Scott A. Barros, PhD, DABT Sr. Scientist, Toxicology What is an Aptamer? Aptamer Structure Medicinal Chemistry Process Increased plasma stability Increased affinity Increased potency Considerations in Safety Assessment of Aptamers In general, aptamers have toxicological properties similar to ...
Date added: October 30, 2011 - Views: 108
... • Structural studies of aptamer-ligand complexes have provided a wealth of information regarding RNA structure and ligand interaction • Most aptamers exhibit conformational changes upon binding to ligand (induced fit binding) ...
Date added: October 31, 2011 - Views: 61
Impact Aptamer recognition elements enable detection of nucleic acids, proteins, small molecules, inorganic ions Accomplishments Smart electrode surfaces allow real-time detection of small molecule analytes Device integration: ...
Date added: October 12, 2011 - Views: 55
Factor IXa Inhibition Aptamer Technology Overview and First Results with RB006/RB007 Mauricio G. Cohen, MD Associate Professor of Medicine Director, Cardiac Cath Lab
Date added: November 8, 2011 - Views: 37
The Aptamer Database is a comprehensive, annotated repository for information about aptamers and in vitro selection. This resource is provided to collect, organize and distribute all the known information regarding aptamer selection.
Date added: June 11, 2013 - Views: 5
... ErbB3 Targeted Aptamer-Nanoparticle Conjugate, Massachusetts General Hospital, Boston, Mass. Carolina Center of Cancer Nanotechnology Excellence, University of North Carolina, Chapel Hill, N.C. Nanosystems Biology Cancer Center, ...
Date added: June 26, 2012 - Views: 38
Design options A: Aptamer-based Good: It’s simple. It’s been done before with success. Bad: Requires design of different aptamer sequences that can bind to cell-surface protein. B: Biotin/Streptavidin Good: Depends on association b/w biotin and streptavidin.
Date added: March 31, 2012 - Views: 10
He, et al., Chemical Science, 2011 Aptamer-Based SERS Assay L. He, et al., Chemical Science, 2011 Raman shift (cm-1) Assignments Aptamer (*) 1316 Adenine (ring stretching) 1022 DNA backbone(PO2- stretching) 792 thymine, cytosine 735 Adenine (ring breathing) ...
Date added: November 25, 2011 - Views: 13
Reserve your “block” now We have developed a proprietary, high-throughput process for DNA aptamer production. Supply any protein target, and receive a custom, high affinity DNA aptamer in just weeks.
Date added: February 17, 2014 - Views: 1
Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors Kenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura,
Date added: November 30, 2012 - Views: 15
WHAT FACTORS PROMOTE THE EMERGENCE OF BIOCOMPLEXITY? Robert M. Hazen, Carnegie Institution Kavli Futures Symposium – Bio & Nano June 13, 2007
Date added: December 29, 2013 - Views: 2
… an ‘expression platform’ for gene regulation. A metabolite-binding ‘aptamer’ domain and … Riboswitch Functions U U U U U X 3 4 2 1 Direct (protein-free) binding of metabolite to RNA Metabolite-dependent regulation of genes These two activities are accomplished by two functionally ...
Date added: February 2, 2012 - Views: 20
Soontornworajit, B., Zhou, J., Zhang, Z., Wang, Y. Aptamer-functionalized in situ injectable hydrogel for controlled protein release. Biomacromolecules. 2010; 11: 2724-2730.
Date added: September 9, 2014 - Views: 1
... 657 RNA aptamers -- antibody-like properties Vitamin b12-binding RNA aptamer Slide 10 Let the immune system make enzymes for you Wedemayer et al. (1997) Science 276, 1665-1669. Catalytic antibody that hydrolyzes cocaine Zhu et al., 2006, ...
Date added: January 29, 2013 - Views: 19
One anti-Rev aptamer: binds peptide in alpha-helical conformation Another anti-Rev aptamer: binds peptide in an extended conformation MS2 protein as beta sheet bound via protruding side chains DNA synthesizer T7 prom PCR site random 1015 T7 polymerase, ...
Date added: May 5, 2013 - Views: 38
Aptamer antagonist of tissue factor pathway inhibitor. Improved thrombin generation in plasma from subjects with FVIII deficiency. Improved clot times as measured by thromboelastography in monkeys depleted of FVIII. Waters, Blood, 2009. Fucoidans.
Date added: April 19, 2013 - Views: 18
... at the transcriptional level Others can function at the translational level Model of Riboswitch Action FMN binds to aptamer in a riboswitch called the RFN element in 5’-UTR of the ribD mRNA Binding FMN, ...
Date added: August 9, 2014 - Views: 1
S-aptamer-tagged ARE and mtARE reporters were used for in vivo Crosslinking-coupled RNP purificaiton followed by RPA assay High-mobility group A2 (HMGA2) 3’ UTR contains seven sites for the endogenous Let-7 High-mobility group A2 (HMGA2) ...
Date added: June 1, 2013 - Views: 5
... Another RNA aptamer example: adenosine aptamer Class II ligase ribozyme Pitt & Ferré-D’Amaré, J. Am. Chem. Soc., 2009, 131 (10), pp 3532–3540 Class II ligase ribozyme Rapid Construction of Empirical RNA Fitness Landscapes Jason N. Pitt and Adrian R. Ferré-D’Amaré* Science 2010: ...
Date added: December 10, 2013 - Views: 2
REG1: Pegnivacogin + AnivamersenRegado Biosciences, Inc. Novel Anticoagulation System. RNA aptamer . Factor IXa inhibitor. Controlling agent. Active. Specific
Date added: November 10, 2011 - Views: 11
... First Anti Angiogenic Agent 28 Base RNA Aptamer NON-IMMUNOGENIC NATURE Selectively binds extra cellular VEGF 165 DOES NOT EFFECT NORMAL VASCUALR GROWTH BEVACIZUMAB (AVASTIN) Humanized mono-clonal antibody. Active Against all ...
Date added: June 2, 2013 - Views: 31
... Multiple Isoforms of VEGF-A Are Generated from Exon Splicing VEGF Inhibition in AMD FDA approved Pegaptanib Aptamer Specific for VEGF-A isoform 1651 Ranibizumab Recombinant, ...
Date added: October 16, 2011 - Views: 81
Class 27_11 last updated 12/14/11 12:30 PM Limitations of siRNA silencing in mammalian cells Transient nature of the response (~3 days) Transfection problems (cell type, refractoriness)
Date added: January 27, 2012 - Views: 17
VWF Aptamer and Murine Carotid Injury Nimjee S et al. Mol Therapy 2011 Universal Antidotes for VWF Aptamer CDP and VWF Aptamer PDA-DPA and VWF Aptamer Oney S et al. Nature Medicine Oct 4, 2009. Extracellular DNA Traps and Thrombosis Fuchs. PNAS 2010;107 ...
Date added: May 5, 2013 - Views: 13
... Intracellular Lung Tarceva, Iressa Aptamer Cell Surface, Intracellular Renal, Lung AS1411 (in dev.) Peptide Cell Surface, Intracellular Breast, Thyroid RGD (in dev.) Targeting Agent Mode of Action Cancer Application Known Example(s) ...
Date added: November 11, 2011 - Views: 30
... Intracellular Peptide AS1411 (in dev.) Cell Surface, Intracellular Aptamer Her2, Erbitux Cell Surface Monoclonal Antibody Functionality Dispersion - Effective aqueous dispersion must be demonstrated. Preliminary results are encouraging. (internal research) ...
Date added: November 5, 2011 - Views: 25
... Selection against proteins - Selection of new ribozymes (RNA world) The ATP aptamer structure Nucleolin RNA Targets B1 10-50nM (5’ETS) Mouse B2 50-100nM (5’ETS) ...
Date added: October 7, 2011 - Views: 44
aptamer. fragments. Anal Method . ... Lead: 100 ppt (2 orders of magnitude below the EPA standard limit) 2-3 orders of magnitude. Small molecules. 7 nM (5 orders of magnitude more sensitive than the colorimetric method) > 4 orders of magnitude. Explosive chemicals.
Date added: June 10, 2013 - Views: 15
CU Boulder Research, Scholarly Activity, and Creative Works GSAC April 30, 2010 Russ Moore, Interim VC Research Research & Scholarly Works Remarkable record of research and scholarly works achievement across all schools and colleges on our campus see http://www.colorado.edu/research/achievements ...
Date added: August 1, 2013 - Views: 269
Terminator Aptamer Research focus on riboswitches Bioinformatics Without TPP What are Riboswitches? Anti-terminator Aptamer Vitamin B1 biosynthesis in bacteria: TPP-mediated attentuation control: E. Nudler and coworkers: (Mironov et al., ...
Date added: July 30, 2013 - Views: 5
... 49-60 Aptamer remains receptive to FMN binding until it is disrupted by the formation of the antiterminator helix. = irreversible event of the riboswitch at low FMN concentrations longer transcripts have lower affinity aptamers of transcripts paused at PA and PB have affinities better ...
Date added: December 14, 2011 - Views: 25
Research focus on riboswitches Bioinformatics Searching for riboswitches in eukaryotes by their aptamer Sequence is not enough, need structure considerations! Research focus on riboswitches Bioinformatics Searching for riboswitches in eukaryotes by their aptamer (Cohen et al.,
Date added: August 31, 2013 - Views: 1
LEADERSHIP STEPS Understand the relevant research (UTeach/FRI), seek informed guidance (Master teachers—UTeach) Consult/involve faculty leaders (UTeach) or support a faculty initiative (FRI)
Date added: September 21, 2013 - Views: 2
... Factor IXa inhibitor (REG1) RB006, Injectable RNA aptamer that specifically binds and inhibits factor Ixa RB007, the complementary oligonucleotide that neutralizes its anti-IXa activity if & when needed Factor XI inhibitor Antisense oligonucleotide targeting factor XI ...
Date added: May 2, 2013 - Views: 20
Indicator Displacement Assays for Solute Sensing Julee Byram Mecozzi Group May 10, 2007 * * * * * * * * * * * * * * * * * * * * * * * * * Aptamer-Based Sensor for Cocaine Stojanovic, M.N.; Prada, P.; Landry, D.W. J. Am. Chem. Soc. 2001, 123, 4928 518 nm 472 nm Kd ~ 100 μM Cocaine concentration ...
Date added: November 2, 2011 - Views: 17
... Binds specific ligand Theophylline Theophylline aptamer Caffeine Synthesize de novo Terminator + YFP Add antiswitch On Off ‘Off’ antiswitch ‘On’ antiswitch Verify construction PCR products PCR products + Promoter ON OFF Caffeine Detector “Digital Decoder Device” Goal: ...
Date added: September 17, 2013 - Views: 6
An aptamer for a desired target molecule is chosen from a pool of random DNA or RNA sequences by successive cyclings of ... Arial Times New Roman Verdana Wingdings Globe Applications of Spectrophotometry Analysis of a Mixture Slide 3 Slide 4 Slide 5 Slide 6 Measuring an ...
Date added: April 23, 2012 - Views: 31
The portion that directly binds the ligand is the aptamer. Expression can be affected transcriptionally or post-transcriptionally. The ribD operon controls synthesis and transport of riboflavin and FMN.
Date added: April 12, 2012 - Views: 18
Study of the interaction between the porphyrin TmPyP4 and the Thrombin Binding Aptamer (TBA) G-quadruplex Miquel del Toro1, Ramon Eritja2, Raimundo Gargallo1, ... guanine3 guanine4 pKa = 10,5 0,1 TmPyP4 Melting Acid base titration EXPERIMENTAL
Date added: March 7, 2012 - Views: 16
The aptamer domain is a highly folded structure that selectively binds to the target metabolite. The expression platform converts metabolite binding events into changes in gene expression by harnessing changes in RNA folding that are brought about by ligand binding.
Date added: May 25, 2013 - Views: 30
PEG PLGA Aptamer … + + + + Precipitate nanoparticles by dilution in water Mix in selected ratio Synthesis of 100 types of nanoparticles per minute Drug COOH NH PEG PLGA Aptamer … COOH NH PEG PLGA Aptamer … PEG PLGA Aptamer …
Date added: November 25, 2011 - Views: 41
... Aptamer domain (responsible for binding of ligand) Expression plataform (responsible for impacting gene expression) Structure Mechanism Three known mechanisms: Formation of intrinsic terminator stem (inhibits transcription by inducing its termination) ...
Date added: May 11, 2013 - Views: 20
... 8% -> 96% REGULATION OF GENE EXPRESSION ANTISENSE STRATEGY interaction with RNA ANTIGENE STRATEGY interaction with DNA APTAMER STRATEGY interaction with proteins * ...
Date added: December 21, 2013 - Views: 3
Presently involved in aptamer, peptide, antibody and plasma-deposited hydrogels research. Currently developing an RFID/Biosensor. Technology Background (3) RFID/Biosensor An interrogation unit transmits an RF signal to an RFID/Biosensor.
Date added: May 2, 2013 - Views: 4
- 2013/2014 - 3D Structures of Biological Macromolecules Part 3: Nucleic Acids Jürgen Sühnel [email protected] Leibniz Institute for Age Research, Fritz Lipmann Institute,
Date added: April 19, 2014 - Views: 6
Discuss different stems and discuss the single and anti-sense stem first, then mention aptamer stem -----> aptamers Antiswitch RNA molecules are too small to and require an exact sequence, they cannot be made directly in the cells.
Date added: September 13, 2013 - Views: 3
MG, the ligand for which the aptamer was originally selected, consistently has the more favorable enthalpy (heat) as well as a larger heat capacity (the dependence of enthalpy on temperature).
Date added: September 21, 2014 - Views: 1