 Les ...
Date added: February 3, 2014 - Views: 1
Michael Kovshilovsky Swapna Annavarapu SoCalBSI 2005 Internship site: BioDiscovery, Inc. Mentor: Dr. Bruce Hoff Source of Funding: BioDiscovery, Inc. Motivation ...
Date added: August 24, 2014 - Views: 7
Capturing User Intent for Information Retrieval Hien Nguyen University of Connecticut Major advisor: Dr. Eugene Santos Jr. (UCONN) Associate advisor: Dr. Robert ...
Date added: July 3, 2014 - Views: 1
cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be
Date added: October 7, 2011 - Views: 227
E. coli cloning vector: plasmids, bacteriophages (l and M13), plasmid-bacteriophage l hybrids (cosmids). Yeast cloning vector: yeast artificial chromosomes ...
Date added: November 27, 2013 - Views: 11
Chapter 4 Molecular Cloning Methods
Date added: May 10, 2013 - Views: 20
Estudi del genoma de Streptomyces coelicolor A3(2) Marcel·la Vidal Gabarró Mòdul Genòmica i Proteòmica Màster en Biotecnologia Avançada, UAB
Date added: May 27, 2015 - Views: 1
... (gamma-PPT-A) gene, partial cds. ... extracted from Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, ...
Date added: August 24, 2014 - Views: 13
... 检测存在基因组内的单一序列基因，最有效的方法是应用含大插入片段的克隆载体，如全Cosmids（载约40kb的插入片段），更 ...
Date added: May 3, 2013 - Views: 3